We narrowed to 855 results for: NTS

...Thankfully, there is a tool to measure these electrical events! Voltage indicators are voltmeters for cells! In...chemigenetic sensors. These tools have two main elements: a voltage-sensing component and a fluorescent...voltage sensor with a genetic component. The VSD components have historically come from a variety of sources...squirts. This design structurally separates the two components of the indicator, which can make the kinetics...types of sensors can have a variety of different components, but all contain a FRET pair, a genetic component...Viral vectors are mechanisms by which genetic components can be delivered to cells both transiently and...covered. Happy voltage sensing!   Figure 3: Components of the types of voltage indicators reviewed. ...

...are used to repeating experiments to confirm their findings. But no scientist wants to repeat an experiment...errors increases. In addition to human error, fragments of DNA from the helper plasmids or the cell genome...serotype of the packaged DNA and look for potential contaminants. The analysis is a 2-step process using Geneious...software looks for and quantifies the presence of recombinants in recombinase-dependent sequences. Recombinase-dependent...powerful tool that can be used to identify DNA contaminants in AAV preparations and provide information ... AAV vectors for your research. Please use the comments section below to let us know if you have any specific...

...vectors in the brain and peripheral nervous system presents a special challenge to scientists. What works ...neurons (Figure 1). However, this one set of experiments didn’t provide any information on other important... of the virus to adjacent areas impacted the experiments we wanted to perform. It required a great deal...community concerns AAV vectors are complex biological reagents; many factors, like storage buffers and conditions...conducted to improve our Addgene Data Hub, 76% of respondents indicated they wanted to see more negative data...data they cannot explain, perhaps out of fear it points to flaws or gaps in their technical ability. But...and use this data to guide them in their own experiments. By providing DOIs, this information can be cited...

...lentiviral vectors! Lentiviral vector history and components In the early 1990s, researchers developed viral...and Pol, increasing the number of recombination events necessary to create a replication-competent virus...trial with a lentiviral vector. CD4+ T-cells from patients with HIV-1 were transduced with a lentiviral vector...sequence against the HIV-1 envelope. As of 2013, 65 patients had been treated with this therapy, which lowered...beta-thalassemia. Lentiviral vectors are promising agents for cancer immunotherapy, but this research is ... vectors contain a deletion in the 3’ LTR that prevents aberrant activation of nearby genes. These safer...experiment. Let us know how you use lentivirus in the comments section below! References 1. In vivo gene delivery...

... coli strains producing different pigments, and tasked the students to separate out colonies from each...second quadrant. The process continues until all quadrants are used, hopefully revealing single, isolated...incubation. The challenge is usually successful for the students. “They always get a few isolated colonies. I should...chromoprotein-expressing strains are easily amenable to other experiments to teach other aspects of molecular biology. ... do with the plasmids from Addgene is have the students transform them into different strains of E. coli...

...are going to be there – undergraduate students, rotation students, joining a new lab at any career stage... help clean up and/or make necessary reagents for the experiments. Unlike in lab courses, where everything...double-check you remember where the equipment, reagents, and samples are. Do: Ask your bench mentor if...everything is magically made up by helpful TAs or assistants, and sitting out on the bench waiting for you...with…now”. Ask for feedback so you can make adjustments. This could look like “What are some things I...

...minutes. While users can’t tangibly see these improvements, you are benefited with reassurance your plasmids...on stories during short periods of time called sprints. The team is mindful of the number of stories we...without impacting the overall work. Each of our sprints is themed and the stories in that sprint relate.... Third party libraries are reusable software components that can be incorporated into software. You can... can think of these libraries as ingredients to a recipe. It’s unrealistic to plant, grow, and harvest...cake depends on how you combine it with other ingredients.  For example, we use a third party library to...over again for every single software today that prints labels. These libraries in turn need to stay up...

...option, try the Biospace career events page. Biospace holds career events all over the country; sometimes... thereof) One of the biggest challenges facing students today is that many graduate and post-doctoral ... development training. Further, even for those students and researchers who do take the initiative to ...obviously important experience, but far too many students and young professionals will never attend a job...which are larger meetings offering resources for students seeking employment within industry or academia...using ResearchGate to see where other graduate students and post-docs from your institution have gone,...

...feeling out of their lab, but for many graduate students and postdocs, the people you see in your bay for... a lab. You formulate hypotheses and conduct experiments. You transfer liquids between tubes, look through...office transition? I’ve already provided a few counterpoints to some of the causes of culture shock above...it’s like to be properly onboarded. Most work environments outside of academia provide a formal training...probably because you don't have to worry about experiments taking longer than expected or dealing with unexpected... bring real scientists into schools to provide students with hands-on lab opportunities. You can get your...are there once you start looking for them. Acknowledgements Big thank you to Jessica Welch, Mike Lemieux...

.... This enhanced movement is thought to reflect elements of drug addiction and craving (‘seeking drugs’...approach against drug addiction. Anxiety tests Rodents have a natural preference to stay in dark areas...ones (risky). They also prefer to explore novel environments by initially moving along a wall (protected)...Taking advantage of these characteristic traits, rodents can be used to study anxiety levels in general,... driven by their motivation to explore novel environments, rather than being inhibited by fear/anxiety...test the efficacy of antidepressant treatment in rodents. In this test, an animal is placed into a tank ... of microRNA124a in the hippocampus evokes antidepressants-like effects in rats." Psychoneuroendocrinology...