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We narrowed to 79 results for: Blue fluorescent Protein

Showing: 41 - 60 of 79 results

  1. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...direct fusion of fluorescent proteins (FPs) to a yeast gene of interest. These fluorescently tagged genes ... localization of individual proteins, but also investigate protein-protein interactions via FRET. Sidae... especially useful in protein complex studies where tagging of multiple protein products is desired, as... unique protein fusions. This collection is ideal for tandem affinity purification of protein complexes...advantage of this natural process to integrate protein tags into the genomes of S. cerevisiae and S. pombe.... A given insert will typically contain both a protein modification sequence and a selectable gene product...several ready-to-use, modular plasmids, combining fluorescent tags, epitope tags, protease sites, and selection...

  2. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ... the Fluorescent Protein Featured Topic Page Resources at Addgene.org Find Fluorescent Protein Biosensors...other Fluorescent Biosensors A Practical Approach to Choosing the B(right)est Fluorescent Protein Visit...periplasmic glutamate binding protein known as GltI. When the resulting fusion protein binds to glutamate, conformational...Biosensors Browse All Fluorescent Proteins Find AAV Tools References Marvin, Jonathan S., et al. “An optimized...conformational changes lead to an increase in fluorescence intensity from the inserted GFP. After characterizing...expression levels in bacteria and produces stronger fluorescent signals upon sensing glutamate in mouse and ferret...glutamate), that came in three different colors – blue (SF-Azurite-iGluSnFR), green (SF-iGluSnFR), and ...

  3. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...version at Addgene! Fluorescent protein and control AAV These fluorescent protein expression vectors can...exhibits high contrast between peak fluorescence and resting fluorescence. It is useful for activity imaging...exhibits high contrast between peak fluorescence and resting fluorescence.  It is useful for activity imaging...pGP-AAV-syn-FLEX-jGCaMP7b-WPRE) exhibits the brightest resting fluorescence of the GCaMP7 family of calcium sensors and ...the endoplasmic reticulum, but exhibits larger fluorescent responses to GABA. Find the iGABASnFR and its...optogenetic activators and inhibitors that respond to blue light. We recently released a constitutive (pAAV-Syn-Archon1...the control vectors for experiments encoding a fluorescent-tagged molecular tool.   Find pAAV-FLEX-tdTomato...

  4. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...tags to your gene, and may have even put a fluorescent protein downstream, separated by an IRES element....galE15 galK16 rpsL(StrR) endA1 λ- XL1 Blue Tetracycline Blue/white screening and routine cloning, nalidixic...companion post describing protein expression strains, where we go over the basics of protein expression in E. ...general lab strains designed for subcloning or protein expression. If you were to include customized strains...gene Increases the stability of certain expressed proteins endA, endA1 Endonuclease I (nonspecific cleavage...fertility plasmid. A low copy-number plasmid, encodes proteins needed for bacterial conjugation. Genes listed... cannot methylate DNA lac Lac operon mutations Blue/white screening of clones   lacIq lac repressor...

  5. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...provided by red fluorescent proteins such as tdTomato or RFP or blue fluorescent proteins like Cyan. The... David W. Piston. "Fluorescent protein tracking and detection: fluorescent protein structure and color...their fusion to a POI can affect protein folding and protein-protein interactions. Also, overexpression...have created antigenic protein tags called “spaghetti monster” fluorescent proteins (smFPs). smFPs are literally...Neuronal tracers such as epitope tags and fluorescent proteins (FP) have also been important in mapping...With the limitations of epitope tags and fluorescent proteins, it has been crucial to develop new tracers...intrinsically fluorescent and can be detected with highly epitope-specific, fluorescently-labeled antibodies...

  6. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...DNA Fluorescence dyes Another way to quantify DNA would be to use fluorescent dyes that fluoresce when...and their corresponding fluorescence. That way, you can compare the fluorescence of your sample against...acids (DNA and RNA) absorb maximally at 260 nm. Proteins on the other hand absorb best at 280 nm and organic...sequencing. Unlike absorbance-based methods, fluorescence-based methods require a standard curve, a set...this method doesn’t tell you about contaminating proteins or chaotropic salts. Capillary electrophoresis...These fragments are quantified over time using a fluorescent dye that intercalates into the DNA such that ... using capillary electrophoresis which shows fluorescence units over time. Smaller fragments migrate more...

