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...rules) to some volunteers, who filmed with their phones. Test: I loved the end result, but it was clear....  Build: If you’ve ever watched any behind the scenes footage in the last several years, you can usually...

...need to keep paper records as well as electronic ones? It is important to know in which ways your data...functionalities such as: electronic signatures, electronic witnessing and audit trails. How does the ELN provider ...

...dissociate from the ligase and bind to exposed lysines on neighboring proteins. Thus to capture protein...monitor protein-protein interactions Split-BioID combines a protein fragment complementation (PCA) technique...

...development, extended-open integrins enable tissue morphogenesis, organ development, and the shaping of the extracellular...the scientists and scientific process behind the scenes to the new antibodies and research coming out of...

...constructs for targeting Golgi, ER, and endosome membranes) (Figure 4). The authors used SuperPLD to manipulate...illumination, substantial PA accumulates in the plasma membranes, indicated by the PA probe colocalizing with SuperPLDx30...

...talks for non-academic settings. Many of these guidelines will apply to any type of science presentation...supposed to be done. They will start looking at their phones. Speaking longer just makes you seem unprepared...

...a challenge. Enter Easi-CRISPR: a method that harnesses the power of ssDNA donor molecules for homology...mouse embryonic stem cells. After selecting for clones with the recombined product, you’d microinject ...

...skills, positive disposition and motivation, open mindedness, and the ability to accept feedback and adapt...receive immense personal gratification as they witness their student’s progress and increases in self ...

...PMC1933048. 16. Cheng, Changming, et al. "Electrochemiluminescent assay for detection of extremely rare mutations... probe DNA-immobilized magnetic beads and chemiluminescence detection." Analytical Sciences 29.7 (2013...

...Statistics – The basics Statistical analysis is what determines if there is statistical significance among data...There are many, many statistical tests beyond the ones detailed in this article. These are just some of...

.... Yu, J., et al., Induced pluripotent stem cell lines derived from human somatic cells. Science, 2007....duodenal homeobox gene 1 induces expression of insulin genes in liver and ameliorates streptozotocin-induced ...

...of prime editing tools. We’ll summarize the major ones in Addgene’s repository later in the post. Advantages...). Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases...

... knowledge to inform on the qualities of what determines good sgRNA design. Incidentally, the first software...Cas-OFFinder will suffice. Cas-OFFinder provides a bare-bones data dump of ALL strings in a genome using any IUPAC...

...grade DNA!     This is just the first of many milestones Addgene is poised to reach in the near future...

...this is it!)  Tuesday: Stats roundup on Twitter Wednesday: 2022 Blue Flame Depositors Hall of Fame  Thursday...

...Toolbox for quickly generating zebrafish transgenic lines to study neurodegenerative diseases. Listen to Our...

...focus on generating knockouts using CRISPR and Wednesday will highlight some Cas9 variants. Next, we’ll...

...words of welcome! Executive Director Chonnettia Jones and Board Member Joanna Brownstein were also on ...

...)Author: Jason PellettieriNew Acoustic Reporter Genes: Ultrasound Imaging of Gene ExpressionAuthors: Marjorie...

...Light Interval System, or FLIS, incorporates a bioluminescence protein into the chitin that can be activated...