We narrowed to 664 results for: cell

1. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

   Type

   Blog Post

   Published

   June 8, 2023, 1:15 p.m.

   ...approach for deep phenotyping and spatial analysis of cells in complex tissues. Proceedings of the National ...

2. Viral Vectors 101: Parts of the AAV Packaging Plasmid

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   Blog Post

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   Dec. 3, 2020, 2:15 p.m.

   ...for serotype-specific differences in tissue and/or cell type tropism. Introducing changes and/or short peptides...

3. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

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   Blog Post

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   June 23, 2020, 1:15 p.m.

   ...SARS-CoV-2 proteins in bacteria, yeast, and mammalian cells. We teamed up with Ginkgo Bioworks to quickly bring...

4. London Calling - Addgene’s New Europe Office in London

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   Blog Post

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   June 10, 2014, 3:20 p.m.

   ...anti-tumour therapy using antibodies targeting dendritic cells. These genetic tools were pivotal in carrying out...

5. The Stingy Scientist: How the Baby Gel Box Was Born

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   Blog Post

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   April 15, 2014, 3:11 p.m.

   ...recently shared our protocol for making competent cells to save time and money. My other stingy science ...

6. Tag Your Favorite Yeast Genes with Ease

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   Blog Post

   Published

   Nov. 19, 2013, 2:37 p.m.

   ...insertion. The linear PCR product is introduced into the cell by direct transformation. A given insert will typically...

7. Year of the Camelid: Antibody Style

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   Blog Post

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   May 9, 2024, 1:15 p.m.

   ...antigens in living cells or tissues. sdAbs can also be used to manipulate cellular functions, like in ...sdAbs over conventional antibodies include:  Excellent tissue penetration, thanks to their small size...

8. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

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   Blog Post

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   March 15, 2018, 12:59 p.m.

   ...experiments to test nickase preferences using HEK293 cells. They started by comparing D10A and H840A total ...

9. New Norepinephrine Indicators: nLightG and nLightR

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   Blog Post

   Published

   Dec. 12, 2023, 2:15 p.m.

   ...al., 2022). B, Representative images of HEK293T cells and neurons expressing nLightG or nLightR before...

10. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

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    Blog Post

    Published

    Sept. 12, 2023, 1:15 p.m.

    ... repository of CRISPR screens Cellosaurus: A knowledge resource for cell lines IBEX Knowledge Base: A ...

11. Sensing Neuronal Dopamine

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    Blog Post

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    July 30, 2019, 12:59 p.m.

    ...detection of dopamine in flies, fish, and mice." Cell 174.2 (2018): 481-496.PubMed PMID: 30007419. PubMed...the brain has been in vivo microdialysis of extracellular fluid (Darvesh et al., 2011). This technique... across the semipermeable membrane from the extracellular fluid into the perfused liquid. The probe then...

12. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

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    Blog Post

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    April 25, 2019, 3:01 p.m.

    ...a FRET donor to mCitrine and mOrange or for live cell imaging of fusions and FRET reporters. mTFP1 is ...

13. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type

    Blog Post

    Published

    March 5, 2020, 2:15 p.m.

    ... expression after AAV injection, what region and cell class ended up expressing your virus? If you were...

14. New and Upcoming Viral Vectors - December 2019

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    Blog Post

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    Dec. 19, 2019, 2:40 p.m.

    ... results, but also confirm expression in target cells. While the various AAV serotypes can be used to ...

15. With an Eye Towards the Future, We Look Back at the March for Science

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    Blog Post

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    Oct. 24, 2017, 1:54 p.m.

    ...fellow at Vanderbilt University. She studies beta-cell function and failure in Type 2 Diabetes. Birmingham...BC (Canada). She works in a lab that uses yeast cells to study how DNA damage promotes cancer. She is ...

16. Science Careers: Unruly Interests Feed Many Paths

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    Blog Post

    Published

    Sept. 26, 2017, 12:12 p.m.

    ...sciences, viewed through the lens of development, cell, and molecular biology. Beyond that, she is also...

17. FPbase: A new community-editable fluorescent protein database

    Type

    Blog Post

    Published

    May 16, 2018, 1 p.m.

    ...Associate at Harvard Medical School. He manages the Cell Biology Microscopy Facility, specializing in advanced...  There are many online guides – including an excellent series of posts by Joachim Goedhart on the Addgene...

18. In Vivo Biotinylation of Bacterial Fusion Proteins

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    Blog Post

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    Jan. 25, 2018, 2:09 p.m.

    ... Eye Institute and is particularly interested in cell signaling.     Additional Resources on the Addgene...successfully used this plasmid to biotinylate the extracellular domain of the erythropoietin receptor and subsequently...

19. Multifunctional Neural Fibers: Modulation from Gut to Brain

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    Blog Post

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    Aug. 31, 2023, 1 p.m.

    ...and chemically excited a class of enteroendocrine cells within the intestines known as neuropods. They observed...

20. New and Upcoming Viral Vectors - September 2019

    Type

    Blog Post

    Published

    Sept. 12, 2019, 1 p.m.

    ...recommend validating the serotype in your target cells before conducting the experiment. Likewise, a lot...