We narrowed to 773 results for: ire;

1. From Lab Bench to Leadership: Transitioning into Management as a PhD

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   Blog Post

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   Jan. 21, 2025, 2:15 p.m.

   ... goals, and fostering team success. This shift requires deliberate preparation and a willingness to embrace... members thrived on independence, while others required more guidance and structure. I quickly learned...

2. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   Blog Post

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   June 6, 2019, 12:41 p.m.

   ...limited deep-tissue performance, and sometimes require an alternative such as a radioactive tracer. As...cell differentiation. Furthermore, TALEN and ZFN require custom nucleases for each gene of interest. CRISPR-Cas9...

3. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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   Blog Post

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   July 28, 2017, 1:30 p.m.

   ...same chromosome. Targeted Insertion allows for direct comparisons between lines expressing different ...wondering, “How do I know which of my flies have the desired integration?” Not to worry! In flies, phenotypic...

4. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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   Blog Post

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   May 11, 2017, 2:30 p.m.

   ... contributed  by Jesse S. Boehm, the Associate Director of the Cancer Program at the Broad Institute of...Jess S. Boehm. Jesse S. Boehm is the Associate Director of the Cancer Program at the Broad Institute of...

5. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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   Blog Post

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   May 3, 2018, 1:48 p.m.

   ...thus HEPN-independent. These enzymes also do not require a protospacer flanking sequence, so you can target...or processed gRNA array (22 nt spacer with 30 nt direct repeat) mediating potent knockdown. Multiplexing...

6. Viral Vectors 101: Inverted Terminal Repeats

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   Blog Post

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   Oct. 19, 2023, 1:15 p.m.

   ...ITRs serve as the origin of replication and are required for replication, packaging, and vector persistence...usually an issue as they are thought to be self-repaired during the AAV replication process (Chen et al...

7. Sharing is Caring: But How to Distribute Open Hardware?

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   Blog Post

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   Feb. 28, 2023, 2:15 p.m.

   ...clarified, especially with regard to licensing models. Direct sharing of work from universities and research ...Deepak Krishnamurthy, Ethan Li, Pranav Vyas, Nibha Akireddy, Chew Chai, Manu Prakash, "Squid: Simplifying ...

8. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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   Blog Post

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   July 28, 2015, 2:30 p.m.

   ...antibodies to recognize and bind transcription factors directly, but that’s a slow-going process and it doesn’...transcription factors. The second vector is a homology directed repair donor plasmid with homology arms and a ...

9. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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   Blog Post

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   Feb. 24, 2015, 7:50 p.m.

   ...screens that can be done with the libraries will require access to next generation sequencing technology...due to the size of the backbone, transformations require electroporation. References & Further Reading...

10. Summer SciComm: Preprints

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    Blog Post

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    Aug. 16, 2022, 1:15 p.m.

    ...scientists can receive recognition for their efforts, inspire future research, and increase their impact. Open...’ comments, and author responses can be shared directly alongside the preprint while the work is still...

11. Antibodies 101: Affinity Reagents

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    Blog Post

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    April 14, 2022, 1:15 p.m.

    ...available for a large number of targets, and can require specific conjugates. And, of course, once you have...either re-develop or re-optimize your assay for an entirely new affinity reagent.    Fig. 1: A comparison...

12. Transferable Skills Guide: Problem Solving

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    Blog Post

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    Nov. 30, 2021, 2:15 p.m.

    ...and the lab, you may not be able to carry out the entire problem solving process on your own, but you can...skillset is a part of your toolkit is important. Paired with your other skills, both technical and transferable...

13. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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    Blog Post

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    Aug. 5, 2021, 12:15 p.m.

    ...BioRender.com. Image from Haoyi Qiu.   He Tian: Directed evolution of biosensors He Tian (goes by “Tian...developed a video-based high-throughput screen for directed evolution of biosensors based on their dynamic...

14. Seven Tips for Using LinkedIn as a Scientist

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    Blog Post

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    Oct. 1, 2019, 1:21 p.m.

    ...those planning a career in academia, it may not be required, but many academic scientists are starting to ...use LinkedIn messaging, not everyone likes to. A direct email can be the best way to get in touch if you...

15. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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    Blog Post

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    March 19, 2019, 1:31 p.m.

    ...pyogenes Cas9, dCas9-effector fusions, and xCas9, and requires no additional chemicals or modifications. This...optogenetic tools can be difficult because this requires detailed structural knowledge of the proteins....

16. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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    Blog Post

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    Feb. 28, 2019, 1:34 p.m.

    ...thinking about reproducibility and shared similar desires to take action. Thanks to Teytelman’s vast network...already run several reproducibility workshops as Director of Scientific Outreach at Code Ocean and through...

17. Human Germline Editing Using CRISPR

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    Blog Post

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    Aug. 10, 2017, 2:19 p.m.

    ...targeting efficiency of only 27.9% and homology-directed repair (HDR) taking place in 41.2% of edited cells...NHEJ rather than HDR. Figure 1: Homology-directed repair in targeted zygotes proceeds from the maternal...

18. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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    Blog Post

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    June 15, 2017, 2:30 p.m.

    ...peptide derived from cytochrome p450, CytERM, to direct the fluorescent protein to the Endoplasmic Reticulum...Plasmids for Subcellular Localization Browse the entire Fluorescent Protein Collection ...

19. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    Blog Post

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    Dec. 8, 2016, 3:30 p.m.

    ...of ccdB, leading to positive identification of desired clones.   ...Becomes a powerful cloning tool... efficiently, as those that do not contain the desired insert should not grow.   Figure 2: Image...

20. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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    Blog Post

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    April 22, 2015, 2:06 p.m.

    ...ability to turn on genes at different times. It requires more complex synthetic networks. Addgene: What... with the software to tell you which parts are required to get the function that you want. The last few...