We narrowed to 488 results for: mme

...the virus immediately upon harvesting. Interestingly, freeze-thawing host cells immediately prior to their...of cell culture medium on the packaging cells immediately following transfection. In other words, when ...

...results to a standard curve. For CNV assays, it is recommended to use a reference sample with two copies of ...Taqman chemistry (see image, left, of droplet immersion technique, courtesy of CAR15.) Each droplet contains... 29717442. Arya M, Shergill IS, Williamson M, Gommersall L, Arya N, Patel HR. Basic principles of real-time...

...Cell lysates are then treated with benzonase immediately before iodixanol purification. This approach ...production tips or tricks? Please share them in the comments below! Special thanks for Dr. Dan Stone and...AAV production and purification! References 1. Aurnhammer, C., Haase, M., Muether, N., Hausl, M.A., Rauschhuber...

...trained our models in close collaboration with the Krauthammer lab (University of Zurich), especially with my...F., Schmidheini, L., Solari, C., Balázs, Z., Krauthammer, M., & Schwank, G. (2023). Predicting prime editing...Ioannidi, E. I., Böck, D., Steensel, B. van, Krauthammer, M., & Schwank, G. (2024). Machine learning prediction...

...overexpression library) or is prone to recombination, we recommend amplification on solid media. If your plasmid ...recombination. If you do see recombination, we recommend ensuring the cells used are meant for repetitive...when developing your amplification protocol! We recommend looking at protocols used for libraries that are...

...beta-lactamase prior to division, instead of immediately experiencing the effects of the antibiotic. This...little more time, since bacteria won’t divide immediately after it has been heat-shocked or electrically...Resources References Hwang, J. M., Piccinini, T. E., Lammel, C. J., Hadley, W. K., & Brooks, G. F. (1986). ...

...but they cannot be entirely eliminated. It is recommended that you image samples before and after expansion...representation of reality. Third, expansion results in dimmer samples. Reduced signal can be caused by loss of...https://doi.org/10.1038/nbt.3625  Truckenbrodt S, Sommer C, Rizzoli SO, Danzl JG (2019) A practical guide...

...purification kits, it is low cost and can be immediately adopted by any laboratory. All procedures are...new DNA fragment, encode homology arms to allow immediate co-transformation with linearized vector backbone...This will minimize random errors during PCR: we recommend Phusion or Q5. Speed up your life Rather than ...

...reliable expression, and easy distribution as DNA (Trimmer, 2020), you may be interested in using them in ... effective compared to those aliquots frozen immediately post-harvest. Stress-tested antibodies that are...Purification Protocol Addgene's Antibody Guide References Trimmer JS. Recombinant Antibodies in Basic Neuroscience...

...company. The Addgene summer picnic This past weekend was the annual Addgene Summer Picnic - a time when...sometimes teams disagree. Yes, sometimes work is a bummer when you’d rather be at the beach or binging a ...

...conjugated to agarose or magnetic beads with commercially available chemical agents. Directly conjugating...Protein A/G to immobilize the antibody, it is recommended to preclear the sample to remove any proteins...low pH buffer such as 0.1M glycine pH 2.5 and immediately neutralized with Tris pH 8-8.5. Low pH glycine...

...the difference between materials available from commercial repositories and the same cell line from a colleague...difference is the QC and validation carried out by the commercial repository. Repositories generally ensure cell...mutation rate of the cell line, but it is broadly recommended that the lower the passage number the better ...

... the two fluorophore colours. Experts in FRET recommend to using two fluorophores that have a similar ...one that is better than another. It is often recommended by FRET experts to use as many measurement methods... Trends Biochem Sci. 2007 Sep; 32(9): 407-14. Zimmermann T,  Terjung S. FRET Basics and Applications: ...

...IPI’s anti-integrin rAbs are unconjugated and recommended for use in flow cytometry. Based on the established...resulting in cells that elongated and morphed asymmetrically (Jo et al., 2022). By contrast, LVDP-binding...conferred less force, leading cells to spread into symmetrical circles. While elucidating the role of tension...

