We narrowed to 766 results for: form

1. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

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   Blog Post

   Published

   Oct. 3, 2017, 12:11 p.m.

   ...goes on our website. We look at product pages, performance data, protocols, and even certificates of analysis...Addgene Blog Get tips on how to do your own informational interview Check out this post if you debating...

2. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

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   Blog Post

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   July 2, 2015, 8:37 p.m.

   ...fulfill its mission of helping scientists share information and, of course, plasmids. My primary duties are...presenting, analytical thinking, time management, information distillation, etc) are transferable to many different...

3. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

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   Blog Post

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   June 27, 2018, 1:16 p.m.

   ...isolated from Eubacterium rectale, significantly outperforms other RTs in this regard and is even able to ...antibodies. These properties make nanobodies easier to transform and express in bacteria, which, in turn, makes...

4. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

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   Blog Post

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   Aug. 8, 2019, 1:19 p.m.

   ...help spot inconsistencies in the work, missing information that would prevent the study to be replicated...comments to “you” I can easily check if I would have formulated specific comment differently if I would be speaking...

5. Fluorescent Biosensors for Measuring Autophagic Flux

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   Blog Post

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   Jan. 22, 2019, 2:41 p.m.

   ...a static measurement and it provides limited information about what materials or organelles are being ... acidic pH found in lysosomes. Therefore, the formation of autophagosomes leads to an increase of yellow...

6. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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   Blog Post

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   Dec. 13, 2018, 1:14 p.m.

   ...viral service but did you know that Addgene also performs independent research? Since launching the viral...sorbitol is already a common excipient in AAV formulation buffers, this sugar may be a more attractive ...

7. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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   Blog Post

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   June 9, 2016, 2:30 p.m.

   ...choosing a genomic target. NgAgo also appears to outperform Cas9 in GC-rich regions. DNA guides NgAgo uses...re looking to disrupt gene function via indel formation, this property could be an advantage. However,...

8. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

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   Blog Post

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   Oct. 29, 2015, 2:30 p.m.

   ...pSC102, with EcoRI and selected for E. coli transformants that were resistant to both. When this proved... Central PMCID: PMC427208. Novick, R P et al. “Uniform Nomenclature for Bacterial Plasmids: A Proposal...

9. "Build Your Plasmid": The Game - Play to Teach

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   Blog Post

   Published

   July 17, 2018, 12:23 p.m.

   ...but it can be difficult to determine how much information they’ve absorbed if they don’t get a chance apply...gaps in their knowledge. Practically speaking the format I decided on was to have two teams of 4 people ...

10. FlipGFP, a novel fluorescence protease reporter to study apoptosis

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    Blog Post

    Published

    May 21, 2019, 12:10 p.m.

    ...together and force the strands into a parallel conformation. Between β11 and one of the linkers is a consensus...-11, where β10-11 was forced into a parallel conformation by the E5 and K5 linkers that are connected ...

11. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

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    Blog Post

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    June 20, 2017, 2:30 p.m.

    ...and the pH shift can be prevented. Keep this information in mind if you’re planning on shipping something...study found that dry ice storage affected the performance of antibodies, because of effects of pH on protein...

12. Addgene Depositors Get More Citations

    Type

    Blog Post

    Published

    April 20, 2017, 2:30 p.m.

    ... Web of Science provides basic bibliographic information and uses its vast network of articles to track...including biotech and research tools, computing performance, and randomized control trials in innovation....

13. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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    Blog Post

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    May 3, 2018, 1:48 p.m.

    ...identify Cas13d, both groups used very general bioinformatic screens that looked for a CRISPR repeat array...of Type VI Cas13d enzymes is another win for bioinformatic data mining. As we continue to harness the natural...

14. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    Blog Post

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    June 6, 2019, 12:41 p.m.

    ...application of blue light, iLID undergoes a conformational change allowing high affinity binding to SspB...These optical reporters have limited deep-tissue performance, and sometimes require an alternative such as...

15. Viral Vectors 101: Viral Vector Elements

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    Blog Post

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    July 25, 2023, 1 p.m.

    ...quite different from gamma-retrovirus, but they perform very similar functions to infiltrate the host cell...and mediate efficient viral replication. More information on these viral genes is available in the adenovirus...

16. Antibodies 101: Monoclonal Antibodies

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    Blog Post

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    June 29, 2021, 1:15 p.m.

    ...survive this step. Then, a limiting dilution is performed to isolate individual hybridomas and ELISA is ...Distinguishing Characteristics, Applications, and Information Resources. ILAR Journal 46:258–268. https://doi.org...

17. AAV Q&A with Tim Miles

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    Blog Post

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    May 17, 2022, 1:15 p.m.

    ...different doses. See Figure 4 in this paper for more information.   Q6 & Q7: We have been attempting to transduce...high coverage. Please see this paper for more information about PHP.eB brain labeling at various doses....

18. Developing a cold tolerant E. coli using specialized chaperones

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    Blog Post

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    Jan. 28, 2020, 2:35 p.m.

    ... host cells (the simplest example would be a transformed mesophilic, for example E. coli, carrying a plasmid...2019 All-India iGEM Meet-up, which served as a platform for all the Indian iGEM teams to interact with...

19. From Lab Bench to Leadership: Transitioning into Management as a PhD

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    Blog Post

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    Jan. 21, 2025, 2:15 p.m.

    ...underutilized team potential. I often thought that performing a cell culture experiment myself would take less... Kim Scott Courses: Leadership programs on platforms like Coursera, LinkedIn Learning, or edX. Podcasts...

20. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type

    Blog Post

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    Aug. 6, 2024, 1:15 p.m.

    ...products for packaging (Gag, Pol, and Env), the isoforms of these proteins, as well as the viral LTRs, ...different viruses and thus use slightly different isoforms of these packaging components. Therefore, lentiviruses...