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Showing: 41 - 60 of 173 results

  1. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...few steps. More recently, CRISPR technology has adapted Golden Gate cloning for inserting the appropriate...single gRNA-expressing plasmid, but it can also be adapted to express multiple gRNAs. Addgene has two Golden...

  2. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...polyadenylation (spA) signal. Figure and caption adapted from Hazan and Bester 2021.    Alternative ...Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity. Science. 2012;337(6096):816...

  3. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ... the simplest CRISPR systems and the easiest to adapt to genome editing, but the introduction of type ...positions are indicated below each graph. Figure adapted from Kleinstiver et al., 2016. Until recently...

  4. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...MagLOV’s parent protein AsLOV2 has already been adapted into a variety of optogenetic tools, they’re optimistic...optimistic that MagLOV can be similarly adaptable into future magnetogenetic tools.  Embracing science...

  5. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...interference (CRISPRi) has been limited to lab-adapted bacteria because it has been challenging to introduce...for using CRISPRi in many types of bacteria: lab-adapted, environmental isolated, and disease-associated...

  7. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...spreading within and between genomes, and have been adapted for a wide variety of applications - as mutagenesis...at two different incubation temperatures. Data adapted from Vo et al., 2020. In E. coli, the new streamlined...

  8. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...applications. The first non-Cas9 CRISPR protein adapted for genome engineering was Cas12a (formerly Cpf1...targeting flexibility. Cas13a (formerly C2c2) has been adapted for targeted RNA cleavage in mammalian cells. The...

  10. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...trafficked from the “source” to “destination”. Images adapted from Lee and Cheah et al. 2023 bioRxiv (A) and ...:GFP-iPAK4 at 3 or 6 h. Scale bar 5 µm. Images adapted from Lin et al., 2023 bioRxiv, under CC-BY-NC 4.0...

  11. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...have been developed for a singular purpose (ex. adapter trimming, quality score analysis, k-mer counting...Java, R, etc.) in our pipeline. Crucially, this adaptable property gave us the freedom to use multiple open-source...

  13. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...efficiencies at different loci in U2OS cells. Image adapted from Yan et al. 2024 under a CC-BY license. ...transduction efficiency for the indicated ssAAV5s. Figure adapted from Serrano et al. 2023 under a CC-BY-NC-ND license...

  14. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...the late 1980s, scientists even created a codon adaptation index (CAI) that is based on the codon usage ...10.1038/nrg3051 Sharp PM, Li W-H (1987) The codon adaptation index-a measure of directional synonymous codon...

  15. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...structure of the H1.8-GFP-bound nucleosome. Images adapted from Arimura et al. 2024 under CC-BY-NC-ND 4.0 ...3D map with 3.1 Å global resolution (E). Figure adapted from Abe & Lim, 2024 under CC-BY-NC-ND 4.0 license...

  17. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ... culture, and also needed to be resourceful and adapt. By the end of my three months, I fully understood...companies which have more bureaucracy and cannot be as adaptive. I was also surprised to learn that I enjoy ...

  18. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...recombinant plasmid construction (restriction cloning), adaptor ligation for next generation sequencing (NGS) library...PMCID: PMC1978145. 27. Greenough, Lucia, et al. "Adapting capillary gel electrophoresis as a sensitive, ...

  19. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...black is the fusion tag or labeling system. Image adapted from Lavis, 2016, under CC-BY. Common self-...comparison with standard confocal microscopy. Image adapted from (Bottanelli et al., 2016) under CC-BY license...

  20. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...codons, and/or the phenomenon of transcriptional adaptation (Smits et al., 2019; Sztal and Stainier, 2020...Sztal TE, Stainier DYR (2020) Transcriptional adaptation: a mechanism underlying genetic robustness. Development...
Showing: 41 - 60 of 173 results