...your research, be sure to consider how its features do and don’t support reproducibility as not all ELNs...

...Agrobacterium strains see Weigel and Glazebrook, 2002. Tips: Do a colony PCR on your agrobacterium: Getting a transformant...

...single molecules - something researchers often want to do at neuronal synapses for instance. Also these tags...

...share your protocols with other groups. What apps do you use to improve your lab productivity? As this...

... list is a great place to start, the tables above do not delve into the tissue or development-specific...

... regions communicate with each other and how they do it (i.e. where the signals come from and what implications...migrates through neurons where it can replicate and by doing so spread across several synaptic connections (Ugolini...

... extension can usually ensure that the 3’-base(s) do not form secondary structures. The introduction (...

...clinical grade hiPSCs, reprogramming methods that do not involve modification of the original genome by...

...saturation mutagenesis with the same protocol. What do you think about one pot saturation mutagenesis? How...

...camera’s image to my remote location, live. After doing a bit of looking, I found an inexpensive HDMI transmitter...

...less stable than viruses produced in cell lines that do not express these sugars. (Takeuchi et al., 1997)...

...allowing co-imaging. And because bioluminescent markers do not need to be excited by an external light source...

...we can tell, DnaA doesn’t need to hydrolyze ATP to do its job — although ATP does get hydrolyzed over time...

...machinery such as Rep or other initiator proteins, they do not require host proteins for replication and can...spp., Rhizobium spp., Rhodopseudomonas spp., Rhodospirillum spp., Shewanella spp., Thiobacillus spp., Xanthomonas...

...biorender.com.   Of course, this is quite easy to do on a plot generated in Biorender! While a helpful...

...science.1138389 Le TTH, Tran NT, Dao TML, Nguyen DD, Do HD, Ha TL, Kühn R, Nguyen TL, Rajewsky K, Chu VT ...off-target editing, and, for RNA editing, fine-tuning dosage so that enough wild-type MECP2 is made inside the...

...find a positive one. So if you have better things to do than clone picking, choose your guide RNAs wisely...

...caption courtesy of Challis et al., 2022. Where do they come from? Systemic capsids are typically derived...efficacy can vary depending on species. LY6A and LY6C1 do not have known homologs in NHPs, and capsids that...mouse strains). AAV-PHP.eC, AAV9-X1.1 and AAV1-X1 do not display strain-specific tropism and can be used...2023). High viral titer High vector genome (vg) doses are recommended for all systemic capsid applications... AAV-PHP.eB is typically administered at a total dose between 1 x 1011 and 5 x 1011 vg per animal (i.e...mL titer), while AAV-PHP.S is typically used at a dose between 3 x 1011 and 1 x 1012 vg*. Most systemic...capsids are easy to produce in high titer, but higher doses (>1 x 1012 vg total in mice) may increase the chances...

..., phosphomimetic mutations on the activation loop do not fully activate MEK1. In fact, replacing just ...

...comparison. These next-generation sequencing techniques do not typically rely on Sanger sequencing but instead...