We narrowed to 580 results for: SIM

1. CRISPR 101: Homology Directed Repair

   Type

   Blog Post

   Published

   Jan. 26, 2023, 2:15 p.m.

   ...vertically at the orange arrows). If resolved dissimilarly (e.g. one junction is resolved on the crossing...

2. Multiple Plasmids at a Low Price: Kits from Addgene

   Type

   Blog Post

   Published

   Sept. 14, 2016, 2:30 p.m.

   ...The Gomis-Ruth lab designed the pCri system to simplify the protein purification process by making it ...

3. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

   Type

   Blog Post

   Published

   May 13, 2025, 1:15 p.m.

   ... is distributed across multiple plasmids. For simplicity, we’ll use the second-generation system as an...

4. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

   Type

   Blog Post

   Published

   Sept. 5, 2017, 1:58 p.m.

   ...pathway. Accomplishing this usually involves simultaneously introducing a homologous DNA repair template...

5. Technologies Enabled by NanoLuc® Luciferase

   Type

   Blog Post

   Published

   Feb. 8, 2018, 12:17 p.m.

   ...brightness and improved maturation time compared to similar orange fluorescent proteins. In their work, the...

6. Plasmids 101: Dimers and Multimers

   Type

   Blog Post

   Published

   Jan. 10, 2023, 2:15 p.m.

   ...control when performing a diagnostic digest is a simple but effective method for detecting plasmid multimers...

7. Is this the right place for me? 8 tactics for choosing a lab

   Type

   Blog Post

   Published

   Oct. 2, 2018, 12:56 p.m.

   ...emotions and assumptions. I’m not just talking about simple things like “Which route should I take to get to...

8. Plasmids 101: Restriction Cloning

   Type

   Blog Post

   Published

   Feb. 18, 2016, 3:42 p.m.

   ...grow overnight cultures for DNA purification. The simplest purification you can do is a miniprep, but if ...

9. When Fidelity Matters: A frank discussion about ligase fidelity

   Type

   Blog Post

   Published

   March 6, 2018, 1:49 p.m.

   ...extended to multiplexed probe sets that allow the simultaneous interrogation of multiple potential SNP sites...

10. Transferable Skills Guide: Teamwork

    Type

    Blog Post

    Published

    Nov. 28, 2017, 2 p.m.

    ...of the phase of my life that had me listening to Simon & Garfunkel’s “I Am A Rock” on repeat in my bedroom...

11. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type

    Blog Post

    Published

    Oct. 8, 2020, 1:15 p.m.

    ...). Mycoplasmas are considered to be one of the simplest and smallest bacteria. The absence of a rigid ...

12. Live and Let Dye: Self-Labeling Protein Tags

    Type

    Blog Post

    Published

    April 16, 2024, 1:15 p.m.

    ...Wood, M. G., Learish, R., Ohana, R. F., Urh, M., Simpson, D., Mendez, J., Zimmerman, K., Otto, P., Vidugiris...

13. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type

    Blog Post

    Published

    March 7, 2017, 3:30 p.m.

    ...mismatches. To use this tool to detect a BRAF gene edit, simply PCR-amplify the BRAF locus from the edited cells...

14. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type

    Blog Post

    Published

    Dec. 15, 2016, 3:57 p.m.

    ...system once recombinant clones are generated.  A simple way to do this is to express lambda red genes from...

15. Antibodies 101: Antibody Engineering and Directed Evolution

    Type

    Blog Post

    Published

    Aug. 5, 2025, 1:15 p.m.

    ...Degenerate primers Using degenerate primers is similar to error-prone PCR. Degenerate primers are a group...

16. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type

    Blog Post

    Published

    March 21, 2024, 1:15 p.m.

    ...search for the perfect way to light up your science. Simon Moe is an Associate Product Marketing Manager at...

17. A Needle in a Base-Stack: Cas9 Structural Biology

    Type

    Blog Post

    Published

    June 4, 2024, 1:15 p.m.

    ... the HNH and RuvC domains, which are named for similar nuclease folds found in other proteins. The C-terminal...

18. How to Deposit Your Plasmids with Addgene

    Type

    Blog Post

    Published

    Feb. 27, 2024, 2:15 p.m.

    ...a peer-reviewed journal. To start this process, simply enter your preprint title or a general description...

19. Deep Dive: qPCR

    Type

    Blog Post

    Published

    Sept. 29, 2022, 1:15 p.m.

    ...analogous Wilcoxon tests have all been shown to give similar results, so you only need to pick your favorite...

20. CRISPR/Cas9 FAQs Answered!

    Type

    Blog Post

    Published

    March 13, 2014, 4:08 p.m.

    ...introduce cleavage of the target site with efficiency similar to using a single chimeric gRNA. At the same time...