...-4  Swiech L, Heidenreich M, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F (2014) In vivo interrogation... Originally published Jul 14, 2015 and last updated Sep 16, 2020 by Beth Kenkel.  CRISPR genome editing...Cas12b and CasX. While there are no papers yet published that deliver these Cas proteins with AAV, at just...

...saturate the detector. In between these extremes lies the linear detection range. Figure 4: The...

...plasmids here! Zhong, B. L., Elliot, J. M., Wang, P., Li, H., Hall, R. N., Wang, B., Prakash, M., & Dunn, ...and in ternary complexes with other proteins, establishing CRBNmidi as a promising tool for future structure-guided...

...G., Chiu, A., Bader, A., Langan, E., Siebert, S., Li, J., Greiner, D., & Anderson, D. (2015). Neutrophil...strategy Figure 4 shows a gating strategy from a published experiment (Barlow-Anacker et al., 2017). This...

...Engineering Approaches with the CRISPR/Cas9 System. Li, K et al. PLoS ONE 9(8); (2014). PubMed. Making...

...academic research environment. The “how many?” question lies at the base of all these questions as there is not... career development. Publishing Panel The Early Career Researchers in Publishing Panel brought in editors...Outcomes University websites do not currently publish career outcomes for PhDs or postdocs. Having this...Path Panel and the Early Career Researchers in Publishing panel, focused on showing researchers that they... PIs and all gave advice on how to succeed in publishing in the current academic environment. Some great...writing skills will help you in your academic publishing goals and make you a better communicator overall...have any questions or concerns throughout the publishing process. The diversity panel - Equity and inclusion...

...luckily, the vast majority of my cells of interest lies within a clear negative population. When you’re ...

...PMID: 25514926. PubMed Central PMCID: PMC4383998. 3. Li, Tong, et al. "Cysteinyl‐tRNA formation: the last...difficulties with kinase specificity in vitro. The establishment of a system in which purified, recombinant protein...interested in the intersection of cell signaling, metabolism, and the mighty mitochondrion. You can follow...

...common diseases. References Zhang Y, Yin C, Zhang T, Li F, Yang W, Kaminski R, Fagan PR, Putatunda R, Young...viral reservoirs, and CRISPR may be the way to accomplish this tough job! Kamel Khalili’s lab at Temple...infected cells, an idea that became much more realistic with the advent of CRISPR. Using CRISPR/Cas9 SAM...enough T-cells could be reached to ablate a well-established viral reservoir. A second challenge for CRISPR...developing resistance. Shortly after Kaminski et al. published their results, Wang et al. showed that HIV-1 could...

...343-345. PubMed PMID: 19363495. 2. Wang JW, Wang A, Li K, Wang B, Jin S, Reiser M, Lockey RF. CRISPR/Cas9...

... . https://doi.org/10.1038/mt.2009.280 Hirsch ML, Li C, Bellon I, Yin C, Chavala S, Pryadkina M, Richard...Iodice C, Polishchuk EV, Sommella A, Colecchi L, Rossi S, Simonelli F, Giunti M, Bacci ML, Polishchuk RS, Auricchio...Marrocco E, Rossi S, Giunti M, Palfi A, Farrar GJ, Polishchuk R, Auricchio A (2013) Effective delivery of large...

...Cordell JL, Cragg MS, Šerbec VČ, Jones M, Lisnic VJ, Kremer L, Li D, Koch-Nolte F, Pascual N, Rodríguez-Barbosa...unless you share the results with others. If you publish data generated using your antibody, it’s best scientific...

...22205518. PubMed Central PMCID: PMC3574576. Lai, Sen-Li & Tzumin Lee. “Genetic mosaic with dual binary transcription...multiple genes at the same time.  The 3 binary systems listed above are orthogonal, meaning that they do not ...doi.org/10.1038/s41598-022-07852-7 De Boer, Herman A., Lisa K. Comstock & Mark Vasser. “The tac promoter: a ...

.... Biol., Apr. 2016. PubMed PMID: 27075976. 4.  X. Li, E. R. Burnight, A. L. Cooney, N. Malani, T. Brady...enhanced random integration efficiencies that are accomplished with transfections and are therefore virus-free...enhance gene editing? Gene editing can now be accomplished in a more cost-effective and efficient manner...team.     Mathew Goodwin is a Technical Sales Specialist at Transposagen. As a gene editing enthusiast...Singh, V. V Adjan Steffey, T. Yeshi, and D. W. Allison, “Gene Editing in Human Pluripotent Stem Cells:... Perry, V. V. A. Steffey, K. Miller, and D. W. Allison, “Decoding the Epigenetic Heterogeneity of Human...

