We narrowed to 629 results for: Dos;

...Hoshino and Fujii, 2009). Other forms of enChIP, that do not use dCas9, require Zinc-finger or TAL proteins...

...your research, be sure to consider how its features do and don’t support reproducibility as not all ELNs...

...Agrobacterium strains see Weigel and Glazebrook, 2002. Tips: Do a colony PCR on your agrobacterium: Getting a transformant...

...single molecules - something researchers often want to do at neuronal synapses for instance. Also these tags...

...was actually engineered to target the CNS. How well do these AAVs transduce the CNS and PNS? Both plasmids...from. Additionally, AAV.PHP.eB was used at a lower dose than it’s parent AAV.PHP.B: 1x1011 vs 1x1012 viral...blue (RGB) fluorescent protein (4a-c). When high doses of this viral cocktail are injected, by chance lots...the two green neurons near the arrows. When lower doses of virus are used in order to decrease the number...AAV.PHP.eB capsid. Both viruses were IV injected at a dose of 1x1011 vg per mouse and the level of transduction...AAV.PHP.S capsid. Both viruses were IV injected at a dose of 1x1012 vg per mouse and the level of transduction...-off transactivator (tTA), is injected at a high dose to maximize the number of cells that are labeled...

...explore. I hope that this brief guide can help you do just that. Good luck, and always keep going. Appendix...

...become resistant to? CRISPR may provide a method for doing just that. While challenges remain in the delivery...

...virus (similar to lentiviral vectors) because they do not encode all of the necessary viral proteins. Minigenome...

...protein molecules fail to correctly fold? Misfolded FPs do not fluoresce. A dark population is not readily apparent...

... list is a great place to start, the tables above do not delve into the tissue or development-specific...

...to use the deaminated DNA strand as a template. To do so, they used a Cas nickase, instead of dCas9. The...editors often produce a mixed population of edits, ABEs do not display significant A to non-G conversion at ...

... regions communicate with each other and how they do it (i.e. where the signals come from and what implications...migrates through neurons where it can replicate and by doing so spread across several synaptic connections (Ugolini...

...biorender.com.   Of course, this is quite easy to do on a plot generated in Biorender! While a helpful...

...allowing co-imaging. And because bioluminescent markers do not need to be excited by an external light source...

...we can tell, DnaA doesn’t need to hydrolyze ATP to do its job — although ATP does get hydrolyzed over time...

...machinery such as Rep or other initiator proteins, they do not require host proteins for replication and can...spp., Rhizobium spp., Rhodopseudomonas spp., Rhodospirillum spp., Shewanella spp., Thiobacillus spp., Xanthomonas...

...find a positive one. So if you have better things to do than clone picking, choose your guide RNAs wisely...

...saturation mutagenesis with the same protocol. What do you think about one pot saturation mutagenesis? How...

...camera’s image to my remote location, live. After doing a bit of looking, I found an inexpensive HDMI transmitter...

...less stable than viruses produced in cell lines that do not express these sugars. (Takeuchi et al., 1997)...