We narrowed to 855 results for: NTS

... cloning efficiency measurements. We performed additional efficiency measurements using similarly sized...number of transformants); the greater the number of inserts, the lower the number of transformants. To compensate... Table 1). These improvements (5- to 12-fold increases in the number of transformants while maintaining...reaction volumes were 20 μl (1, 12 fragments) or 25 μl (24 fragments). The future of assembly DNA assembly...Assembly is commonly used to assemble 2–10 DNA fragments in a single “one-pot” reaction to form complex... site is no longer present, allowing multiple fragments to be assembled in an ordered, pre-determined ...background by antibiotic selection. Screening of transformants by colony PCR confirmed the insertion of the...

...CRISPR applications in plants.     Transgenic yeast as source of dietary supplements Dr. Mrinalini Nair,...well as developing yeast as source for dietary supplements! Development of a bacterial expression system..., bacteria, and fungi that reside within living plants and that produce compounds with various antimicrobial...More Optogenetic Tools Like pDawn  Making cassava plants resistant to cassava mosaic virus Dr. Fiona Robertson...make use of the CRISPR/Cas9 technology to engineer plants that are able to destroy DNA viruses, similar to...interested in applying gene technology to bacteria and plants. Her research involves cloning cry genes from the...against local insect pests. By developing biological agents such as insecticidal proteins, the use of chemical...

...share these improvements? You could publish a paper that happens to include your improvements, or maybe ...the thousands of other scientists using pLKO.1 variants learn about those updates? This is a problem many...information, you’ll have the option to add additional documents. By uploading your protocols onto your plasmid... to your protocols when they begin their own experiments. Need to submit an updated protocol? Just email...them public for others to use, and add your own comments. Currently you can find protocols from NEB, various...storage and inventory, managing orders, tracking students and research projects, electronic lab notebooks...trials and pricing options for academic labs, departments, industry, and more to fit every lab's needs....

...responses in patients. They were able to tweak this assay to study SARS-CoV-2 spike variants. “We created...circulating variants. This allowed us to create pseudoviruses that represent lineages and variants that are...we’ve seen the emergence of several SARS-CoV-2 variants coincide with increased vaccination efforts. So...effective are the current vaccines against the variants? Alejandro Balazs, a Group Leader at the Ragon... of current vaccines differed depending on the variants (Garcia-Beltran et al., 2021). Antibodies from...Moderna or Pfizer vaccine could neutralize many variants, including the B.1.1.7 (Alpha variant), B1.1.298...well as they could for the wild-type. However, variants such as the P.1 (Gamma variant) and the B.1.351...

...Quantifying the abundance of thousands of protein variants simultaneously  With increasing sequencing power...individual’s genome. But when they find genetic variants, what does that mean? Previous research shows ...approximately 75% of all protein-coding pathogenic human variants are decreased in protein abundance, possibly due... due to misfolding. But measuring variants one at a time is time consuming and unscalable.  Melissa Chiasson...Washington sought to address the effects of genetic variants in a high throughput manner. She helped develop...a method that measures the effects of sequence variants on abundance simultaneously called VAMP-seq, for...protein variant library is monitored by fusing the variants to eGFP followed by an internal ribosomal entry...

... 20-nucleotide (nt) guide sequence that is complementary to the DNA target. The 20-nt guide region binds...the 20-nt Guide Sequence Identify a TAM site (5′-TTGAT-3′) in your target gene. Choose a 20-nt sequence...bacterial selection. The process for cloning the 20-nt guide RNA is identical for both vectors. Here, we...Dra2TnpB) to create a genome editing system for plants (Karmakar et al., 2024). This version of TnpB is...a different TAM (5′-TTAT-3′). The unique TAM requirements expand the number of genomic sites that can ...have a familiar targeting mechanism. Small RNA elements called right-end RNA (reRNA) or omega RNA (ωRNA...genome editing is the efficient delivery of editing reagents into cells. Viral vectors, because of their natural...

...and their research. These researchers span four continents and research diverse topics including microbiology... University of Ibadan. She works with medicinal plants such as Launaea taraxacifolia (Willd), an annual...renewable sensor, a bacterium that can sense contaminants and emit light as a response. To create this...fluorescent protein transcription in response to river contaminants and transform these plasmids into a bacterial...geographical locations. In addition to providing reagents and educational resources for scientists globally... Check out our CRISPR resources and CRISPR for plants Bacterial Expression Systems Synthetic Biology ...

... also shorter than those needed for Cas9 (42 nt vs 100 nt.) However, the newly described non-CRISPR protein...Additionally, whereas Cas9 generates blunt ends 3 nt upstream of the PAM site, Cpf1 cleaves in a staggered...smaller than any of these options (2.7 kb with 24 nt guides), so it has the potential to eclipse any of...into AAV when you add in a gRNA and regulatory elements. To solve this problem, the Zhang lab has previously...Kleinstiver et al., 2016. Until recently, two major elements of Cpf1 characterization were missing: on-target...

...mismatches in target RNA from bases 12-26 of a 30 nt target sequence. For the editing portion of the protein...sequencing, they confirmed A->I editing and found that 50 nt spacers increase editing efficiency but also increase...efficiency. They note that RESCUE is most active in a 30 nt guide when C or U base-flips are present across from...

...for a surprise! Whereas Cas9 generates blunt ends 3 nt upstream of the PAM site, Cpf1 cleaves in a staggered...doesn’t require a tracrRNA, crRNA guides are only ~42 nt long. Direct synthesis of these crRNAs should be ...be significantly cheaper than that of the ~100 nt crRNA/tracrRNA hybrid guides needed for Cas9 function...depletion assay to discover FnCpf1’s PAM sequence requirements. Cpf1’s preferred PAM is 5’-TTN, differing from...tracrRNA. Once they had determined the minimal elements of CRISPR-Cpf1, Zetsche et al. turned to characterizing...