We narrowed to 614 results for: CaS

...directly into your preferred buffer. If that’s the case, a buffer exchange can be performed using a desalting...

... blog Engineering the plant genome using CRISPR/Cas9 Using pSiM24 for simplifying plant genetic engineering...

... of new capsid variants, designing expression cassettes to regulate and optimize transgene levels, and...

...antibody (HCab), which are unique to camels, llamas, alpacas and other camelids. In terms of structure, HCabs...

... infect the cell type of interest, which, in the case of Chan et al, was neurons and astrocytes. Why ...

...fluorescence upon binding to its cognate protein. In some cases this property eliminates the need for removing excess...

...regions and their reverse complements were parsed for spCas9 PAM sites (NGG) and then filtered based on two ...

...Screening Read about Genome-Wide Screening Using CRISPR/Cas9 Additional Resources on Addgene.org Check out ...

... blots, IP, co-IP, IF, flow -cytometry; can occassionally interfere with protein folding HBH HHHHHHAGKA...

... series of retroviral vectors expressing tagged dCas9 and guide RNA. The retroviral enChIP system enables...

...used to study protein-protein interactions. In this case, two portions of EGFP are fused to the proteins ...

... by looking at the different populations. In the case of Figure 2, one population is B- and another two...

...edits, such as long or highly structured templates. Cas9 variants PE6e–g often had even higher editing efficiencies...

... contamination. To date, Addgene has never had a case of mycoplasma contamination. In the event of contamination...

...Nextflow for bioinformatics pipelining In many use-cases, including ours at Addgene, the overall purpose ...

...Sinapse Biotecnologia 2022 Brazil   In some cases, the distributors broadly helped increase access...

...process can be laborious and expensive. In some cases, conjugation methods can interfere with binding,...

... to three paragraphs. This is where you make the case for why you are a good candidate, explain how your...

...incorporate interchangeable antibiotic resistance cassettes (Prior et al. 2010), inducible promoters with ...

...harbouring a Cre-dependent IL-1 beta overexpression cassette. Expression of Cre in these neurons turns on overexpression...