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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 780 results for: ina

Showing: 561 - 580 of 780 results

  1. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ... DNA that you want to assemble together in a combination of different ways according to your cloning strategy... or PCR products from a gel. You will avoid contamination from other DNA fragments and you will remove...

  3. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...expressed. Pho8Δ60 localizes to the cytosol in an inactive form unless autophagy is induced. Then non-selective... Pho8 from its uncleaved to cleaved form is the final read out of this assay. This is also a quantitative...

  4. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ... (such as LTRs or ITRs), hairpins, or strong terminators. Detailed instructions on other factors to consider...when designing your primers can be found here. Finally, you will want to have a well-organized database...

  8. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...carrying through on the actions you committed to. Finally, hiring a paid coach will make you take the experience...sensitive to this and have coaching plans that are financially accessible. Sometimes only a few hours of coaching...

  9. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ... One way to triumph over your neighbors is to eliminate the competition, and Penicillium isn’t the only...exciting molecular technologies!  This post was originally written by Jessica Welch in January 2017 and ...

  10. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...Originally published May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology...immune system of bacteria. These Acr proteins were originally found in type I CRISPR-Cas systems.   Four years...

  12. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...conjugated to the enzyme horseradish peroxidase (HRP). Finally, the substrate of HRP is added to the wells. This... the amount of your protein of interest in the original sample. The competitive ELISA is best used for...

  13. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ... DNA, an editor targets DNA (via catalytically inactive dCas9) to modify chromatin. A fluorescent protein...Ward lab created a tool-kit to expand upon these original tools that allows for both spatiotemporal protein...

  14. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...participate in a science photo competition. Or nominate yourself as an educator for a short, animated ...Masterclass in science communication. The National Co-Ordinating Centre for Public Engagement (NCCPE) provides...

  15. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...enable us to measure these properties, and what fascinating experiments could push these tools even further... Förster Resonance Energy Transfer (FRET) was originally described by Theodor Förster in 1948 as a variation...

  16. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ... determine the best solutions. The event also culminated in a joint American Society for Biochemistry ...Officer Harvard University  The symposium will culminate with a keynote address by former congressman and...

  17. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...applications. Get More Cpf1 Background Here THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN ... What we still don't know about Cpf1 In their original paper characterizing Cpf1, Zetsche et al. suggested...

  18. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...affinity for specific cell and tissue types. The combination of serotypes and psuedotypes means you can customize...stably express Cas effectors in specific tissues, eliminating the need to deliver the Cas protein altogether...

  19. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...increase the enrichment of target-specific sybodies. Final enrichment via enzyme-linked immunosorbent assay...each sybody candidate. These tags are used in a final enrichment step for identifying sybodies that bind...

  20. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...exchange occurs through two sequential homologous recombination events: The plasmid carrying the desired genetic...activity. ShadowR can be used as a FRET acceptor in combination with mRuby2 or mScarlet as the donor in HeLa ...
Showing: 561 - 580 of 780 results