We narrowed to 619 results for: LIS

...PDB ID 7S4X). The end of the DNA:RNA heteroduplex lies between the RuvC (orange) and HNH (red) domains,... will destabilize the nascent heteroduplex and abolish cleavage (Pattanayak et al., 2013). As mentioned...

...Wilson, Ning Sun, Deepak M. Gupta, Mei Huang, Zongjin Li, Nicholas J. Panetta, Zhi Ying Chen, Robert C. Robbins...reported in González et al. A percentage is not listed because many variables (cell type, criteria used...Check out the links at the end of the post for a list of Tet-inducible reprogramming vectors available...

... linker (see Figure 1). The key to the biosensor lies in pH: DsRed is relatively pH-insensitive, while...plasmids in a future blog post. No time to read? Listen to our hot plasmids segment on the Addgene Podcast...constructed. The authors then used GMAP assembly to establish a collection of over 30 promoters and 140 genes...characterize positive hits. This work has been published in Cell. The Moffat laboratory maintains a website...Carlson RCIscript-GoldyTALEN, pC-GoldyTALEN Tom Ellis pTAL5-BB, pTAL6-BB David Grunwald pCS2TAL3-DD,...Center has kindly constructed and deposited an unpublished set of Zika gene plasmids to Addgene’s repository... prototrophy, can also aid in designing self-establishing metabolically cooperating (SeMeCo) communities...

...what is important. Choose 4 panelists (maybe 5) and no more. Too many panelists will negatively affect timing...profiles of all panelists to the moderator. The moderator and organizers should develop a list of 4-6 pre-...put the panelists on the same level as the audience. In my experience the best set-up is panelists in a ... the panelists introduce themselves. I do a 1-2 minute introduction. If you chose good panelists, they...This post was originally published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates...They can result in lively discussions with both panelists and audience fully engaged. They can also be dull...subject area expert, but the moderator is not a panelist and will play a different role so her ability ...

...like ReFigure which aims to consolidate published and unpublished experiments on similar subjects in a single...are new publishing outlets like F1000, PeerJ, PLoS, and bioRxiv that make it easier to publish (or pre-publish...highly recommend listening to the panel discussion to learn a bit more about how the publishing community views...speakers from academia, industry, nonprofits, and publishing. The livestream video from the event can be found...recommend watching the whole livestream for a more holistic understand of reproducibility issues and their...are quite low (<30%, Prinz et al 2011, Begley and Ellis 2012). Recent efforts from the Reproducibility Project...the abbreviated methods section found within a published manuscript. Luckily, many organizations have been...

...day, I cross items off as they are accomplished or I add to the list as the need arises.Why do I write ...have a good mixture of useful tasks that can be accomplished quickly and things that will take longer. Completing...will swiftly give you the wonderful feeling of accomplishment while the longer tasks will give you larger...larger payoffs later. Don’t be afraid to accomplish some quick but moderately useful tasks at the expense ...the long and highly useful tasks. The quick accomplishments will likely energize you. For example, let'...it. The other half of each day you can spend accomplishing small tasks (like... preparing plasmids to send...experiment fails, you’ll still have made small accomplishments each day that should help keep you content ...

...table to search and filter the list of citing articles. From the article list you can click on the PubMed...Plasmids" lists Properly citing plasmids you've requested from Addgene may get your paper listed in the...This Plasmid" list increasing your work's visibility Help us improve our citations lists We are still ...in other publications, you'll find a link to the list of citing articles under the "Resource Information...Information" heading, you'll be directed to a page listing the articles that cite this plasmid. You can use...use the dropdown to increase the length of the list (purple oval in the screenshot below). You can also...you from performing any unnecessary cloning - the list of "Associated Plasmids" from these citing articles...