...science.aae0169 2. Long C, Amoasii L, Mireault AA, McAnally JR, Li H, Sanchez-Ortiz E, Bhattacharyya S, Shelton JM, ... treat disease postnatally. Now, three papers published concurrently in Science have shown CRISPR can ...

... PMC2790811. 5. Thomason, L. C., Sawitzke, J. A., Li, X., Costantino, N. and Court, D. L. 2014. Recombineering...Central PMCID: PMC307639. 2. Daiguan Yu, Hilary M. Ellis, E-Chiang Lee, Nancy A. Jenkins, Neal G. Copeland...

...Papworth, G., Watkins, S., Trucco, M., Robbins, P. D., Li, J., & Xiao, X. (2006). Widespread and Stable Pancreatic...

... than APC. While APC's global excitation maximum lies around 650 nm, APC-Cy7's global excitation maximum...

...plasmids from this paper at Addgene. 6. Xing, Hui-Li, et al. “A CRISPR/Cas9 toolkit for multiplex genome...Originally published Jan 28, 2016 and last updated Sep 10, 2020 by Jennifer Tsang. CRISPR makes it easy...

...PDB ID 7S4X). The end of the DNA:RNA heteroduplex lies between the RuvC (orange) and HNH (red) domains,... will destabilize the nascent heteroduplex and abolish cleavage (Pattanayak et al., 2013). As mentioned...

...Wilson, Ning Sun, Deepak M. Gupta, Mei Huang, Zongjin Li, Nicholas J. Panetta, Zhi Ying Chen, Robert C. Robbins...reported in González et al. A percentage is not listed because many variables (cell type, criteria used...Check out the links at the end of the post for a list of Tet-inducible reprogramming vectors available...

... linker (see Figure 1). The key to the biosensor lies in pH: DsRed is relatively pH-insensitive, while...plasmids in a future blog post. No time to read? Listen to our hot plasmids segment on the Addgene Podcast...constructed. The authors then used GMAP assembly to establish a collection of over 30 promoters and 140 genes...characterize positive hits. This work has been published in Cell. The Moffat laboratory maintains a website...Carlson RCIscript-GoldyTALEN, pC-GoldyTALEN Tom Ellis pTAL5-BB, pTAL6-BB David Grunwald pCS2TAL3-DD,...Center has kindly constructed and deposited an unpublished set of Zika gene plasmids to Addgene’s repository... prototrophy, can also aid in designing self-establishing metabolically cooperating (SeMeCo) communities...

...what is important. Choose 4 panelists (maybe 5) and no more. Too many panelists will negatively affect timing...profiles of all panelists to the moderator. The moderator and organizers should develop a list of 4-6 pre-...put the panelists on the same level as the audience. In my experience the best set-up is panelists in a ... the panelists introduce themselves. I do a 1-2 minute introduction. If you chose good panelists, they...This post was originally published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates...They can result in lively discussions with both panelists and audience fully engaged. They can also be dull...subject area expert, but the moderator is not a panelist and will play a different role so her ability ...

...like ReFigure which aims to consolidate published and unpublished experiments on similar subjects in a single...are new publishing outlets like F1000, PeerJ, PLoS, and bioRxiv that make it easier to publish (or pre-publish...highly recommend listening to the panel discussion to learn a bit more about how the publishing community views...speakers from academia, industry, nonprofits, and publishing. The livestream video from the event can be found...recommend watching the whole livestream for a more holistic understand of reproducibility issues and their...are quite low (<30%, Prinz et al 2011, Begley and Ellis 2012). Recent efforts from the Reproducibility Project...the abbreviated methods section found within a published manuscript. Luckily, many organizations have been...

...day, I cross items off as they are accomplished or I add to the list as the need arises.Why do I write ...have a good mixture of useful tasks that can be accomplished quickly and things that will take longer. Completing...will swiftly give you the wonderful feeling of accomplishment while the longer tasks will give you larger...larger payoffs later. Don’t be afraid to accomplish some quick but moderately useful tasks at the expense ...the long and highly useful tasks. The quick accomplishments will likely energize you. For example, let'...it. The other half of each day you can spend accomplishing small tasks (like... preparing plasmids to send...experiment fails, you’ll still have made small accomplishments each day that should help keep you content ...

