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We narrowed to 853 results for: NTS

Showing: 561 - 580 of 853 results

  1. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ...to reuse plasticware or think about conserving reagents and buffers.  However, I had very environmentally... using.  I started to do little things to save reagents and money.  This was the start of my interest ...ethidium bromide, less stop buffer and miniscule amounts of plasmid sample.  How satisfying?  With practice...

  2. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...RecA and RecBCD in bacteria. When two repeating elements love each other very much undergo recombination...sites, and other plasmids that contain repeating elements. Knowing how to amplify recombination-prone plasmids...save you the headache of troubleshooting failed experiments. Pick the right strain While common E. coli strains...

  3. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...in turn binds to calcineurin-dependent response elements (CDREs) and drives gene expression. Because calcineurin...high throughput screening, calcineurin dynamics measurements, or monitoring in vivo. To overcome these technical...iodine (PI), allowing for calcineurin activity measurements in stressed or aging cell populations. ...

  4. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...hooked up to invasive, cumbersome systems during experiments – neither ideal for the intrepid researcher. ...control – this is important later! Next, additional components are added - the ‘bells and whistles’ – including...on the other hand, was the wild west for fiber implants. Due to the soft nature of the intestines and ...

  5. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ...find details they need for planning their next experiments using a specific AAV tool. They can search the...tools. This word cloud (using wordart.com) represents contributed data on brain injection site from... a guide for designing your future injection experiments. Have your data or lab notebook handy?  Contact...

  6. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    Published
    June 17, 2021, 1:17 p.m.
    ...reverse, making it unsuitable for some types of experiments. (To get more information on AraC and other promoter... aureochrome1 (VfAu1), led to functional BLADE variants, too. The team tested different linkers between... can do all of these more spatially confined experiments as well as those requiring reversibility that...

  7. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ... Getting a lot of empty vector contaminants in your cloning experiments and tired of having to PCR many...the coming years as we stay on top of the many developments in science and the plasmids, technologies, and...

  8. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...of their luciferase assay, they concluded that components in the buffer used for protein purification must... expression would require multiple transduction events. For these reasons, iTOP is best suited to binary...small molecule NSDB and Na+ hypertonicity are key elements of iTOP. iTOP can be used to transduce Cas9 and...

  9. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    Published
    Dec. 10, 2019, 1:58 p.m.
    ...measured through their triglyceride or cholesterol contents (what is commonly called bad cholesterol). However... and localization in vivo. The LipoGlo system represents a new, intriguing approach to expand our understanding... and offers many advantages over traditional measurements such as patch clamp recordings. However, technical...

  10. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...relationships between microbes and insects, mammals, and plants, among others. I particularly enjoyed the emphasis... their paths cross with each other and with the events of the times."     - Aliyah Weinstein, Marketing...community know what your favorite book is in the comments! Additional resources on the Addgene blog Learn...

  11. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...Recombinases And more! Control AAV For many AAV experiments, we recommend validating the serotype in your...vectors to more serotypes. We currently package four variants of these biosensors, which are optimized for specific... should improve the specificity of influence measurements by reducing photostimulation of the axons and...

  13. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...cells (as demonstrated in different cellular environments such as retina or glioblastoma). They also found...classical purification protocol involving iodixanol gradients. The current purification protocols for isolating...decipher the mechanistic aspects behind these improvements. What have exosome AAVs achieved so far? Exo-AAVs...

  15. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...candidate transcript targets analyzed. CRISPR-Csm presents an attractive alternative for nuclear-RNA targeting...hfYFP structure, the group rationally designed GFP variants with similar capabilities. If you’re looking for... temperatures, and in the presence of oxidizing agents. Adapted from Fig. 2 Campbell et al, Nature Methods...

  16. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    Published
    June 1, 2021, 1:15 p.m.
    ...high-security facility needed to perform the experiments to demonstrate that this approach was useful ...diverse. Some of them are undergraduates or PhD students who are just starting their journey into science...stops these one-time favors from becoming starting points for long-lasting partnerships Join us! We are a...

  17. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...Darrell Kotton 2. Stem cell scientists care about patients As any grad student who has spent years studying...: Dr. Yamanaka talked about his clinical trial patients with advanced macular degeneration; Laurenz Studer...focused on translational research is encouraging. Patients are key to the research, but they are also the...

  18. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    Published
    Aug. 28, 2014, 3:34 p.m.
    ...) and are ready to dive into your functional experiments! Whether you’ve cloned the plasmid yourself or...determine the relative sizes of the resulting DNA fragments. Restriction digests are commonly used to confirm...longer to achieve good separation of larger DNA fragments. I hope these tips demonstrate that plasmid ...

  19. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ... and you’ll need to track it throughout your experiments.  Now that that’s out of the way, you can put... reaction to understand if any of your buffer components could affect the reaction. For instance, sodium... - antibodies that are a good match for your experiments, and avoid the awkwardness of getting the right...

  20. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...the N and C terminal segments respectively of a GFP dependent Cre. The two segments come together in the...transduction patterns empirically for their own experiments. To this end, we offer control AAV in smaller...
Showing: 561 - 580 of 853 results