...onto data longer before publishing. But there are strong incentives to publish such as precedence, recognition...sharing policy. Since early March, the open access publisher has required authors to include a note as to where...result of consultations between researchers and publishers. Nonetheless, the initial release caused a storm...increasing the ability of reviewers (and, once published, anyone) to detect possible errors in assumptions...time to make sense of it, with their decision to publish setting the clock for others to dive in. The real... video, generating huge amounts of data. When published, this data is usually condensed into a statistically...clarified what the policy intent was and provided an FAQ list to further explain the policy. The fact that so ...

...(s) online (left), choose to submit as published or unpublished. Fields marked with an asterisk are required... the online form you can submit "Published plasmids" or "Unpublished or pre-publication plasmids" and ...plasmids. You can submit "Unpublished or pre-publication plasmids": Unpublished plasmids or plasmids that... desired distribution status. You can submit "Published plasmids": This option allows you to quickly associate...appropriate publication. This publication will be listed at the top of the final plasmid pages and scientists...that will not be published are accepted. Addgene also encourages submitting pre-publication plasmids so...so they can be available when the paper is published. Plasmids can be linked to a peer-reviewed manuscript...

... the plasmid has been published, the paper in which it was first used is listed on the plasmid page.  ...sequences for use, these are listed.  We also provide a link to our list of common primers used for sequencing...construct was made. The depositor name links to a page listing all of all this PI’s plasmids that are available...  The paper link leads to a page listing all the plasmids from this paper that are available through Addgene...common vector backbones.  The backbone modifications listed can refer to changes made to the original backbone...vector previously deposited with Addgene, you can list the original vector’s plasmid number here. Gene/...gene/insert section on the plasmid page. Tags are listed according to whether they’ll end up on the N or...

...scientific publishing world and debate issues about how to improve the scientific publishing process. There...specialty, who may be other scientists, or science journalists, or policy makers. The term editor covers a wide...variety of careers and jobs. At Science, which is published by the American Association for the Advancement...sorts of different editors. Do you like science journalism? Take a look at our news editors. A fondness ...with things like word choice, punctuation, and stylistic consistency. If you’ve ever doubted the importance...as financially critical. Those who work on the publishing side of science have professional meetings, societies...Kitchen’ for a glimpse into the world of scholarly publishing. And see for example the Eighth International...

...topic or that were published by the lab down the hall.  Scientific journals: They publish papers, so naturally...of the number of papers with your search term published each year, which is a nice way to see growth or...receive a daily, weekly, or monthly digest of newly published papers that contain that term. You can also set...your field of research or larger journals that publish about a broad range of topics.  Twitter paper bots...keyword search that’s constantly updated. This Twitter list contains >300 Twitter literature bots, including...graph of the number of papers with your key term published each year is displayed with your search results...searches to particular authors, with the authors who published the most papers with your keyword populated at...

...by researchers for whom English is a second or third language (making publishing their research all the...research project, the vast majority of what gets published in scientific journals flies under the radar and...have time to keep up with all the research being published on our own topic. Only on occasion do we then ...   Video abstracts about research, especially published research, have grown in popularity over the last...this: “In terms of total view count and videos published, YouTube reported a total of 865,995 views for...online article downloads – especially if they are published in open access journals.   Check Out Addgene Videos...Facebook is continuing its growth, and while many publishers and researchers have Facebook pages, few share...

...thiamine metabolism Requires exogenous thiamine for growth ara disruption of arabinose metabolism pathway...NEB has a great list of genetic markers for your reference. Browse Addgene's curated list of Bacterial Expression...proteins needed for bacterial conjugation. Genes listed on F´ are wild-type unless indicated otherwise ... Phage resistance gal Mutation in galactose metabolism pathway Cells cannot grow on galactose only ...repressed more   LacZ β-galactosidase activity abolished   lacY Lactose permease inactivated, lactose...plasmid stability recBCD Exonuclease V activity abolished Increased plasmid stability, reduced recombination...r-m+)  MG1655    Wild type K-12 strain; first published sequence of an E. coli K-12 strain. F- λ- ilvG...

