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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 599 results for: REV;

Showing: 21 - 30 of 599 results

  1. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...of dsRNA, their recognition and consequences. Nat Rev Mol Cell Biol, 23: 286-301 (2022). doi.org/10.1038...pesticides and insecticides, an advancement over previous chemical versions with toxicity to many organisms...

  2. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...natural single-domain antibodies. Annu Rev Biochem. doi: 10.1146/annurev-biochem-063011-092449.            ...

  3. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...further split the viral genome by removing the viral Rev gene from the packaging plasmid and incorporating... lentiviral packaging systems were designed to prevent the formation of replication competent lentivirus...

  5. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...evolutionary classification of CRISPR-Cas systems. 5. Nat Rev Microbiol. 2015 Sep 28. doi: 10.1038/nrmicro3569....elements. To solve this problem, the Zhang lab has previously used SaCas9 (1053 aa/3.2 kb) with AAV vectors...orthologs show lower off-target activity than that previously observed with SpCas9. Kleinstiver et al. suggest... site due its proximity to the cleavage site, preventing future edits. In contrast, since Cpf1 cleaves...mediate HDR in a “second chance” mechanism. To prevent new editing post-HDR, repair templates should remove...Been KW, Yoon SH, Kim JS. Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells...

  6. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...applications in imaging living cells and tissues”  Physiol. Rev. 90:1103. Wu et. al. (2011) “Modern fluorescent proteins...Dendra2, and the mEOS proteins. Some proteins are reversibly switchable (e.g. rsEGFP, Dreiklang). Fluorescent...Plasmids 101: Green Fluorescent Protein (GFP) Three Review Articles on the Different Types of FPs: Stepanenko...

  7. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...contribution of synonymous mutations to human disease. Nat Rev Genet 12:683–691. https://doi.org/10.1038/nrg3051...across all endogenous genes in E. coli and S. cerevisiae (Tuller et. al., 2010). Figure 1: An overview...in Natural or synthetic? How Addgene;s dataset reveals trends in biological innovation Resources on Addgene.org...

  8. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...Therapeutic Purposes. Annu Rev Virol. 2014 Nov;1(1):427-51. DOI: 10.1146/annurev-virology-031413-085355. ... disease and not another genetic disease more prevalent or life-threatening (cystic fibrosis, hemophilia...manufacturing of viral vectors to support trials for prevalent diseases - to name a few. CRISPR-based tools ...hopefully only the beginning of a gene therapy revolution and the development of personalized medicine.... Vol 526, p.351. PubMed PMID: 26469046. 6. The Forever Fix: Gene Therapy and the Boy Who Saved It by Ricki... Retina. Front Cell Dev Biol. 2018 May 14;6:53. Review. PubMed PMID: 29868583. PubMed Central PMCID: PMC5960719...

  9. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...probes targeting integrated copies of the lentiviral Rev responsive element (RRE) gene and the cellular ribonuclease...titer (protocol modified from Lock et al., 2014.) Previous experience suggests that titers obtained using...

  10. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...evolutionary classification of CRISPR-Cas systems." Nat Rev Microbiol. (2015). PubMed PMID: 26411297. Resources...putative new class 2 nuclease, Cpf1 (CRISPR from Prevotella and Francisella), was annotated in several genomes...site due to its proximity to the cleavage site, preventing future edits. In contrast, since Cpf1 cleaves... has not yet been experimentally confirmed. To prevent new editing post-HDR, repair templates should remove...orthologs show lower off-target activity than that previously observed with SpCas9. Kleinstiver et al. suggest.... 3. Kim, Daesik, et al. Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells...
Showing: 21 - 30 of 599 results