  7. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...the available green fluorescent proteins emit light. The pKa of fluorescent proteins can be accurately ...use of bright red fluorescent proteins (e.g. mScarlet) or infrared fluorescent proteins (Chernov et al.,...Introduction to GFP Read our Fluorescent Proteins Blog Posts Download the Fluorescent Proteins 101 eBook Resources... Browse the Fluorescent Protein Collection Find empty backbones for fluorescent protein fusions Browse...co-factor binding proteins, systems wherein a protein binds a synthetic, fluorescent analogue have been...for another protein. Effectively, it equips the protein of interest with an additional protein module that...Structural Dynamics of Green Fluorescent Protein Fused with a Single Chain Fv Protein." Journal of Biological...

  8. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...cyan-excitable red fluorescent protein (mCyRFP1). Derived from cyan-excitable organge fluorescent protein (CyOFP1...on This New AAV Tool    mCyRFP1: Novel fluorescent protein that enables imaging of two biosensors simultaneously...as a donor in FRET (fluorescence resonance energy transfer) and FLIM (Fluorescence Lifetime Imaging Microscopy...Podcast Segment New bright monomeric red fluorescent protein - mScarlet! The Dorus Gadella lab has recently...recently developed a synthetic red fluorescent protein variant they call mScarlet. Bindels et al used a...evolution of new RFP variants The toolbox of fluorescent proteins (FP) for cellular imaging is constantly ...larger strategy to engineer improved red fluorescent proteins (RFPs) and, while the authors note that ...

  9. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...epitopes fused to the protein of interest are recognized and thus fluorescently labeled by scFv antibodies...works by bringing together an anchor protein and activator protein to drive transcription. The anchor consists... Upon stimulation with blue light, the complex recruits a CIB1 binding protein (a portion of CRY2) fused...off all mRNA binding proteins as it travels along the mRNA to produce the protein product. The system,...RNA Imaging by Coat protein Knock-off, is made up of three components: a GFP protein that binds to the ...of a reporter mRNA via a PP7 coat protein (NLS-PCP-GFP) a RFP protein that binds to the 3’ UTR of the reporter... sites for the fluorescent proteins and an inducible promoter (Plasmid 64543or 64542) When the reporter...

  10. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ...GFP reporter that is converted into BFP (blue fluorescent protein) upon CRISPR/Cas9 homology directed repair...GO works by affecting protein translation of different reporter proteins. Protein translation requires ...the translation of any detectable protein, not just fluorescent proteins. Figure 4: Reporters in Gene...initiate protein expression, it can be used for an array of detectable proteins including fluorescent proteins...insertion that disrupts fluorescence due to a frameshift. Restoration of fluorescence can only occur through...monitors APOBEC (a family of cytidine deaminase proteins) and Cas9-mediated editing in real time (St. Martin...mCherry expression. Cells can then be sorted using Fluorescence-activated cell sorting (FACS) and the editing...

  11. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...constitutively express a red, green, or blue (RGB) fluorescent protein (4a-c). When high doses of this viral...are easy to trace and not lost in a jumble of fluorescent neurons. A simple but flawed approach to multi-color...transduced by only one RGB AAV and red, green, or blue are the predominate colors expressed (figure 4c)...A cocktail of three AAV vectors expressing RGB proteins under the control of an inducer, i.e. tet-off ...morphology studies when used as part of a two part fluorescent tag-then-express system that allows for controlled...

  12. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ... blog Fluorescent Proteins 101: Fluorescent Biosensors Resources on Addgene.org Fluorescent protein...short), where a fluorescent protein is inserted into one of the loop regions of any G-protein-coupled receptor...NIR calcium reporter Near-infrared (NIR) fluorescent proteins promise better performance for in vivo use...calcium indicator using small monomeric NIR fluorescent proteins (miRFP670nano and miRFP720) and the Ca2+...engineering of potassium binding proteins, they developed a family of fluorescent potassium indicators with ...requirements (Lu et al. 2022). By fusing two bright fluorescent proteins with different thermal quenching rates (...protein plasmids and resources Fluorescent protein guide: Biosensors Collection ...