...recent newsletters. Listen to the entire Hot Plasmids episode! We generally publish 5-10 Hot Plasmid articles... Plasmids Segment. Click on the player below to listen to a quick (~5 min) Hot Plasmids podcast that introduces... for making diverse, localized point mutations. Listen to Our Segment on CRISPR X Hot Plasmid 2: Magneto...Control neuronal activity with a magnetic field. Listen to Our Segment on Magneto 2.0 Hot Plasmid 3: The...expression of His-tagged human kinases in bactera. Listen to Our Segment on The Human Kinase Domain Constructs... unused auxotrophies in Saccharomyces strains.  Listen to Our Segment on The Yeast Prototrophy Kit Special... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

...catalog either list a bioRxiv preprint as the source or were deposited when the now-published article was... with each other: from formal mechanisms like publishing a research article, presenting a conference poster... public server independent from peer-reviewed publishing in a journal.  Accelerating scientific communication...while peer review is valuable, modern scientific publishing has a number of drawbacks, especially the increasingly... admittedly, somewhat meaningless) practice of listing “Submitted” articles in their CV or grant applications...scientific communication needs. Going to present some unpublished results at a conference? Post your working manuscript...scientific community. Most preprints are eventually published in journals after formal peer review, with the...

...Addgene might not be as high on the published-paper-celebration checklist as champagne or cupcakes, or maybe...We all know the timeline for publishing research papers can be long and unpredictable. Fortunately, you... hold from public access until the article is published. This policy gives us time to complete necessary...ready for distribution as soon as the paper is published, while giving you peace of mind that your materials...become aware the related journal article has been published we’ll get the plasmids online for distribution...for you to notify us as soon as your article is published. As much as we’d like to, we can’t keep up with...the plasmids available as soon as your paper is published helps maximize the potential impact of your work...

...a resource checklist. A budget is an account of the material resources needed to accomplish your science...of creating a start up budget, create a start up list.  In this post we’ll help you successfully negotiate...discovery will need, and how long it will take to publish based on these findings. Most often we suggest ...then start identifying the resources needed to accomplish that goal. 2. Identify the tools in that timeline...timeline Avoid writing down prices as you create your list. This helps to ensure that all of the resources ...such as pipet tips, glassware, etc), get a simple list from your current P.I., lab manager, or administrator...have to be complicated, or difficult to create this list. Start BEFORE searching for a faculty position. ...

...of which can be listed here. If you don’t see the gene you’re looking for on this list, please consider...in a collection that grows on a daily basis, the lists below provide a snapshot of relevant resources and... and a good jumping off point. The following lists of PIs, articles, genes, and blog posts are not comprehensive...just because you do not see a name or gene on this list, that does not necessarily mean the repository does...PI pages on the Addgene website. In-exhaustive List of Addgene Depositors Who Study Chromatin Cheryl... available from Addgene The links below pull up lists of plasmids containing the indicated gene, and you... The links below direct you to pages containing lists of plasmids from the indicated publications. You...

...our newsletter or on our hot plasmids webpage. Listen to the entire Hot Plasmids podcast! Hot plasmids...transgenic lines to study neurodegenerative diseases. Listen to Our Segement on the Tol 2 Gateway-Compatible...AAV Vectors for tageting GABA-ergic interneurons. Listen to Our Segment on these new AAV Vectors Hot Plasmid...monomeric, and cyan-excitable red fluorescent protein. Listen to Our Segment on mCyRFP1 Hot Plasmid 4: mScarlet... bright, and monmeric red fluorescent protein.  Listen to Our Segment on mScarlet Special thanks to our... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer....     Additional Resources on the Addgene Blog Listen to Our Podcast Interview with Eye Researcher Connie...

...Multiple first or corresponding author publications (published, not in preparation) A history of successful funding...A project with sufficient preliminary data to establish your own lab and get an independent investigator...the beginning of Year 5. Now work backwards. To publish a submitted manuscript in a high-profile journal...typically have 4-5 years to make discoveries and publish papers to position yourself for a job search. Therefore...the best possible outcome, e.g. where could you publish this and how important is the result? Is there... much new data, speculate on what you hope to accomplish and how your research fits in with the current... meet future collaborators and get access to unpublished information and reagents. Part of the reason ...

