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  1. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...Molecular Biology. Jake Watson is a postdoctoral researcher in neurophysiology at the MRC Laboratory of Molecular...homologous recombination in vivo." Nucleic acids research 21.15 (1993): 3601. PubMed PMID: 8346047. PubMed...

  2. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...derivatives are a staple in the lab. GFP is used in research across a vast array of biological disciplines ...protein and facilitating its widespread use in research. Many other mutations have since been introduced...

  4. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ... 16 labs around the world and can be used by researchers to validate their qPCR assay (Lock et al., 2010...titrating AAV vectors." Medical science monitor basic research19 (2013): 187. PubMed PMID: 23828206. PubMed Central...

  5. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...work for biotechnology companies and help other researchers use the company’s products successfully. In technical...involve interacting with other departments such as research and manufacturing, quality assurance, product ...

  6. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...contributed by guest blogger, Jennifer Rivers, a research microbiologist specializing in bacteriology and... Rivers! Jennifer Rivers ScM, MLS(ASCP)CM is a research microbiologist specializing in bacteriology and...

  7. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...thought it would be a good idea. After a bit of research, we realized that the r/Science AMA series was...posts were about toxic advisors, fruitless job searches, and even despair at having chosen an academic...

  8. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...importance of bioinformatics at Addgene and how our search for solutions led us to a partnership with Seqera... programs to a uniform language.  The modern architecture of Nextflow included built-in support for containers...

  9. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...A recent survey of PhDs found that many researchers feel they lack formal training in a variety of transferable...'re helping to fill this gap by both helping researchers recognize their MANY transferable skills learned...

  10. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...pathogen more safely. Recently, many SARS-CoV-2 researchers began pseudotyping the SARS-CoV-2 spike protein...Improve Gene Modification of Hematopoietic Cells for Research and Gene Therapy. Viruses 12:1016 . https://doi.org...

  12. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...polyethyenimine transfection protocol. I also recommend searching PubMed for any publications associated with the...feel more confident incorporating them into your research. And, of course, if you have any questions, don...

  13. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...pathways and spontaneous mutations.  Here, the researchers were able to target multiple genes in M. abscessus...catalyze the luminescence reaction (Figure 5). The researchers used the reporter to visualize biologically relevant...

  14. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...target!) and use them in numerous fields including research and development, diagnostics, drug discovery, ...interfere with conjugates. Make sure to do your research before conjugating at the bench.   Indirect ELISAs...

  15. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...commentary on their application to neuroscience research. What is CRISPR-based activation? To summarize...immunohistochemistry, it is not amenable to FACS.  Thus, the researchers inserted human CD8, a gene that encodes a surface...

  16. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...→ office culture shock? Working in an academic research lab involves a few key features that can be difficult...community (and sometimes conduct their own independent research!). There are also organizations like Science from...

  17. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...Blogger Kristian Laursen! Kristian Laursen is a researcher at Cornell University.       References 1. Breslauer... for site-directed mutagenesis." Nucleic acids research (2014): gku1189. PubMed PMID: 25399421. PubMed...

  18. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ... generate full libraries of mutant proteins. Researchers from the Whitehead lab developed One pot saturation...saturation mutagenesis? How would you use it in your research? References 1. Natarajan, Chandrasekhar, et al...

  19. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...we decided to write this quick guide to help researchers choose the probe for their needs. You’ll find...biosensors, and optogenetic tools. Follow her recent research @daria_gift_shch.     References and resources...

  20. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ... COVID-19 pandemic. The Pandemic Pivot Back in March 2020, just four months after I started with Addgene...completely out of reach for Addgene.  So, onto research! I needed to design a system that would allow ...
Showing: 561 - 580 of 777 results