...table to search and filter the list of citing articles. From the article list you can click on the PubMed...Plasmids" lists Properly citing plasmids you've requested from Addgene may get your paper listed in the...This Plasmid" list increasing your work's visibility Help us improve our citations lists We are still ...in other publications, you'll find a link to the list of citing articles under the "Resource Information...Information" heading, you'll be directed to a page listing the articles that cite this plasmid. You can use...use the dropdown to increase the length of the list (purple oval in the screenshot below). You can also...you from performing any unnecessary cloning - the list of "Associated Plasmids" from these citing articles...

...onto data longer before publishing. But there are strong incentives to publish such as precedence, recognition...sharing policy. Since early March, the open access publisher has required authors to include a note as to where...result of consultations between researchers and publishers. Nonetheless, the initial release caused a storm...increasing the ability of reviewers (and, once published, anyone) to detect possible errors in assumptions...time to make sense of it, with their decision to publish setting the clock for others to dive in. The real... video, generating huge amounts of data. When published, this data is usually condensed into a statistically...clarified what the policy intent was and provided an FAQ list to further explain the policy. The fact that so ...

...plasmid(s) online (left), choose to submit published or unpublished plasmid(s). Fields marked with an asterisk... the online form you can submit "Published plasmids" or "Unpublished or pre-publication plasmids" and ...plasmids. You can submit "Unpublished or pre-publication plasmids": Unpublished plasmids or plasmids that... desired distribution status. You can submit "Published plasmids": This option allows you to quickly associate...appropriate publication. This publication will be listed at the top of the final plasmid pages and scientists...that will not be published are accepted. Addgene also encourages submitting pre-publication plasmids so...so they can be available when the paper is published. Plasmids can be linked to a peer-reviewed manuscript...

... the plasmid has been published, the paper in which it was first used is listed on the plasmid page.  ...sequences for use, these are listed.  We also provide a link to our list of common primers used for sequencing...construct was made. The depositor name links to a page listing all of all this PI’s plasmids that are available...  The paper link leads to a page listing all the plasmids from this paper that are available through Addgene...common vector backbones.  The backbone modifications listed can refer to changes made to the original backbone...vector previously deposited with Addgene, you can list the original vector’s plasmid number here. Gene/...gene/insert section on the plasmid page. Tags are listed according to whether they’ll end up on the N or...

...scientific publishing world and debate issues about how to improve the scientific publishing process. There...specialty, who may be other scientists, or science journalists, or policy makers. The term editor covers a wide...variety of careers and jobs. At Science, which is published by the American Association for the Advancement...sorts of different editors. Do you like science journalism? Take a look at our news editors. A fondness ...with things like word choice, punctuation, and stylistic consistency. If you’ve ever doubted the importance...as financially critical. Those who work on the publishing side of science have professional meetings, societies...Kitchen’ for a glimpse into the world of scholarly publishing. And see for example the Eighth International...

...topic or that were published by the lab down the hall.  Scientific journals: They publish papers, so naturally...of the number of papers with your search term published each year, which is a nice way to see growth or...receive a daily, weekly, or monthly digest of newly published papers that contain that term. You can also set...your field of research or larger journals that publish about a broad range of topics.  Twitter paper bots...keyword search that’s constantly updated. This Twitter list contains >300 Twitter literature bots, including...graph of the number of papers with your key term published each year is displayed with your search results...searches to particular authors, with the authors who published the most papers with your keyword populated at...

...by researchers for whom English is a second or third language (making publishing their research all the...research project, the vast majority of what gets published in scientific journals flies under the radar and...have time to keep up with all the research being published on our own topic. Only on occasion do we then ...   Video abstracts about research, especially published research, have grown in popularity over the last...this: “In terms of total view count and videos published, YouTube reported a total of 865,995 views for...online article downloads – especially if they are published in open access journals.   Check Out Addgene Videos...Facebook is continuing its growth, and while many publishers and researchers have Facebook pages, few share...

...thiamine metabolism Requires exogenous thiamine for growth ara disruption of arabinose metabolism pathway...NEB has a great list of genetic markers for your reference. Browse Addgene's curated list of Bacterial Expression...proteins needed for bacterial conjugation. Genes listed on F´ are wild-type unless indicated otherwise ... Phage resistance gal Mutation in galactose metabolism pathway Cells cannot grow on galactose only ...repressed more   LacZ β-galactosidase activity abolished   lacY Lactose permease inactivated, lactose...plasmid stability recBCD Exonuclease V activity abolished Increased plasmid stability, reduced recombination...r-m+)  MG1655    Wild type K-12 strain; first published sequence of an E. coli K-12 strain. F- λ- ilvG...

