...limited selection of fluorophores. If the available colors are already reserved for other markers in your ... all cells positively labeled with the selected color will not be CD8+ T cells and we can simply “dump...undesired cell populations. Dump gating uses the same color for different markers, making it easy to gate on...

...exchange vector. This vector contains sacB, an chloramphenicol resistance cassette, and the R6K origin of ...products. To allow manipulation of naturally chloramphenicol-resistant strains, we have also included versions...exchange system, he is so excited to use it to explore how hospital-associated Gram-negative bacteria ...

...targets with different colors. Multiplex immunofluorescence Speaking of different colors, having a palette ...secondary antibodies in a few different species and colors makes it easy to perform multiplexed IF. By using...

...with many challenges, researchers have begun to explore genome editing approaches for exon skipping. Addgene...diseases will require HDR-mediated precision editing tailored to smaller patient populations. To bring DMD CRISPR... Cas9s such as eSpCas9 and SpCas9-HF should be explored to reduce off-target editing. Short-term CRISPR...

...rural Africa Once this position ended, I wanted to explore another area which I was really passionate about...returning from Ghana at the beginning of 2010, I explored options for going into parasitology but another... for young scientists and PhD students to also explore a range of alternative careers outside of acidemia...

...is a PhD trained scientist at the University of Colorado, Anschutz Medical Campus.  He studies viruses ...currently a postdoctoral fellow at the University of Colorado Anschutz Medical Campus in Aurora, CO where she.... Erin is also the President of Project Bridge Colorado, an advocacy initiative that seeks to bridge the...

...cytometry experiment uses 2 or 3 different fluorescent colors each measuring a different target. Flow cytometers...versatile and some can accommodate up to 30 different colors. This lends flow cytometry particularly well to...causing dead cells to fluoresce.  When running a multicolor flow cytometry experiment, users must also prepare...

...an action potential. Halorhodopsins (HR) are green/yellow-light sensitive chloride ion pumps, activation...activation of which results in efflux of chloride ions, subsequent hyperpolarization, and inhibition of action...

...Cas9 in the apo state (PDB ID 4CMP) with domains colored as in Figure 1. HNH, red; RuvC, orange; CTD, yellow...mismatches between base pairs 18 and 20.   Pro tip! The Taylor lab mutated the residues in this loop to produce... L., Jung, K., McCool, R. S., Johnson, K. A., & Taylor, D. W. (2022). Structural basis for mismatch surveillance...

...United States including green cards that are well-tailored to meet the needs of researchers. The categories...and the arts, and requiring the attainment of a bachelor's degree or its equivalent as a minimum (8 U.S....). The foreign worker must possess at least a bachelor's degree or its equivalent and state licensure,...

....”- Joanne Kamens, Executive Director Career exploration “You can't wait for opportunities to fall in .... But there were a few teachers and 'guidance counselors' along the way who thought it was risky, "not...

...methods in this blog post. 8. Chromoproteins: Colorful Proteins For Molecular Biology Experiments by ...the fluorescent protein family and they generate color! They've been adapted for many molecular biology...

... who passed away in 2008. His work exposes and explores the hidden structures within flowers with the ...technique provides scientist with a new method to explore the inner structures of delicate plants and other...

...]. With the advent of CRISPR/Cas9, we began to explore the paired double-strand break (DSB) approach at...ectopic reporter assays. We have been interested in exploring the function of non-coding regulatory elements...

...fluorophore tagging vectors for your single or multi-color imaging experiments. Interested in epitope tags?... Julian Taylor-Parker...

...highlight our blog, daily updates, and offer better exploration throughout the site.  Blugene gets a make-over...AAV friend.  We encourage you to take a look and explore the new homepage. We hope every time you come to...

...responsibilities on the QC team, I was able to explore other parts of Addgene. This was done through the.... Specifically as a co-op, it is encouraged to explore different teams and work on projects there. At ...

...science. “It ended up we moved to Colorado for my husband’s postdoc. Colorado is growing in the biotech sphere...

