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We narrowed to 813 results for: TIM

Showing: 561 - 580 of 813 results

  1. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...Once upon a time, not so long ago, spCas9 was the only Cas enzyme widely available and applied by researchers...better experimental fit due to this. But now, it’s time to cast aside those concerns and embrace a new resource...

  3. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ... of Cas9 expression between experiments and over time (as compared to separate transductions with a single-vector...(Gi)-mCherry When using this virus for the first time, the Roth Lab recommends verifying cre expression...

  4. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ... folder that you are currently working in) every time you use them, leave them in their own folder. Doing...Organized Learn about Software You Can Use to Save Time and Track Your Experiments Try out the CRISPR Software...

  5. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...have some homology to each other; ~17 bases is optimal. After being treated with DpnI to digest away the...-mutagenesis could save busy scientists a lot of time. Let us know how REPLACR-mutagenesis works in your...

  6. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...process: It is important to give your plates enough time for any intact β-galactosidase to be expressed and...is evenly distributed and allow sufficient drying time before use. Beware of false positives: Blue-white...

  7. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...In the short time since its development, CRISPR/Cas9 genome editing has been used to study the effect...transcript levels of PTG1 and PTG2 were 3 and 31 times higher, respectively. In rice protoplasts, Xie et...

  9. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...only one HRP-conjugated RANbody can be used at a time. Samples can be sequentially stained with two HRP...expensive Can only stain with one HRP-RANbody at a time GFP, RFP, H2A2B, Gelsolin Spaghetti Monster: ...

  10. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...advantage of this alternative is the possibility of real-time imaging. Robert Singer’s lab at Albert Einstein ...tag after pulse labeling of a determined period of time. Thus, only newly synthesized proteins of interest...

  11. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...isolating total RNA, this method requires a lot of time and depending on how many samples you may have, ...isopropanol precipitated. This method reduced the time it took to isolate RNA from 20+ hours to around ...

  12. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...has thus benefitted from countless rounds of optimization. Dozens of variants exist with unique cutting...and diversify PAM sequences of Cas12a to further optimize it for therapeutic and laboratory usage. Fast...

  13. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...usually 1-3 of the first 3 bps are modified) for optimal stability and efficacy.  Base modifications What...mechanism to turn cutting off or on at a specific time. Recent advances in gRNA modification have overcome...

  14. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...the activity or function of a single protein? Oftentimes, they rely on protein purification. In this strategy...to the metal ions once the protein is folded. Sometimes a tag placed on one end will be blocked during...

  15. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...have been harnessing the power of dsRNA for a long time, even before we knew about all the diverse in vitro...Endogenous sources of duplexed RNA DNA:RNA hybrids Sometimes ssRNA just needs a buddy, and even DNA will suffice...

  16. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...degradation. Additionally, electron microscopy analysis is time consuming. GFP-tagged Atg8p or LC3: ATG8 is part...4.9 - 9 SEP 488* nm 508 nm ~6.5 - 9 *Optimal sequential excitations wavelengths as determined...

  17. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...the threshold at which salaried workers receive overtime payment for working more than 40 hours per week...following feedback from postdocs and faculty (at time of writing, Brigham And Women's in Boston also just...

  18. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...“Fix the Knowledge” or “gendered innovations” stimulates excellence in science and technology by integrating... one thing, cells and cell lines degenerate over time—and sex can be hard to determine. To remedy this...

  19. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...incoming plasmids are sequenced at least twice, and sometimes more depending on the number of features we need...sequencing reactions. In the more complex cases, we sometimes have to design new primers to sequence the important...

  20. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ... workhorse protein even more useful! From FPs optimized for oxidizing environments to photoconvertible...sophisticated experimental manipulations, saving the time and money needed to develop new lines. Using nanobodies...
Showing: 561 - 580 of 813 results