...communication activities, e.g. tweet it when you publish a new blog post, and live tweet from conferences... If you live in the UK, join the SciComm mailing list. 7) Internships – Learn on the job Nothing prepares...internship at a magazine or newspaper (here’s a partial list). A great opportunity is the AAAS Mass Media Fellowship...into science communication by taking a media or journalism course. Courses can help you gain work experience...like Northwestern University or UC Berkeley have journalism graduate schools where you may take a science...research and residential communication. If science journalism is the path you want to follow, check out the... at the National Council for the Training of Journalists (NCTJ) or the Alan Alda Center for Communicating...

...introduced into the B. subtilis genome. Mobile-CRISPRi is then transferred from B. subtilis to the bacterium...colonies, but some bacteria, including E. faecalis and P. mirabilis, had only ~1 transfer per 1,000,000 colonies...CRISPRi into a single bacterial strain, such as a B. subtilis, or a narrow range of bacterial species, such ...integrative and conjugative elements in Bacillus subtilis (ICEBs1) system. ICEBs1 carries the genes needed...bi-parental mating. Following excision from the B. subtilis genome, the Mobile-CRISPRi ICE element forms a...integrative and conjugative element in Bacillus subtilis." Plasmid 86 (2016): 14-25. PubMed PMID: 27381852...

...best way to share these improvements? You could publish a paper that happens to include your improvements...dissemination of your improved protocol. Many scientists, publishers, and small start-ups are turning to software ...example of a plasmid with a downloadable protocol listed under “Supplemental Document”: gRNA_Cloning Vector...Android). A neat feature of protocols.io is the checklist feature which allows you to check off steps in...online protocol repository hosted by the Nature Publishing Group. Like the previously mentioned sites, NPE...site allows you to comment on protocols and create lists of your favorite protocols for future reference....inventory at your finger tips, and make shopping lists as you finish the last of a reagent. Though sharing...

...the information. In contrast, a list of experiments or a laundry list of molecules to screen has unclear...Either way, think realistically about the time and resources required to feasibly accomplish the aims and ...proteins regulate the Z pathway?" is an open-ended list of proteins. Instead, consider "how do proteins ...exist to solve the problem. What will you have accomplished in 5-10 years? You need to be able to describe...learned and/or now be possible to do? Research accomplishments are remembered for the problem solved, not ...chalk talk with questions from the committee. For a list of common questions, check out this eBook about ...

...complicated! Listen to the full podcast for more great science communication tips or listen to the chapters...topics discussed during the interview. Happy listening! Listen to the interview with Science in the News ...Pint series. Chapter 4: The SITN Podcast, Sit'N Listen - In their podcast, the SITN team brings in scientists...like to develop the SITN podcast as a means for listeners to understand the dynamics of information flow... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

...stupendous listener, but I aspire to  be. If you don’t at least aspire to be a good listener and consciously...brought to the table was empathy, a willingness to listen, and an ability to remember the right stories at...disagreeable, you recognize their feelings as valid. Listening: In one of my favorite books, Michael Ende’s Momo... nearby village (and to the reader) by simply listening. Chances are, you know someone like this, even...Ende, courtesy of morefamousquotes.com A good listener absorbs and remembers and makes the teller feel...collaboration may not be the easiest resolution to accomplish, but there is a potential for long-term gains...examples of times when you have used your empathy, listening skills, and institutional knowledge to resolve...

...annurev-neuro-111020-100834 Challis, R.C., Ravindra Kumar, S., Chan, K.Y., Challis, C., Beadle, K., Jang, ...or lumbar spine. Image and caption courtesy of Challis et al., 2022. Where do they come from? Systemic...Addgene’s Data Hub and the CLOVER repository of published systemic AAVs to find commonly used vector cargo...capsid, while Table 1 gives a brief overview of published descriptions of commonly used or emerging capsids...https://clover.caltech.edu. Figure adapted from Challis et al. 2022. Image created with BioRender.com. ... plasmids available from Addgene Capsid Published Tropism Model Organism Mouse Strain-Specific...Strain-Specific Tropism?" column. For a complete list of mice strains with LY6A-dependent tropism, see...

