We narrowed to 821 results for: LAS

...that the plasmid could be maintained in the absence of antibiotics, resulting in the plasmid pBAD-bARGSer-AxeTxe... 1248 ng of CMV-gvpA-IRES-BFP plasmid and 954 ng CMV-gvpNJKFGWV plasmid into 70%-confluent well of HEK... Fig. 1: Expressing bARGSer in E. coli. (a) Plasmid diagram of pBAD-bARGSer-AxeTxe. (b) Methods to ...overexpressed compared to accessory proteins. Excess gvpA plasmid added into the transfection mixture in HEK293T ...mammalian species to produce gas vesicles. New mARG plasmids Our new mARGs come as a set of two PiggyBac integratable...genes gvpNJKFGWV marked with Emerald GFP. These plasmids can be used directly in transient transfection... into the genome by cotransfecting them with a plasmid expressing PiggyBac transposase. In rtTA-cell lines...

....org Find Cre-Lox Plasmids Find Optogenetics Plasmids Find Chemogenetics Plasmids ...vectors expressing light inducible ion channels (plasmid # 20298 for example) have revolutionized Neuroscience.... These Cre/ FLP versions are retained in the cytoplasm until estrogen receptor ligand Tamoxifen is added...neurons in the living brain   Figure 1: Plasmid mix to label neuronal morphology (eYFP) and the...recombinase. After in-utero electroporation, this plasmid mix results in sparse labeling of cortical neurons...labeling of individual neurons one could reduce the plasmids amounts to reduce transfection efficiency. However...visualized at the same time from two different plasmids this approach becomes problematic as it reduces...

...these plasmids from Addgene. This publication then resulted in the development of a new plasmid, pCMV14...continue having access to plasmids during the COVID-19 pandemic. Over 7,000 plasmids for COVID-19 research...work to share plasmids to all scientists Resources on Addgene.org Find COVID-19 plasmids at Addgene Browse...Biological repositories that distribute mouse strains, plasmids, cell lines, and much more have been critical ...science is truly a global endeavor. Case study 2: Plasmids Another example of reagents that have broad applications...requested from Addgene. Figure 2: Total COVID-19 plasmids requested from Addgene from March 23, 2020 to ...to July 27, 2020. Browse Addgene's COVID-19 plasmids and resources! Case study 3: Mouse strains Reagents...

... After all, students build circuits in physics class to learn about electronics or mix chemicals together...everyone safe. None of this is conducive to a classroom setting, especially for a school with a limited... activities making their way into high school classrooms, like gel electrophoresis to separate DNA or ...translation. Unfortunately, having live cells in a classroom presents equipment needs, such as shakers and ...FD-CF pellets are easy for students to use in the classroom because the students simply need to add water ...developed the BioBits kit to be used in high school classrooms, but it could be widely adapted and used in a...transition your undergraduate students from the classroom to the lab. You could give your undergrads hands-on...

...Collection page, you can browse for plasmids by species and find our guides, plasmid kits and other resources for...microbial research. Kits, guides, plasmids, and more We have Plasmid Kits for microbial work with fluorescent... To make it easier for you to find plasmids for your work, plasmids in the Microbiology Collection are... a useful plasmid that’s not in the collection? Tell us about it with our Suggest a Plasmid Form! Finally...us at [email protected]. If you are looking for plasmids used in E. coli and yeast, you’re in luck! Addgene...develop resources for your community! To contribute plasmids made in your lab, see www.addgene.org/deposit ...

...transfection of single plasmids vs pooled plasmids; for multiple edits, single plasmids are generally more...transfected on a single plasmid. This is versus 2.4% of cells transfected with a pool of plasmids that contained...sites. This eliminates bulk from crRNA expression plasmids resulting in smaller, more streamlined vectors...contained one crRNA per plasmid plus a Cpf1 expression vector.  See the graph in figure 2 for a comparison of...of a crRNA array that can be processed by Cpf1. Lastly, an AAV vector was used to multiplex edit 3-4 genes...your research? Check out the Cpf1 multiplexing plasmids available from Addgene. If you have any questions.... Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell,163(3), 759-771. PubMed...

... expressed from lentiCas9-Blast. Purpose of LentiCas9-blast LentiCas9-blast is a Cas9 expression vector... express Cas9 and a gRNA from the same plasmid. LentiCas9-blast virus can be used to generate Cas9-expressing... (drumroll please)... Top Lentivirus: lentiCas9-Blast Most of Addgene’s lentiviral inventory is based ...been engineered to perform unique editing tasks, classic Cas9 nuclease, which introduces double stranded...lentiviruses. This means that if you received lentiCas9-blast lentivirus from Addgene, you could use it in conjunction...own lentivirus. Secondly, you can use a LentiCas9-Blast viral prep to create a single stable Cas9-expressing...with a single-vector system). Tips for using this plasmid Cas9 expression is typically cytotoxic, which makes...

... awarded plasmids from Addgene as a successful applicant to the Addgene, Seeding Labs Plasmid Grant. For...Learn about the Addgene, Seeding Labs Plasmid Grant Read about Plasmid Grant Recipient, Gladys Kaba Resources... fellow nonprofit, Seeding Labs, to distribute plasmids to researchers in developing regions and thereby...accelerate their research. Kwabena came across the plasmid grant in Seeding Lab’s Newsletter and recently ...plans to use his research experiences as well as plasmids from Addgene to bring new opportunities to Ghanaian... Kwabena applied for the Addgene, Seeding Labs plasmid grant. 7:12 - 7:11 : Kwabena's work in Christopher... recent recipient of the Addgene, Seeding Labs Plasmid grant and is working on a variety of projects in...

...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools... drug inducible Cas9 plasmids E. coli genome-wide inhibition library In the last edition of “What’s New...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...vector sequence. Homology arms that flank the donor plasmid cargo are complementary to either side of the double-stranded...domain needed for DNA unwinding. They’ve deposited plasmids for active and inactive CasX for E. coli and human...expression. Read the paper in Nature Find the CasX plasmids Drug inducible transcriptional activation and...estrogen receptor. This protein fusion stays in the cytoplasm, but with the addition of 4-hydroxytamoxifen, ...

...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...is inaccessible and the complex remains in the cytoplasm. Read the paper in Cellular and Molecular Bioengineering...Bioengineering Find the optogenetic repressor plasmids Cytosine base editors with minimized off-target...paper in Nature Biotechnology Find the base editor plasmids CRISPRi in Caulobacter crescentus Figure...once. Read the paper in mBio Find the CRISPRi plasmids A multiplexed gRNA piggyBac transposon system... Read the paper in Scientific Reports Find the plasmids Enhanced CRISPR-based DNA demethylation ...