.../genetics.115.176594 Yang H, Wang H, Shivalila CS, Cheng AW, Shi L, Jaenisch R (2013) One-Step Generation... find that many, if not all, the mice carry a frameshift mutation. For knock in with a donor oligonucleotide... genome. However, with only 3.4% of founders establishing germline transmission of the transgene, this...https://doi.org/10.1038/nbt.3026 Gurumurthy CB, Takahashi G, Wada K, Miura H, Sato M, Ohtsuka M (2016) GONAD...doi.org/10.1038/s41467-017-02706-7 Wang H, Yang H, Shivalila CS, Dawlaty MM, Cheng AW, Zhang F, Jaenisch R ...

... 2011. 767: p. 411-8. PubMed PMID: 21822892. 33. Shi, Y., P. Kirwan, and F.J. Livesey, Directed differentiation...thanks to our guest blogger, Kusumika (Kushi) Mukherjee. Kusumika (Kushi) Mukherjee is the Editor of Trends...This post was contributed by Kusumika (Kushi) Mukherjee. The ultimate goal in the field of regenerative... pursue a career in science communication and publishing after completing her postdoctoral training from...441(7097): p. 1061-7. PubMed PMID: 16810240. 2. Takahashi, K., et al., Induction of pluripotent stem cells... 131(5): p. 861-72. PubMed PMID: 18035408. 3. Takahashi, K. and S. Yamanaka, Induction of pluripotent ...

..., Liu, J., Zhang, H., Xie, L., Ying, W., Li, D., Shi, L., Sun, Y., Li, Y., Li, Y., Sun, Y., & Zuo, E. ...

...collaborations and partnerships. There are many different ways to maintain a relationship - many of them ...about the current state of a partnership. Every collaboration and partnership is different as are the individuals... the next level. External collaborations and partnerships are also very important in a non-research context...although we do that too), we will often look for partnerships where we can leverage another organization’s...’s expertise to our mutual benefit.  Our partnership with SnapGene, for example, allows us to display ...and now must focus on managing the established partnership. If you’re willing to do similar things for your...position at Addgene I am now involved in Strategic Partnership Management, a very interesting and exciting part...

...residence or citizenship to continue their work in the US have the added burden of needing sponsorship. If you...create therapeutics to cure the cancer. Authorship:  Publishing books, journals online and/or in print.... in the United States. There has been a growing shift away from employers sponsoring individuals for green... permanent residence case with United States Citizenship and Immigration Services (USCIS). If it were ...last year less than 30% of applicants received sponsorship) then he or she can start working in October ...can be a good choice for individuals needing sponsorship. Academic H1B The academic H-1B allows employers...National Interest Waiver (NIW) allows for self-sponsorship of the I-140 application meaning that the category...

... a variety of science policy roles including fellowships, volunteer positions, and part-time opportunities... give advice and guidance to those that ask. Relationship building is key in science policy. Luckily, ...happy hours that you can attend to help build relationships.  Reach out to a science policy professional... product or a well-endowed philanthropy who can shift the funding landscape to focus on a neglected disease...the organization, these changes to policy could shift how society interacts with certain scientific areas...compiled by the AAAS Science and Technology Policy Fellowship Program to learn more about where science policy...policy? Many science policy training programs and fellowships have a strong focus on early career researchers...

...urged you to work on developing your “leadership skills”. Leadership is prized at all levels of an organization...All three roles are important to accomplishing any goal or finishing any project. Doers are the ones who...Business Review 10 Must Reads: On Leadership (Articles on Leadership, also can be purchased individually...inundated with articles and books on the topic of leadership. Perhaps one of your advisors or mentors has ...   I am often asked to give career seminars on Leadership Skills. After attempting to put together such...what skills were really leader-specific. How is leadership different from good management? Aren't all career... doers, some become managers and some take on leadership roles. You may be happy with a primary role of...

... voltage will shift the population to the right while decreasing the voltage will shift it to the left... peak that shifts to the right on a histogram plot and lower for a signal peak that shifts to the left...103, while the GFP+ population cluster would be shifted to the right in comparison, say somewhere between...underwent different treatment processes, you may see a shift in the negative population, so it no longer overlaps...shows peaks of high signal densities, so signal shifts due to upregulation or downregulation are easier... plots For smeary expressions, you can quantify shifts in signal using the mean fluorescence intensity... the green (top) has an upregulated expression (shift to the right). The blue (third from top) could be...

...platforms.  As you can imagine, shipping to so many countries means that our shipping team is always engaged in... country that we can export to — so far, we’ve shipped materials from our repository to an amazing 111...contracted with so we can always offer the fastest shipping. However, there were issues that persisted despite...Table 2: Addgene distributors Distributor Partnership Start Date Country Served Beijing Zhongyuan...materials, we noticed a large percentage of these shipments arrived in India at room temperature. Partnering...distributor for that country. In China, direct shipments weren’t able to clear import control. Partnering...number of barriers. Though we haven’t solved every shipping issue for every country, we’re continuing to use...

...mentor. How do you make the most of this new relationship? Consider adding formality and active goal setting... setting to your mentoring relationships, so that you can reap rewards in the form of reaching career ... career path is a great start to a mentoring relationship, but if you don’t take this past the chatting...meetings and setting the direction in a mentoring relationship falls on the shoulders of the Mentee. A Mentor...progress over the course of an ongoing mentoring relationship (in a book or online). Mentee: Make a list of...secret of accountability To make a mentoring relationship really productive, the most important step you... PhDs with interesting careers, find a summer internship, etc.). It is helpful to work together on setting...