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... they could solve this problem. D10A nickase outperformed both WT Cas9 sites tested by a wide margin, ...use Cas9 nickases in genome editing. For more information and a link to the archived webinar, click here...

...that much tissue takes a week of multiple people performing dissections. With CUT&RUN, we have had success...up until this point. The nuclease reaction is performed on ice, and only for a short period of time, thus...

...Some use cases include: Cataloging a reagent’s performance as in this antibody ReFigure Analyzing replications...confirmatory image to show that a previously used reagent performs well, or you might publish a replication experiment...

...goes on our website. We look at product pages, performance data, protocols, and even certificates of analysis...Addgene Blog Get tips on how to do your own informational interview Check out this post if you debating...

...fulfill its mission of helping scientists share information and, of course, plasmids. My primary duties are...presenting, analytical thinking, time management, information distillation, etc) are transferable to many different...

...help spot inconsistencies in the work, missing information that would prevent the study to be replicated...comments to “you” I can easily check if I would have formulated specific comment differently if I would be speaking...

...a static measurement and it provides limited information about what materials or organelles are being ... acidic pH found in lysosomes. Therefore, the formation of autophagosomes leads to an increase of yellow...

...viral service but did you know that Addgene also performs independent research? Since launching the viral...sorbitol is already a common excipient in AAV formulation buffers, this sugar may be a more attractive ...

...isolated from Eubacterium rectale, significantly outperforms other RTs in this regard and is even able to ...antibodies. These properties make nanobodies easier to transform and express in bacteria, which, in turn, makes...

...choosing a genomic target. NgAgo also appears to outperform Cas9 in GC-rich regions. DNA guides NgAgo uses...re looking to disrupt gene function via indel formation, this property could be an advantage. However,...

...pSC102, with EcoRI and selected for E. coli transformants that were resistant to both. When this proved... Central PMCID: PMC427208. Novick, R P et al. “Uniform Nomenclature for Bacterial Plasmids: A Proposal...

...together and force the strands into a parallel conformation. Between β11 and one of the linkers is a consensus...-11, where β10-11 was forced into a parallel conformation by the E5 and K5 linkers that are connected ...

...but it can be difficult to determine how much information they’ve absorbed if they don’t get a chance apply...gaps in their knowledge. Practically speaking the format I decided on was to have two teams of 4 people ...

...and the pH shift can be prevented. Keep this information in mind if you’re planning on shipping something...study found that dry ice storage affected the performance of antibodies, because of effects of pH on protein...

... Web of Science provides basic bibliographic information and uses its vast network of articles to track...including biotech and research tools, computing performance, and randomized control trials in innovation....

...underutilized team potential. I often thought that performing a cell culture experiment myself would take less... Kim Scott Courses: Leadership programs on platforms like Coursera, LinkedIn Learning, or edX. Podcasts...

...products for packaging (Gag, Pol, and Env), the isoforms of these proteins, as well as the viral LTRs, ...different viruses and thus use slightly different isoforms of these packaging components. Therefore, lentiviruses...

...quite different from gamma-retrovirus, but they perform very similar functions to infiltrate the host cell...and mediate efficient viral replication. More information on these viral genes is available in the adenovirus...

...different doses. See Figure 4 in this paper for more information.   Q6 & Q7: We have been attempting to transduce...high coverage. Please see this paper for more information about PHP.eB brain labeling at various doses....

...survive this step. Then, a limiting dilution is performed to isolate individual hybridomas and ELISA is ...Distinguishing Characteristics, Applications, and Information Resources. ILAR Journal 46:258–268. https://doi.org...