We narrowed to 616 results for: LIS

1. The AAVantages of AAV in CRISPR Screens

   Type

   Blog Post

   Published

   Feb. 4, 2025, 2:15 p.m.

   ..., M., Farouni, R., Lagler, S., Kalamakis, G., Lischetti, U., Jabaudon, D., & Platt, R. J. (2023). Transcriptional...

2. Common Injection Routes in Mice

   Type

   Blog Post

   Published

   Jan. 22, 2025, 9:42 p.m.

   ... for you — and the mice. While not an exhaustive list, these can include what’s being injected, the type...

3. Production of Virus in Insect Versus Mammalian Cells

   Type

   Blog Post

   Published

   Nov. 5, 2024, 8:32 p.m.

   ...Tompkins, G. J., & McCawley, P. (1977). The establishment of two cell lines from the insectspodoptera ...

4. Magnetic Control of Proteins: More than a Dream

   Type

   Blog Post

   Published

   Sept. 24, 2024, 1:15 p.m.

   ...range of topics. He embraces open science, self-publishing directly on his website (and often posting updates...

5. 3D Printing Meets CRISPR Cas9

   Type

   Blog Post

   Published

   Oct. 17, 2017, 12:55 p.m.

   ...could make a model of Cas9 based on the structure published by Martin Jinek’s group. We had already made an...

6. Addgene's Cancer Collection Pages Connect the Research Community

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   Blog Post

   Published

   Sept. 6, 2016, 2:30 p.m.

   ...gene of interest will direct the investigator to a list of all available Addgene plasmids he or she can ...

7. Plasmids 101: Optimizing Plasmid Yields

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   Blog Post

   Published

   May 26, 2016, 2:30 p.m.

   ...handy guide from OpenWetWare for a more extensive listing of common E. coli strains. Increase the volume ...

8. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

   Type

   Blog Post

   Published

   April 19, 2016, 2:30 p.m.

   ...dimeric FP could ruin your day (and experiment) are listed below. Solutions to avoid these all too common ...

9. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

   Type

   Blog Post

   Published

   May 22, 2015, 4:58 p.m.

   ...negligible level. One major reason we chose to publish the concept and likely capabilities of RNA-guided...

10. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type

    Blog Post

    Published

    June 12, 2014, 3:30 p.m.

    ...purification; the whole cloning process is so simplistic once you have your parts. That was the motivation...

11. Tips for Using BLAST to Verify Plasmids

    Type

    Blog Post

    Published

    May 29, 2014, 1:29 p.m.

    ...alignment tools available. Try these resources for lists of alternatives to BLAST: ExPASy - http://www.expasy.org...

12. Which Fluorescent Protein Should I Use?

    Type

    Blog Post

    Published

    May 20, 2014, 2:06 p.m.

    ...pH changes (e.g. pHluorin, pHTomato). Keep this list handy as you plan your next experiment or to hand...

13. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type

    Blog Post

    Published

    Dec. 7, 2020, 2:53 p.m.

    ...genome engineering in bacteria, alongside more established tools such as Cas9-recombineering, CRISPRa/i,...

14. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type

    Blog Post

    Published

    July 16, 2020, 1:15 p.m.

    ...embed_player" overrideable=False, type='scriptV4', hide_playlist=True, viral_sharing=False, embed_button=False...

15. Summer SciComm Series: Modes of Communication

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    Blog Post

    Published

    July 1, 2022, 1:15 p.m.

    ...answered the above questions, you can create your short list of options and then pick whichever is most accessible...

16. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type

    Blog Post

    Published

    Oct. 10, 2023, 1 p.m.

    ... toxic to dividing cells. The other antibiotics listed below affect continuous cellular processes (e.g...

17. DNA Purification Without a Kit

    Type

    Blog Post

    Published

    Feb. 11, 2020, 2:15 p.m.

    ...adapted from the 1979 Patterson Protocol. When published, the Patterson Protocol was shown to purify DNA...

18. Droplet Digital PCR for AAV Quantitation

    Type

    Blog Post

    Published

    Jan. 16, 2020, 2:18 p.m.

    ... of parameters influencing droplet rain in the Listeria monocytogenes prfA assay-reduction of ambiguous...

19. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type

    Blog Post

    Published

    Oct. 4, 2018, 12:44 p.m.

    ... selected and ranked based on the previously established sgRNA rules and  potential on- and off-target...

20. Plasmids 101: Colony PCR

    Type

    Blog Post

    Published

    May 12, 2016, 2:30 p.m.

    ...restriction enzyme digest; however colony PCR can accomplish the same thing in less time and for less money...