...citations in its first four years (1). In addition to publishing in Science, Professor Zhang deposited the associated...CRISPR/Cas papers and Addgene deposits, but papers published in other years also get many more citations This...For example, it is very plausible that science published in more widely-read journals gets more citations...adjust our estimates based on the journal that published the articles.  That is, we re-estimate the citations...associated with Addgene deposits as if they were published in the same journals as those that did make deposits...papers with Addgene plasmids in our data were published in Nature, but only 3 percent of papers without... Weighting Example Article Number (A) Year Published (B) Number of Citations (C) Journal Name (D) Percent...

...and why other publishers and organizations have various different versions of checklists and guidelines...communication falls on the shoulders of journals and publishers. As journal editors, we often find ourselves ...contributions, etc.) before their paper can be published. To a scientist, all of this checking and formatting...communication further up the collective priority list. There has been progress—for example, a number of... of other Cell Press journals, including Cell Metabolism, Cell Reports and Joule. In September 2017 she...reproducible Read all of our blog posts on scientific publishing  ...

...for controlling molecular processes with light. Listen to Our Segement on PhoCl Segment  Hot Plasmid 2...selection for increased CRISPR editing efficiency. Listen to the Segement on Ouabain Selection  Hot Plasmid...base editors with enhanced range and specificity. Listen to to the Segmennt of Improved CRISPR Base Editors...Plasmids for generating DNA molecular weight markers.  Listen to the Segement on the pPSU Plasmids Special thanks... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer....     Additional Resources on the Addgene Blog Listen to Our Podcast Interview with Eye Researcher Connie...

...Pasteurella multocida, Porphyromonas gingivalis, Proteus mirabilis, Providencia spp., Pseudomonas spp.,...stability, which influence the plasmid’s ability to establish and maintain itself within host cells.  The plasmid...bacteria in lab manipulations. Table 1 has a detailed list of the bacterial species these plasmids are compatible...compatible with.  Table 1: Non-exhaustive list of common broad host range plasmids and the bacterial species...Myxococcus virescens, M. xanthus, Proteus rettgeri, P. mirabilis, Providencia stuartii, Pseudomonas spp., Rhizobium...campestris, X. campestris pv. malvacearum, Zymomonas mobilis pBBR1 based pBBR1  Gram-negative  Alcaligenes...

...cells). pBACKBONE-XXXXX Tip: For a catalog of published and commercially available empty backbones, visit...fusions that are on your insert. Typically you would list any tag or fusion protein in the order they appear... tag on the N-terminal of your insert, you would list it first. pBACKBONE-Flag-hGene If there was also...fused to the C-terminal of your insert you would list it after the insert. pBACKBONE-Flag-hGene-EGFP Other...included in the plasmid name. Mutations are generally listed as the amino acid change and not a nucleotide change...proper way to denote an amino acid mutation is to list the one letter abbreviation of the wild type amino...

...2013 there have been 33 CRISPR software tools published and documented by OMICtools. It must be confusing...The first of only three such databases, EENdb (published 1 Jan 2013, no longer available), is a simple ...sgRNA designs. The second database, CrisprGE (published 27 Jun 2015), is noteworthy for its broad scope... content, and ease of access. The third, WGE (published 15 Sep 2015), is more similar to EENdb than CrisprGE...in detail, but that is already covered in the published articles. Instead, I have broken down the tools...or another. Supported organisms: This section lists the genomes supported by each tool. The genomes ...agnostic and have currently available organisms listed in this section. CRISPR software advice for specific...

...conversation. Goals and Planning Worksheet: Lists accomplishments, research/professional goals and feedback...credit for what we have accomplished. Starting with a recap of accomplishments, from learning new techniques...time has gone. Are we any closer to graduating, publishing that key paper, or figuring out a career path...tangible research and career goals, and celebrate accomplishments. These techniques are laid out in Vincent et...

