We narrowed to 836 results for: Abo
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TypeBlog PostPublishedSept. 12, 2019, 1 p.m....brightness after ~100 action potentials. This is about twice as bright as GCaMP7f and GCaMP7s. See all...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...
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Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog PostPublishedSept. 3, 2019, 1:15 p.m.... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here. ...neurons. Earlier this year, the Hegemann lab and collaborators report their discovery of a new family of seven... -
New and Upcoming Viral Vectors - June 2019
TypeBlog PostPublishedJune 18, 2019, 1:15 p.m....vector name and serotype you’d like to be notified about. Controls Plasmid Serotype Name 37825 AAVrg...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and... -
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog PostPublishedJune 4, 2019, 1:02 p.m....sensor). In preliminary studies with the lab’s collaborators, they were able to use the serotonin sensor ...Pantoea agglomerans interaction. But the shape and metabolism of leaves can be changed by light and temperature... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog PostPublishedAug. 2, 2022, 1:15 p.m.... it can be used in a lab? Here we’ll be talking about the ins and outs of this classification system, ...The classification system is also useful in a laboratory setting. When designing a plasmid to be inserted... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog PostPublishedDec. 6, 2016, 3:30 p.m....conduct the screen. To help alleviate some of this labor for our users, we now provide the Brunello (targeting... hear from you if you have used or are thinking about using our viral services. Please send any questions... -
Management for Scientists: Delegating is Key
TypeBlog PostPublishedFeb. 20, 2015, 8:30 p.m....Medical School researcher Connie Cepko to learn about her management style. Recipes for delegation success...accountability? What types of issues do you want to know about right away? Provide adequate support. The manager... -
From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog PostPublishedJan. 21, 2025, 2:15 p.m....clarifying roles, I eased the tension and improved collaboration. The good news: It’s a journey, not a final...of postdocs focusing on molecular biology and metabolism. He now guides fellow PhDs in their careers with... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog PostPublishedAug. 20, 2024, 1:15 p.m....interest. Created with biorender.com. The plot above is for a smeary expression and is quite different.... Additionally, you can gate off the histograms above, allowing you to visualize your data as a dot plot... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog PostPublishedAug. 6, 2024, 1:15 p.m....packaging methods. The three components described above (envelope, packaging, and transfer) are supplied...gamma-retrovirus. Frequently asked questions about γ-retroviral vectors How do engineered γ-retroviruses... -
Viral Vectors 101: Biosensors
TypeBlog PostPublishedJuly 6, 2023, 1 p.m....component of a biosensor is the thing that you care about within your cellular or animal system. This ‘thing...for expression and downstream monitoring. Nervous about making a virus for your biosensor? Don’t be! We ... -
Viral Vectors 101: Viral Applications
TypeBlog PostPublishedApril 13, 2023, 1:15 p.m....Viruses as Biological Tools blog can help you learn about the different types of viruses and pick one for ...Addgene.org Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog PostPublishedFeb. 28, 2023, 2:15 p.m.... in science brings stronger networking across laboratory and disciplinary boundaries and can allow for... that are otherwise cut off from the process. About the author: Benedict Diederich received his PhD ... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog PostPublishedJuly 28, 2017, 1:30 p.m....immunofluorescent images like in this paper (2). Metabolic assays such as Seahorse can be employed to look...Additional Resources on the Addgene Blog Learn about Using Cre in Drosophila Read Other Genome Engineering... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog PostPublishedJune 1, 2017, 2:30 p.m.... single amino acid. Without further information about properties like photostability or aggregation tendency...Fluorescent Proteins for Multicolor Imaging Learn about Fluorescent Protein Biosensors Additional Resources... -
Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog PostPublishedMay 9, 2017, 2:12 p.m....targets compared to Cas9. Read on to learn more about Cpf1 multiplexing. For an in-depth review of Cpf1... Addgene. If you have any questions or thoughts about Cpf1 multiplexing, leave them in the comments below... -
Screening for Successful Genome Editing with Digital PCR
TypeBlog PostPublishedMarch 30, 2017, 2:30 p.m....advantages over mismatch nuclease assays that will be elaborated below. Digital PCR Digital PCR (dPCR) involves...type targets. Comparatively, detection limits of about 5% mutant targets have been reported for mismatch... -
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog PostPublishedJune 25, 2019, 12:54 p.m....blog to learn about making your own competent cells and our protocols page to learn about bacterial transformation... -
New and Upcoming Viral Vectors - May 2020
TypeBlog PostPublishedMay 28, 2020, 1:15 p.m....vector name and serotype you’d like to be notified about. Chemogenetics Plasmid Serotype Name 44362 ...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog PostPublishedDec. 6, 2018, 1:34 p.m.... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here. ...can take as long as several days. In 2015, the laboratory of long-time Addgene depositor Alice Ting used...