Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 816 results for: LAS

Showing: 281 - 290 of 816 results

  1. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...these plasmids from Addgene. This publication then resulted in the development of a new plasmid, pCMV14...continue having access to plasmids during the COVID-19 pandemic. Over 7,000 plasmids for COVID-19 research...work to share plasmids to all scientists Resources on Addgene.org Find COVID-19 plasmids at Addgene Browse...Biological repositories that distribute mouse strains, plasmids, cell lines, and much more have been critical ...science is truly a global endeavor. Case study 2: Plasmids Another example of reagents that have broad applications...requested from Addgene. Figure 2: Total COVID-19 plasmids requested from Addgene from March 23, 2020 to ...to July 27, 2020. Browse Addgene's COVID-19 plasmids and resources! Case study 3: Mouse strains Reagents...

  2. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ... After all, students build circuits in physics class to learn about electronics or mix chemicals together...everyone safe. None of this is conducive to a classroom setting, especially for a school with a limited... activities making their way into high school classrooms, like gel electrophoresis to separate DNA or ...translation. Unfortunately, having live cells in a classroom presents equipment needs, such as shakers and ...FD-CF pellets are easy for students to use in the classroom because the students simply need to add water ...developed the BioBits kit to be used in high school classrooms, but it could be widely adapted and used in a...transition your undergraduate students from the classroom to the lab. You could give your undergrads hands-on...

  3. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    Published
    June 13, 2016, 7:08 p.m.
    ...Collection page, you can browse for plasmids by species and find our guides, plasmid kits and other resources for...microbial research. Kits, guides, plasmids, and more We have Plasmid Kits for microbial work with fluorescent... To make it easier for you to find plasmids for your work, plasmids in the Microbiology Collection are... a useful plasmid that’s not in the collection? Tell us about it with our Suggest a Plasmid Form! Finally...us at [email protected]. If you are looking for plasmids used in E. coli and yeast, you’re in luck! Addgene...develop resources for your community! To contribute plasmids made in your lab, see www.addgene.org/deposit ...

  4. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...transfection of single plasmids vs pooled plasmids; for multiple edits, single plasmids are generally more...transfected on a single plasmid. This is versus 2.4% of cells transfected with a pool of plasmids that contained...sites. This eliminates bulk from crRNA expression plasmids resulting in smaller, more streamlined vectors...contained one crRNA per plasmid plus a Cpf1 expression vector.  See the graph in figure 2 for a comparison of...of a crRNA array that can be processed by Cpf1. Lastly, an AAV vector was used to multiplex edit 3-4 genes...your research? Check out the Cpf1 multiplexing plasmids available from Addgene. If you have any questions.... Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell,163(3), 759-771. PubMed...

  5. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ... expressed from lentiCas9-Blast. Purpose of LentiCas9-blast LentiCas9-blast is a Cas9 expression vector... express Cas9 and a gRNA from the same plasmid. LentiCas9-blast virus can be used to generate Cas9-expressing... (drumroll please)... Top Lentivirus: lentiCas9-Blast Most of Addgene’s lentiviral inventory is based ...been engineered to perform unique editing tasks, classic Cas9 nuclease, which introduces double stranded...lentiviruses. This means that if you received lentiCas9-blast lentivirus from Addgene, you could use it in conjunction...own lentivirus. Secondly, you can use a LentiCas9-Blast viral prep to create a single stable Cas9-expressing...with a single-vector system). Tips for using this plasmid Cas9 expression is typically cytotoxic, which makes...

  6. Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana

    Type
    Blog Post
    Published
    Dec. 20, 2016, 3:30 p.m.
    ... awarded plasmids from Addgene as a successful applicant to the Addgene, Seeding Labs Plasmid Grant. For...Learn about the Addgene, Seeding Labs Plasmid Grant Read about Plasmid Grant Recipient, Gladys Kaba Resources... fellow nonprofit, Seeding Labs, to distribute plasmids to researchers in developing regions and thereby...accelerate their research. Kwabena came across the plasmid grant in Seeding Lab’s Newsletter and recently ...plans to use his research experiences as well as plasmids from Addgene to bring new opportunities to Ghanaian... Kwabena applied for the Addgene, Seeding Labs plasmid grant. 7:12 - 7:11 : Kwabena's work in Christopher... recent recipient of the Addgene, Seeding Labs Plasmid grant and is working on a variety of projects in...

  7. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...Turquoise (CeNL; Plasmid #85199), Yellow (YeNL; Plasmid #85201), Orange (OeNL; plasmid #85202) and Red ... Researchers have been sharing plasmids ever since there were plasmids to share. Back when I was in the...specific strategies and tactics for getting the plasmids he wanted. Addgene makes it so much easier to ...kDa. An example is the CRISPaint-NLuc construct (Plasmid #67178) for use in the system outlined in Schmid-Burgk...CyOFP1-NLuc-CyOFP1; Antares). Direct injection of plasmids for Antares or firefly luciferase into mouse livers...Red (ReNL; plasmid #85203) NLuc fusions. The Nagai lab has additionally deposited more than 25 fusions of.... This construct is available through Addgene (Plasmid #83926). Live-Cell Voltage Sensor  Ingaki and ...

  8. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...is inaccessible and the complex remains in the cytoplasm. Read the paper in Cellular and Molecular Bioengineering...Bioengineering Find the optogenetic repressor plasmids Cytosine base editors with minimized off-target...paper in Nature Biotechnology Find the base editor plasmids CRISPRi in Caulobacter crescentus Figure...once. Read the paper in mBio Find the CRISPRi plasmids A multiplexed gRNA piggyBac transposon system... Read the paper in Scientific Reports Find the plasmids Enhanced CRISPR-based DNA demethylation ...

  9. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools... drug inducible Cas9 plasmids E. coli genome-wide inhibition library In the last edition of “What’s New...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...vector sequence. Homology arms that flank the donor plasmid cargo are complementary to either side of the double-stranded...domain needed for DNA unwinding. They’ve deposited plasmids for active and inactive CasX for E. coli and human...expression. Read the paper in Nature Find the CasX plasmids Drug inducible transcriptional activation and...estrogen receptor. This protein fusion stays in the cytoplasm, but with the addition of 4-hydroxytamoxifen, ...

  10. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    Published
    July 1, 2025, 1:15 p.m.
    ...Best Way to Elute and Store Your Plasmid DNA? Selecting Your Plasmid Purification Kit Resources on Addgene.org...You have just purified your plasmid and are ready to move on to your downstream application — but wait...wait! Do you know how much plasmid DNA you have or how pure your sample really is?  To assess the concentration...concentration and purity of nucleic acids, such as plasmids, the go-to method is to use spectrophotometry....Protein contaminants Whether you are purifying plasmid DNA from bacteria or cleaning up a PCR product,... 230 nm is guanidine thiocyanate (used in many plasmid purification kits), which can dramatically reduce...assay or running an agarose gel. In the case of plasmids, the use of RNase treatment to degrade bacterial...
Showing: 281 - 290 of 816 results