We narrowed to 611 results for: CaS

1. Antibodies 101: Producing Recombinant Antibodies

   Type

   Blog Post

   Published

   Aug. 13, 2024, 1:15 p.m.

   ...directly into your preferred buffer. If that’s the case, a buffer exchange can be performed using a desalting...

2. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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   Blog Post

   Published

   Aug. 30, 2022, 1:15 p.m.

   ...binding by tweaking those parameters, but in some cases your antibody may just not be suitable for use with...

3. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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   Blog Post

   Published

   Sept. 8, 2016, 2:30 p.m.

   ...fluorescence upon binding to its cognate protein. In some cases this property eliminates the need for removing excess...

4. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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   Blog Post

   Published

   Aug. 9, 2016, 2:30 p.m.

   ...regions and their reverse complements were parsed for spCas9 PAM sites (NGG) and then filtered based on two ...

5. Lentiviral Vector Uses and Overview

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   Blog Post

   Published

   May 19, 2016, 2:30 p.m.

   ...Screening Read about Genome-Wide Screening Using CRISPR/Cas9 Additional Resources on Addgene.org Check out ...

6. Plasmids 101: Protein tags

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   Blog Post

   Published

   Dec. 11, 2014, 4:26 p.m.

   ... blots, IP, co-IP, IF, flow -cytometry; can occassionally interfere with protein folding HBH HHHHHHAGKA...

7. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

   Type

   Blog Post

   Published

   Dec. 2, 2014, 7:23 p.m.

   ... series of retroviral vectors expressing tagged dCas9 and guide RNA. The retroviral enChIP system enables...

8. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

   Type

   Blog Post

   Published

   May 15, 2014, 3:33 p.m.

   ...used to study protein-protein interactions. In this case, two portions of EGFP are fused to the proteins ...

9. Deciphering the Mysteries of Behavior with Viral Vectors

   Type

   Blog Post

   Published

   Sept. 17, 2019, 12:40 p.m.

   ...harbouring a Cre-dependent IL-1 beta overexpression cassette. Expression of Cre in these neurons turns on overexpression...

10. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

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    Blog Post

    Published

    April 2, 2019, 1:44 p.m.

    ...both homeobox genes that are part of a genetic cascade that determines the cell fate of the dorsal mesoderm...

11. Lab to Office Culture Shock

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    Blog Post

    Published

    Feb. 5, 2019, 1:04 p.m.

    ...inevitably have downtime and plenty of it. That’s not the case at an office job. I’ve frequently told job candidates...

12. How to Write a Scientific Review Article

    Type

    Blog Post

    Published

    Feb. 16, 2017, 3:30 p.m.

    ...Finally, you can sound credible and cool when you casually mention “Haery et al., showed that MYC expression...

13. Plasmids 101: Gateway Cloning

    Type

    Blog Post

    Published

    Jan. 12, 2017, 3:30 p.m.

    ...product or plasmid with an attP donor vector. In this case, we would use PCR to add attB sites to either end...

14. Twenty Years of Sharing: Expanding Accessibility

    Type

    Blog Post

    Published

    Nov. 21, 2024, 10:47 p.m.

    ...Sinapse Biotecnologia 2022 Brazil   In some cases, the distributors broadly helped increase access...

15. Antibodies 101: The Four ELISAs and When to Use Them

    Type

    Blog Post

    Published

    July 25, 2024, 1:15 p.m.

    ...process can be laborious and expensive. In some cases, conjugation methods can interfere with binding,...

16. Tips for Writing a Good Cover Letter

    Type

    Blog Post

    Published

    May 21, 2024, 1:30 p.m.

    ... to three paragraphs. This is where you make the case for why you are a good candidate, explain how your...

17. Plasmids 101: Broad Host Range Plasmids

    Type

    Blog Post

    Published

    April 11, 2024, 1:15 p.m.

    ...incorporate interchangeable antibiotic resistance cassettes (Prior et al. 2010), inducible promoters with ...

18. Bioinformatics at Addgene

    Type

    Blog Post

    Published

    July 14, 2022, 1:15 p.m.

    ...Nextflow for bioinformatics pipelining In many use-cases, including ours at Addgene, the overall purpose ...

19. Antibodies 101: Reading a Flow Plot

    Type

    Blog Post

    Published

    Feb. 6, 2024, 3:13 p.m.

    ... by looking at the different populations. In the case of Figure 2, one population is B- and another two...

20. Hot Plasmids - November 2023

    Type

    Blog Post

    Published

    Nov. 14, 2023, 3:35 a.m.

    ...edits, such as long or highly structured templates. Cas9 variants PE6e–g often had even higher editing efficiencies...