We narrowed to 888 results for: URE

...started with tissue culture The cultivation of mammalian cells in the lab, or tissue culture as it is commonly...antibodies or viruses. If you’re new to tissue culture, check out this video for some tips and best practices...practices for getting started with tissue cultures. To keep up on all of the latest protocol videos...

...Massachusetts that means it’s time for near-freezing temperatures, our first of the season snow flurries, dusting...holiday season! It is that time of the year again: miniature #Christmas tree goes up at home!This year with...December 4, 2020 Here’s how to enter: Post your picture or video for either contest on Twitter or Instagram...follow us on Twitter and Instagram as we share pictures of our decked out spaces.       ...

...recommend you read the article to ensure you understand the features of the plasmids, how they were created... to the PubMed Preferences and Filters pages, be sure to check out the other “LinkOut” badges you can ...Addgene Blog Get Tips on Keeping Up with the Literature How to Write a Scientific Review Article Tips...

... plasmids shipped daily may be easy to picture (see the picture of our shipping room above), but as Joanne...amongst the lab team, shipping team, and others to ensure that everything will ship out in time - from assembling...said during our celebratory lunch, it’s hard to picture one million plasmids. How much space would one ...

... of a scientist was like. The word “scientist” conjured up images of men like Albert Einstein and Bill... make decisions about what we want to do in the future. How do I get started with research? I had no idea...personal interests While it’s perfectly fine to be unsure, it helps to have a vague sense of what kind of..., even if a question is a very basic one, making sure that you have a good fundamental understanding of...goals helped me reflect on what I wanted for my own future. Informational interviews with people who have ...

...lucky enough to have an imaging core or tissue culture core, you are looking at a process-oriented team...come across one. Also, remember, one important feature that all projects have in common - and by association...is a blog post, so here are just a few critical features based on my own experience: Leadership Few things...certainly necessary, but equally important is making sure all team members understand what roles they’re meant...well, you can learn from the experience and make sure your next team works better. In fact, don’t wait...

...breakthroughs and undermine critical research infrastructure that has taken decades to establish.These funding...materials that might otherwise be lost amid lab closures or project terminations, ensuring that scientific...grant applications, addressing the new funding structures and indirect cost caps more efficiently. Offering...can navigate this difficult period together. The future of scientific progress depends on our collective...

...Exciting news! Addgene recently rolled out a new feature on our plasmid pages - links to articles citing...screenshot below to see what I mean. Additional features Once you've clicked on the "# References" link...article abstract and more. We've also included a feature to help save you from performing any unnecessary...domains" publication.  If you want to see this new feature in action, click here to explore the TetO-FUW-OKSM...

...Doudna and Eva Nogales presented the first detailed picture of Cas9's three-dimensional shape via x-ray crystallography...Cas9's structural details. "Understanding this structure may help us engineer around the current limitations...in Feng Zhang's lab, in a press release. "In the future, it could allow us to design versions of these ...University summed it up well in that New York Times feature when he said, “All of this has basically happened...

...Live-cell measurements of kinase activity in single cells using translocation reporters. Nature protocols...whose cellular localization serves as a proxy measurement of kinase activity. The key advantage of KTRs...nucleus, while FRET-based reporters can be used to measure kinase activity in specific subcellular compartments...

... useful tools because we can easily detect and measure the light they emit. Proteins that give off light...observed in the crystal jellyfish Aequorea victoria (Figure 1) in molecular detail. On the underside of the...this new green glowing protein could be used to measure gene transcription, as well as to track protein...GFP’s function. His lab described the protein’s structure and also discovered many mutations that alter ...

... a dish that features that ingredient. We just had a cook-off last week, and the featured ingredient was...Harry Potter-style.The shared lunches create a culture of openness and trust that comes from caring about...ll dedicate a post to these competitions in the future, and hopefully include some of our favorite winning...

...heart health. These are amazing advances that will surely save thousands if not millions of lives, but many...resources and promoting research for rare disease cures. Through their BeHEARD Award, the Rare Genomics ...better choose which genes and pathways to target in future small molecule screens. He plans to work with a...voice. Check back with Addgene often to learn of future rare disease research funding opportunities. References...

...values with each element. We also set out to make sure the page looked clean, professional, and modern....our website, or in social media, we wanted to make sure our friend matched our core branding. Blugene needs... our values of community and sharing.  One big feature upgrade? Blugene now has a full expression. You...Addgene's new search tool Read more about other features of Addgene's website Resources on Addgene.org...

...Ashley Waldron Organoids are three-dimensional cultures that mimic aspects of organ morphology and physiology... an organoid model (Sun, et al. 2021).  Figure 1: Example schematic from the Organoid EasyTag ...targeted cells that can be used to generate organoid cultures with a modification of interest. While the authors...other Cas12a nucleases identified, functions at temperatures up to 43 °C and can be used for genome editing...

...Rodríguez et al., 2011).  Genetics of SPARC Figure 1: SPARC and SPARC2 use the GAL4-UAS system along...sparse (S) labeling of the cell population. Figure 2: Dense (c), intermediate (d), and sparse (e) ...gene expression and clonal analysis in Drosophila. Nature Methods 9:47–55 . https://doi.org/10.1038/nmeth...projection-specific brain-wide reconstruction of single neurons. Nature Methods 15:1033–1036 . https://doi.org/10.1038/...

...That includes keeping fees as low as possible to ensure that our materials are affordable but enough to...community-oriented values. Addgenies like the nonprofit company culture as well. We work every day to do a better job of... our core values. These accurately reflect our culture and outlook: Addgene increases productivity by...increase sharing in the community Addgene fosters a culture of respect, collaboration, and fun Addgene believes...

... of the brain where DA quantitation is desired (Figure 1). The probe perfuses liquid, which causes the... the method is powerful and offers immediate measurements in a live animal that can be correlated to behavior...developed fluorescent-protein based DA sensors (Figure 2). The dLight1 dopamine sensor In 2018, Lin Tian...powerful new developments in the detection and measurement of neurotransmitters in various species and while...

...wanted to be. But prior to graduate school, I wasn’t sure a master’s was a good decision. So how do you know...means to gain employment, so it’s important to make sure a masters helps you get a job you want. But how ...with master's have Job descriptions often fail to capture all of the responsibilities of a role, so it helps...questions in this blog post can help you start to figure out if a master’s is a good fit with your interests...

...boxes). We get these boxes a lot when receiving temperature-controlled reagents. Expanded polystyrene will...company and it has to be packaging (no coated tissue culture plates, for example). Check the link for more details...or Axygen®, set up a bin or box in your tissue culture room for the wrappers and ship them back. Start...make a huge impact directly on waste, but also on culture. Encourage your workplace to become a leader in...