...with almost 80% rating it as very important. One comment reflected the desire for a flexible generic solution...Important collaborative functionalities include comments, notifications, sharing files, assigning team ...actions. These records can take the form of written comments, added files, notes etc. Detailed records about...

...corresponding fluorophore-conjugated antibody commercially available. In other cases, fluorophore-conjugated...this system is that streptavidin conjugates are commercially available for a wide range of fluorophores, ...on the location of the targeted markers. Some commercially available fix/perm kits allow for labeling of...

...crossed strand. While this pathway may not be immediately applicable in DSB-induced genome engineering,... the vector. Alternatively, PCR-generated or commercially synthesized linear dsDNA templates can be used...catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA. Richardson CD, et al. Nat Biotech....

...DNA fragments. As a fun way to start, I highly recommend watching this entertaining video created by our...at 50 °C. After an hour or so, the sample is immediately ready to transform into competent cells. The ...contain the appropriate homologous sequences. NEB recommends an overlap of 15-40 bp, with a primer melting...

...addition of translation-blocking STOP cassette immediately following the promoter. The STOP cassette includes...homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells. Snippert HJ, van der...Freundlich T, Weissman TA, Schoppik D, Wang XC, Zimmerman S, Ciruna B, Sanes JR, Luchtman JW, Schier AF....

...sections you’re particularly interested in, but I’d recommend watching the whole livestream for a more holistic...pre-publish) sound data even if it isn’t considered immediately impactful. Down the line we may see further adoption...issues in the biological sciences. I’d highly recommend listening to the panel discussion to learn a bit...

...template and CRISPR machinery, Quadros et al. recommend ribonucleoprotein (RNP) complexes of Cas9 protein...splice donor/acceptor sites, Miura et al. (2018) recommend targeting sites at least 100 bp away from the ...infundibulum. In vivo electroporation is performed immediately after injection. Image from Ohtsuka et al., 2018...

...efficiently transduce your cells. In fact, we usually recommend testing more than one.  Most AAV serotypes have...the amount of virus you add, so it is strongly recommended to optimize to the “lowest number required” instead...reading! Good luck, and happy optimizing!  Recommended Reading Issa, S. S., Shaimardanova, A. A., Solovyeva...

...mentorship relationships are valuable and highly recommended, a mentor with international experience provides... opportunities that truly exist outside their immediate frontiers. Moreover, sharing a culture/country...and advisor. For their own part, mentors receive immense personal gratification as they witness their student...

...the past few years to propose standards and recommendations for improving antibody use and reliability ... al., 2016). The major takeaway from these recommendations is: validate, validate, validate. This doesn.../19420862.2015.1100787  Taussig MJ, Fonseca C, Trimmer JS (2018) Antibody validation: A view from the ...

... to provide letters of reference, informal recommendations, more positive grant application and manuscript... active learning. Serve as a daily mentor for a summer undergrad, rotating grad student or high school...your perspective like intense exercise. I highly recommend running or biking or something that demands your...

...systems Tag Size (amino acids) Ligand Commercial ligands available Ease of use Tetracysteine...possibilities and save money). There aren’t as many commercial options for CLIP-tag and very few TMP-tag or ...Ohana, R. F., Urh, M., Simpson, D., Mendez, J., Zimmerman, K., Otto, P., Vidugiris, G., Zhu, J., Darzins...

...viral titer High vector genome (vg) doses are recommended for all systemic capsid applications. For example... elements, etc... *These are only starting recommendations; you will still need to experimentally determine...found a good fit (or if you need more options) we recommend you check out Caltech’s CLOVER Center’s extensive...support and input. Resources and references Recommended reading Challis R.C., Ravindra Kumar S., Chen...

