Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
Type
Blog Post
Published
April 5, 2018, 12:42 p.m.
...insertions, like a fluorescent protein knock-in, suffer from low efficiency when a standard dsDNA repair... secondary structure. Today, multiple companies offer long single-stranded DNA production services or ...Quadros et al. attribute much of this variation to differences in guide RNA efficiency. For example, in a two-guide...Quadros for assistance with editing this blog post.
References
Yang, Hui, et al. "One-step generation of mice...