We narrowed to 599 results for: REV;
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TypeBlog PostPublishedMarch 2, 2023, 2:15 p.m....post-purification cleavage step to remove the tag in order to prevent it interfering with protein function. In contrast...with the protein of interest (Bornhorst 2000). To prevent this, wash steps often include low concentrations...
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CRISPRainbow and Genome Visualization
TypeBlog PostPublishedFeb. 28, 2017, 3:30 p.m....calculated fluorescent distances correlating with the previously established physical map. In comparing pairs ...induce genome editing. Such a system has been previously described for transcriptional activation/repression... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog PostPublishedMay 11, 2021, 1:15 p.m....The pre-embedding immungold technique was used to reveal the hM4Di coupled to mCherry (top) or HA tag (bottom...RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method. Mol Cell... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog PostPublishedApril 23, 2018, 2 p.m....combines these approaches to enable rapid and reversible recruitment of chromatin complexes to any genomic...regulation and subsequent mathematical modeling. Its reversibility allows precise and quantitative analysis of ... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog PostPublishedMarch 19, 2019, 1:31 p.m.... the photoswitchable protein podcast segment Reversible control of photoswitchable proteins, which change...study, the Woolley lab showed that these photo-reversible protein-protein interactions can control subcellular... -
Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog PostPublishedFeb. 28, 2019, 1:34 p.m....Berkeley at the time and an eLife ambassador, had previous experience organizing open access events and grassroots...Scientific Outreach at Code Ocean and through her previous work at Center for Open Science and Sense about... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog PostPublishedJan. 31, 2019, 1:32 p.m....studies (Martins et al., 2018 and Xing et al., 2010) revealed precisely that for breast cancer. What are tumor...malignant forms and noticed that myoepithelial cells prevented progression to malignancy, concluding that these... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog PostPublishedOct. 29, 2019, 2:52 p.m....interdisciplinary field of biology known as bioelectricity is revealing the important role of bioelectrical signaling ...gradients for control of growth and form." Annual review of biomedical engineering 19 (2017): 353-387. PubMed... -
Addgene's Tips for Plasmid Quality Control
TypeBlog PostPublishedJan. 14, 2016, 3:30 p.m...., see image above). We then select forward and reverse primers for each sample with the help of DNA-editing...prepared with the primer stocks for forward and reverse reactions, respectively, and then shipped along... -
Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9
TypeBlog PostPublishedDec. 16, 2015, 3:30 p.m....detecting genome-wide double strand breaks (BLESS), revealed that these mutants do not cause off-target effects...to their enhanced specificity. Additional work revealed that mutations in the mobile Rec3 domain facilitate...