...recent newsletters. Listen to the entire Hot Plasmids episode! We generally publish 5-10 Hot Plasmid articles... Plasmids Segment. Click on the player below to listen to a quick (~5 min) Hot Plasmids podcast that introduces... for making diverse, localized point mutations. Listen to Our Segment on CRISPR X Hot Plasmid 2: Magneto...Control neuronal activity with a magnetic field. Listen to Our Segment on Magneto 2.0 Hot Plasmid 3: The...expression of His-tagged human kinases in bactera. Listen to Our Segment on The Human Kinase Domain Constructs... unused auxotrophies in Saccharomyces strains.  Listen to Our Segment on The Yeast Prototrophy Kit Special... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

...catalog either list a bioRxiv preprint as the source or were deposited when the now-published article was... with each other: from formal mechanisms like publishing a research article, presenting a conference poster... public server independent from peer-reviewed publishing in a journal.  Accelerating Scientific Communication...while peer review is valuable, modern scientific publishing has a number of drawbacks, especially the increasingly... admittedly, somewhat meaningless) practice of listing “Submitted” articles in their CV or grant applications...scientific communication needs. Going to present some unpublished results at a conference? Post your working manuscript...scientific community. Most preprints are eventually published in journals after formal peer review, with the...

...best way to share these improvements? You could publish a paper that happens to include your improvements...dissemination of your improved protocol. Many scientists, publishers, and small start-ups are turning to software ...example of a plasmid with a downloadable protocol listed under “Supplemental Document”: gRNA_Cloning Vector...Android). A neat feature of protocols.io is the checklist feature which allows you to check off steps in...Addgene we’ve been known to recommend protocols listed on OWW, including those for preparing your own ...online protocol repository hosted by the Nature Publishing Group. Like the previously mentioned sites, NPE...site allows you to comment on protocols and create lists of your favorite protocols for future reference....

...a resource checklist. A budget is an account of the material resources needed to accomplish your science...of creating a start up budget, create a start up list.  In this post we’ll help you successfully negotiate...discovery will need, and how long it will take to publish based on these findings. Most often we suggest ...then start identifying the resources needed to accomplish that goal. 2. Identify the tools in that timeline...timeline Avoid writing down prices as you create your list. This helps to ensure that all of the resources ...such as pipet tips, glassware, etc), get a simple list from your current P.I., lab manager, or administrator...have to be complicated, or difficult to create this list. Start BEFORE searching for a faculty position. ...

...of which can be listed here. If you don’t see the gene you’re looking for on this list, please consider...in a collection that grows on a daily basis, the lists below provide a snapshot of relevant resources and... and a good jumping off point. The following lists of PIs, articles, genes, and blog posts are not comprehensive...just because you do not see a name or gene on this list, that does not necessarily mean the repository does...PI pages on the Addgene website. In-exhaustive List of Addgene Depositors Who Study Chromatin Cheryl... available from Addgene The links below pull up lists of plasmids containing the indicated gene, and you... The links below direct you to pages containing lists of plasmids from the indicated publications. You...

...our newsletter or on our hot plasmids webpage. Listen to the entire Hot Plasmids podcast! Hot plasmids...transgenic lines to study neurodegenerative diseases. Listen to Our Segement on the Tol 2 Gateway-Compatible...AAV Vectors for tageting GABA-ergic interneurons. Listen to Our Segment on these new AAV Vectors Hot Plasmid...monomeric, and cyan-excitable red fluorescent protein. Listen to Our Segment on mCyRFP1 Hot Plasmid 4: mScarlet... bright, and monmeric red fluorescent protein.  Listen to Our Segment on mScarlet Special thanks to our... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer....     Additional Resources on the Addgene Blog Listen to Our Podcast Interview with Eye Researcher Connie...

...Multiple first or corresponding author publications (published, not in preparation) A history of successful funding...A project with sufficient preliminary data to establish your own lab and get an independent investigator...the beginning of Year 5. Now work backwards. To publish a submitted manuscript in a high-profile journal...typically have 4-5 years to make discoveries and publish papers to position yourself for a job search. Therefore...the best possible outcome, e.g. where could you publish this and how important is the result? Is there... much new data, speculate on what you hope to accomplish and how your research fits in with the current... meet future collaborators and get access to unpublished information and reagents. Part of the reason ...