...and drag and drop an icon onto the workspace. The color and size of icons are adjustable. New icons can ...scientific graphics. The tools settings let you tailor your search to particular types of images. For ...

...opportunities for promotion. It’s not uncommon for bachelor’s and master’s level scientists to have job titles...Now? Academic v. Non-Academic Career Decisions Explore alternative science career options Resources on...

...more details on how to make the most of these exploratory conversations. If you set yourself a goal of ...to Find Funding For Your Research University of Florida: Training Grants and Other Funding Opportunities...

...Lab at Stanford University became interested in exploring biological dark matter – large tracts of the microbial... microbial tree of life that remained unexplored. Using new single-cell sequencing approaches, the lab...

... plants (from callus) or T1 plants (Arabidopsis floral dip). In the next generation the CRISPR construct...right) A RUBY-expressing Arabidopsis seed following floral dip transformation next to an untransformed seed...

...school could afford – led me to develop BioBits ExplorerTM and BioBits BrightTM, biology kits based on freeze-dried...   References 1. Huang, Ally, et al. "BioBits™ Explorer: A modular synthetic biology education kit." Science...

...entities with a large range of characteristics (color, brightness, photostability, maturation, oligomerization...Protein Choosing Your Fluorescent Proteins for Multicolor Imaging Resources on Addgene.org Browse all...

...companies. The space was modern with clean bold colors of orange and white. The decor was very contemporary...Addgene lab, we don’t often get the opportunity to explore new techniques in our own space. Workshops such...

...thawed the samples at room temperature and used the color of the liquid solution and pH paper to measure the...samples were at the control pH of 7.4 as indicated by color (data not shown). What we did next: Venting the ...

...actually want, you can feel lost. Use this post to explore the wide range of careers available to scientists...roles. These types of skills show that you fully explore the different options available and then decide...

...plant compounds to treat complications of diabetes Gloria Otito Izu, PhD Candidate, University of Ibadan,...Oluwatosin Adeyi, University of Ibadan, Nigeria Gloria Otito Izu is a PhD Candidate working under the ...

... in the presentation, starvation or the use of caloric restriction mimetics trigger autophagy and, in ... References: 1. Pietrocola, Federico, et al. "Caloric restriction mimetics enhance anticancer immunosurveillance...

...in the lab. This is commonly done using calcium chloride which permeabilizes the cell membrane so the bacteria...phases of competence occur during the Helicobacter pylori growth cycle." FEMS microbiology letters 255.1 ...

... ChIP is an incredibly useful application for exploring these interactions, and can give valuable insight... with a PCR purification kit or with a phenol-chloroform extraction.    Fig. 1: Schematic overview...

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...Chicken IgY domains are especially useful for multi-color staining of tissue because they have limited cross-reactivity...cultured cells and tissue sections Great for multicolor staining of tissue since it won’t cross-react...

...Hyperpolarizing chloride channel SwiChr++ - A channelrhodopsin converted into a chloride-conducting anion...

...tried-and-true protein expression control tools as well as explore some new innovations. Whether you want to turn ...introduced gene or over the native locus? Let’s explore each of the options together. Turn it off Turn...

...tdTomato and assessed cFos expression. She will explore the connectivity patterns of these cells and the...during morphine administration and withdrawal to explore how these cells may encode the negative affect ...

...reporter gene has over the years evolved from chloramphenicol acetyltransferase to luciferase, the basic ... Professor of Molecular and Cellular Biology at Baylor College of Medicine and Editor-In-Chief of the ...

...standard method for purification of rAdV uses cesium chloride (CsCl) density gradients combined with ultracentrifugation...practical guide for using the AdEasy System. 3. Jager, Lorenz, et al. "A rapid protocol for construction and ...

...synapses or that are Cre-inducible, researchers can explore synaptic integration and connectivity without the...Guo and her colleagues at Yale University fused a color-changing fluorescent timer (FT) protein to the histone...