... you probably know as TaqMan) and dsDNA dyes (commercial name: Sybr). However, there are other chemistries...here are some general helpful principles (and I recommend Bustin, 2017 for a more in-depth review.)  Your...normalization of signal. They are often included in commercial sets and most machines have a protocol that automatically...

...antibody, especially a validated antibody, we recommend using antibody search engines and antibody data...search engine that you use? Let us know in the comments so we can add it to the table! And if you're interested...

...plasmids on Addgene’s site (Side note: I always recommend you read the article to ensure you understand ...even easier and more useful, let us know in the comments. I hope this helps! Additional Resources on the...

...put this technology into a concrete context. I recommend this post to anyone wondering if and how they ...Order-dependent Logic in vivo As always, feel free to comment on any of our posts if you have any questions or...

...pBACKBONE-XXXXX Tip: For a catalog of published and commercially available empty backbones, visit Vector Database...letter abbreviation of the wild type amino acid immediately followed by its position (number) relative to...

...career options in science, with Abigail Anderson commenting that “There is not just a scientist or a professor...Let us know about your educational events in the comments section below! Additional Resources Visit the...

...transform your plasmid DNA If you decided to use commercial competent cells, then you're best off following...of competent cells with those obtained using commercially available cells or previous batches of homemade...

...website. 65 audience members gave up a beautiful summer Saturday to learn about science, but they didn’...efforts to engage the public - let us know in the comments section! More information about SITN: DayCon...

...Historically we do better in September than in the summer months, but this is also our best month ever! Hats...to mention any apps you’ve found useful in the comments section to make this post even more useful to ...

...Addgene Executive Director Joanne Kamens. I highly recommend taking a look at all of our posts as each one ... questions or suggestions for us, feel free to comment below. Happy reading! Tyler J. Ford ...

...Joanne's Reddit AMA Here they are annotated with my comments: Everyone on the team talks and listens in roughly...informal gatherings, like at lunch. Many experts recommend using anonymous surveys with regular frequency...

...and high-profile universities. We particularly recommend Eric Lander's intro to biology course "The Secret...OA developments of the past year with us in the comments. Or let us know your favorite ways to share science...

...going on trips, and going to New Hampshire in the summer for the annual lab retreat. How do you manage work-life... aren’t locked into a schedule.” George Church comments on how he would go home in the afternoon during...

...Addgene account.  Orders do not need to be placed immediately upon request approval. Once approved, the request...cannot perform functional or in vivo testing. We recommend you check the original paper of your AAV plasmid...

...Tom Ellis 1. Keep up with the literature. He recommends setting up a few Pubmed email alerts for new ... have goals outside of the university? Ellis recommends that a student interested in leaving academia...

...protein of interest. Many of these tags also have commercially available antibodies, resins, beads, and other...can be removed via protease cleavage at sites immediately following the tag. Unlike MBP, the GST tag can...

...for plasmids assembled from publications and commercially available sources, as well as those deposited... not listed in today’s post? Share them in the comments below. This post was written in April 2020 by ...

...camelid-derived single-domain antibodies (sdAb), commercially known as nanobodies (Arbabi-Ghahroudi, 2017)...antibody and single-domain antibody, under the commercial "Nanobody" name.  We’ve talked about sdAbs...

...seminars exposes you to discussions beyond your immediate field, fostering a diverse range of perspectives...current project. Another strategy could entail immersing yourself in diverse literature. By delving into...

...correct wavelength of light. This response is immediate, on the scale of milliseconds, and can precisely... As a result, it is not possible to elicit an immediate response or track the exact time of activation...

...society also organises PhD information events and summer internship information sessions to help undergraduate...provide during your education? Let us know in the comments section below! Do you know of a society or event...

...section on a plasmid webpage for any specific recommendations from the depositing lab. If your vector is ...recombined product tend to grow faster, so we recommend picking the smaller colonies which are more likely...

...and releasing topoisomerase (2).  These days commercially available TOPO kits provide vectors or cloning...When incubating at room temperature, it is not recommended that you exceed the 5 minute time limit (lower...