...Organizing your lab notebooks, protocols, and to do lists can be easier than you think! Here are three tips...the colors and text. Tip 2: Use electronic to-do lists to access your tasks on the go Never miss a deadline...everything to your calendar. Ditch the paper To Do List and move to one that moves with you. Use software...software such as Evernote or Google Tasks to create lists of your upcoming tasks, set deadlines, and arrange...attachments. Organize your notes into notebooks, create checklists that link to other notes, and use the search ...spreadsheets for reagent storage locations, such as the list used to organize your cryo freezer. No more issues...

...Jessica Polka from Harvard Medical School and a long list of supporters from all of the research centers in...problems resulting from past practices and mores.  His list is dire:  The current system is unsustainable, hypercompetition...be radically overhauled.  Finally, the science publishing system needs to be changed.  Dr. Bourne charged...training excellence to begin effecting change.  Panelists call for action Each of the speakers in the panel...applause when he talked about how he boycotts publishing in all but open access journals.  Dr. David Glass...astounding lack of reproducibility being recognized in published work even in the “best” journals.  He pushed for...

...we'd like to extend a special thank you to Dr. Tom Ellis (Imperial College London), Dr. Connie Cepko (Harvard...asked leading professors for their top 3 tips. Tom Ellis 1. Keep up with the literature. He recommends setting...too; both with your research and with getting published.” Connie Cepko 1. Work hard. There will be periods...mentees who have goals outside of the university? Ellis recommends that a student interested in leaving ...engineering (STEM) expertise in law, business, journalism, entertainment, politics, K-12, etc.” Addgene's...Acknowledgments Thank you again to Connie Cepko, Tom Ellis, and George Church for answering our burning questions...

...for Microbiology (ASM) Scientific Writing and Publishing Institute. Writing is the cornerstone of any ... them with the world. If one doesn’t write and publish in some way, then the data produced isn’t likely...manuscripts and the ASM Scientific Writing and Publishing Institute (SWPI) is an extremely beneficial guide...should possibly take a composition class in the English Department. He suggested that I sign up for the...the SWPI online course because a course in the English department wouldn’t have trained me in the scientific...Resources on the Addgene Blog Dos & Don'ts When Publishing a Scientific Manuscript Writing Scientific Manuscripts...

... a neuroscience major, I was struck with the realisation that I didn’t want a career in neuroscience—whatever... to once again write for fun eventually saw an English minor tacked onto my BSc. With this decision I ...towards a career in science communication, an established field of inquiry with which I was not yet familiar...meaningful day job only came into focus when an English tutor noted my eclectic mix of science and arts... a scientific background and bring with them established interests and areas of expertise. Perhaps the... research explores risk-taking behavior among collision sport athletes, as well as the ethical and health...

...magazine. Humans are built to enjoy, remember, and listen to narratives — take advantage of that in your ...stories — whether I’m working with a talented journalist to shine light on living as a scientist in Beijing...research struggles in papers. They recount accomplishments in talks and interviews. They even tell short...tell good stories, they engage their readers or listeners thus expanding the impact and appreciation for...Interviewee: “I did the most work for a paper we were publishing, but when it came time to add author details ...which had affected his judgement. Eventually, we published the paper in a high-impact journal and it worked...

...she would be looking at where I had published, who I had published with and whether I would be able to... set up an ELISA kit company on whose website you can find some great information on ELISA troubleshooting...Undergraduate sections and brought in more focus on the list of conferences I presented at and placed them under...troubleshooting, ELISA kits and cell death assays. You can find Seán on twitter @Macfhearraigh.    Read more posts...

