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Showing: 541 - 560 of 756 results

  1. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...pSC102, with EcoRI and selected for E. coli transformants that were resistant to both. When this proved... Central PMCID: PMC427208. Novick, R P et al. “Uniform Nomenclature for Bacterial Plasmids: A Proposal...

  2. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...but it can be difficult to determine how much information they’ve absorbed if they don’t get a chance apply...gaps in their knowledge. Practically speaking the format I decided on was to have two teams of 4 people ...

  3. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...and the pH shift can be prevented. Keep this information in mind if you’re planning on shipping something...study found that dry ice storage affected the performance of antibodies, because of effects of pH on protein...

  4. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ... Web of Science provides basic bibliographic information and uses its vast network of articles to track...including biotech and research tools, computing performance, and randomized control trials in innovation....

  5. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...together and force the strands into a parallel conformation. Between β11 and one of the linkers is a consensus...-11, where β10-11 was forced into a parallel conformation by the E5 and K5 linkers that are connected ...

  6. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    ...Renilla luciferase from the sea pansy (Renilla reniformis) emits a blue light with a peak emission at 480nm...Vargulin) luciferin None Yes Sea pansy(Renilla reniformis) 480 36 Coelenterazine None No Deep sea coceopod...

  11. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...While various Cas9 mutants have been engineered to perform unique editing tasks, classic Cas9 nuclease, which...generating a monoclonal Cas9-expression line prior to performing a screen or even a single gene knockout. Top ...

  13. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...different doses. See Figure 4 in this paper for more information.   Q6 & Q7: We have been attempting to transduce...high coverage. Please see this paper for more information about PHP.eB brain labeling at various doses....

  14. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...survive this step. Then, a limiting dilution is performed to isolate individual hybridomas and ELISA is ...Distinguishing Characteristics, Applications, and Information Resources. ILAR Journal 46:258–268. https://doi.org...

  15. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...quite different from gamma-retrovirus, but they perform very similar functions to infiltrate the host cell...and mediate efficient viral replication. More information on these viral genes is available in the adenovirus...

  16. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...products for packaging (Gag, Pol, and Env), the isoforms of these proteins, as well as the viral LTRs, ...different viruses and thus use slightly different isoforms of these packaging components. Therefore, lentiviruses...

  17. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    Published
    Jan. 21, 2025, 2:15 p.m.
    ...underutilized team potential. I often thought that performing a cell culture experiment myself would take less... Kim Scott Courses: Leadership programs on platforms like Coursera, LinkedIn Learning, or edX. Podcasts...

  18. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ... host cells (the simplest example would be a transformed mesophilic, for example E. coli, carrying a plasmid...2019 All-India iGEM Meet-up, which served as a platform for all the Indian iGEM teams to interact with...

  19. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ...How can I tell if my enzyme will cut? Whether performing a digest for cloning purposes or for diagnostics...consult the REBASE database for more detailed information. Enzyme Dam methylation Dcm methylation EcoKI...

  20. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ... and the nonmethylated PCR products are then transformed into E. coli to undergo nick repair. This method...template plasmid, these PCR products are directly transformed into bacteria expressing recombineering proteins...
Showing: 541 - 560 of 756 results