...and foster an effective working environment? Tom Ellis was offered a start-up package that included a postdoc... most surprising thing about becoming a PI? Tom Ellis has a one word answer: “Email. Nothing prepared ...differently or the same as your adviser did? Tom Ellis merges the influence from his two PhD advisers who...who were “chalk-and-cheese”. While one was an established Professor who was always “off flying his plane...our latest results as soon as the gels ran.” Dr. Ellis tries to lead his team as a scientists and a friend... lab!”   Acknowledgments Thank you to Dr. Tom Ellis (Imperial College London), Dr. Connie Cepko (Harvard...

...imaging Improving Well-established High-throughput assays Available Well-established Natural and engineered...Commons. The North American firefly, Photinus pyralis, catalyzes a chemical reaction in its abdomen to...systems. Our luciferase collection page has a detailed list of luciferase options. Table 2: Properties of luciferase...Cofactors Secreted North American firefly (Photinus pyralis) Natural or codon-optimized 562 61 Luciferin ATP...Furimazine None Optional AkaLuc(derived from Photinus pyralis) Engineered   677 61 AkaLumine ATP No Luciferase...

... their precise positions within the plasmid. The list of enzymes that can be detected is 5x greater than...GenBank File or a SnapGene file for the sequences listed. You can then analyze the sequence in your sequence...Sequence” tabs. Features The table found here lists the common features detected in the plasmid sequence... map, direction (if relevant), and types. Types listed are standard GenBank feature types. Clicking on...Sequence” tabs. Primers This tab contains a table listing commonly used primers detected in a given nucleotide...Analyzer guide A quick guide to all of the tabs listed above and their functions. We hope that these...

...pre-clinical life science work, economics) that published research is difficult to replicate.  Why is replicating...appreciate how few details are provided in most published papers. The precise timing, order, or every combination... rarely exist or are preserved as part of the publishing process and become lost if not archived. Remove... article is given “in-principle acceptance” to publish the final results regardless of the outcome of ...null findings that are notoriously difficult to publish.  With Registered Reports, the authors are able...scientific research. These include policies for publishers and funders in the Transparency and Openness ...

...recent plasmid deposit please sign up here.      Listen to this episode of the Hot Plasmids podcast Light-mediated...transmission Article contributed by Klaus Wanisch  Listen to the optogenetics podcast segment An optogenetic...and joined each fragment with one part of an established pair of proteins (iLID and SspB) that undergo...mammalian hosts Article contributed by Lukas Morgan  Listen to the acoustic reporter gene podcast segment In...regulation Article contributed by Michael Lemieux  Listen to the miRNA reporter podcast segment Discovered... CHIME Article contributed by Aliyah Weinstein  Listen to the CRISPR podcast segment Understanding the...

... plasmid deposit please sign up here.          Listen to this episode of Hot Plasmids! Optogenetic control...by CASANOVA Article contributed by Cary Valley  Listen to the anti-CRISPR podcast segment Anti-CRISPR ...using blue light (Bubeck et al., 2018). They accomplished this by inserting a photo-sensitive LOV2 domain... Archon Article contributed by Shreya Vedantam  Listen to the voltage indicator podcast segment The neurons... system Article contributed by Michelle Cronin  Listen to the 2in1 cloning podcast segment Recently, the...and AsLOV2 Article contributed by Angela Abitua  Listen to the photoswitchable protein podcast segment ...

... Map & Network (CeNGEN) The CeNGEN goal is to establish a comprehensive gene expression atlas of every...publication for the worm community where one can publish brief novel findings. These results are normally...results. This a great resource for scientists to publish findings that don't fit into larger publications... that can be referenced. In addition the data published in microPublications is curated and added to other...but there are Drosophila and zebrafish articles published there as well.   Worm Development Dynamics Database...used for introducing point mutations that was published in a microPublication (Schwartz and Jorgensen,...

... impossible to interpret. Polishing the fiber optics with diamond polishing paper of sequentially increasing... second key point; the fiber optics need to be polished to significant smoothness to prevent drastic loss...its output with a light meter before and after polishing. Efficiency of the implant should be at least ... output. The same considerations apply to the polishing and preparation of the patch cable (although you...it’s best to try those that have already been published first. Because behavioral optogenetics is performed...