  13. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type
    Blog Post
    Published
    June 6, 2019, 12:41 p.m.
    ...containing two fluorescent proteins and four repeats of a miRNA target site. One fluorescent protein (EBFP2) ...B (BoNT/B), which cleaves the synaptic protein VAMP2, a protein essential for synaptic transmission. Cleavage...pair of proteins (iLID and SspB) that undergo light-induced dimerization. Upon application of blue light...and function of microbes rely heavily on fluorescent protein genes. These optical reporters have limited...gas-filled protein structure that water-bound photosynthetic organisms use for buoyancy. These proteins allow... lab discovered in 2014 that these gas vesicle proteins scatter sound waves and create ultrasound contrast...

  14. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...tool that controls nuclear localization (LINuS). Blue light renders the NLS accessible and the complex...stem cells Lindy Barrett’s lab developed new fluorescently labeled piggyBac vectors to deliver uniform ... lines with AAVS1-integrated, inducible, and fluorescent dCas9-KRAB and dCas9-VPR for quantifying and ...that express both the dCas9 construct and the fluorescently-labeled gRNA multiplex. Read the paper in Scientific...methylcytosine editing by TET1 by co-delivering TET1 and protein factors for linking methylcytosine oxidation to...

  15. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...make. For example, large insertions, like a fluorescent protein knock-in, suffer from low efficiency when... your desired insertion feature (e.g., a fluorescent protein), you can amplify the desired region using... of the commonly used cassettes, such as fluorescent proteins (EGFP, mCherry etc.), recombinases (Cre,...purify Cas9 protein. The table below contains some of our favorites, all of which have blue flames. ... Quadros et al. recommend ribonucleoprotein (RNP) complexes of Cas9 protein and the separated guide RNA...options for templates - see the example at right using blue flame plasmid pmScarlet_C1! After creating the T7...alleles using long ssDNA donors and CRISPR ribonucleoproteins.” Genome Biol. 18 (2017) (1):92. DOI: 10.1186...

  16. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...when illuminated with blue light, catalyzing the biotinylation of nearby proteins. B) TransitID uses sequential...locations. C) Enrichment of dual-labeled proteins identifies proteins that had been trafficked from the “source...generated a fusion protein called iPAK4 that assembles into a slow-growing protein fiber in the cytoplasm... Figure 4: Time-resolved protein ticker tape. A) Design of iPAK4 protein ticker tape recording. B) HEK...labeling and TransitID Tracking cellular history with protein ticker tape Teaching an old antibody new tricks...recently used phage-assisted continuous evolution and protein engineering to generate seven new prime editors...., et al. (2023). Phage-assisted evolution and protein engineering yield compact, efficient prime editors...

  17. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...photostable than fluorescent proteins, making them ideal for single-molecule fluorescence techniques. After...CLIP-SNAP fusion proteins for single-molecule FRET. A) Schematic of a CLIP-SNAP fusion protein (a.k.a. CLAP... a flexible linker where a protein of interest can be inserted. B) Protein expression and labeling procedure...optimized strategy uses new combinations of PE protein components (Figure 1), an altered epegRNA delivered...study the conformational dynamics of the motor protein kinesin-1, the Twelvetrees Lab took advantage of...FRET to study the intramolecular dynamics of the proteins in solution and when associated with microtubules...generating other SNAPf-, CLIPf-, and CLAP-tagged proteins for mammalian cell expression. These plasmids ...

  18. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...MitoTimer proteins are derived from the Timer protein - a mutant of the fluorescent protein dsRed that...three possible fluorescent proteins, chosen for minimal spectral overlap, minimal protein aggregation, ...groups to monitor protein and cell ageing, as the spectrum shift occurs during Timer protein life due to a... synthesized (green) protein and organelles with older (yellow to orange) protein. Image courtesy of Roberta...1852-61. A quicker method for protein inactivation Your protein of interest does x, but it also participates...your endogenous protein of interest and co-expressing its replacement recombinant protein with a FKBP domain...outer membrane protein with a FRB domain (MitoTRAP). Rapamycin binds your recombinant protein (via FKBP) ...

  19. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...can visualize the protein via fluorescence or chemiluminescence, precipitate the protein out of solution... a single protein that can recognize the target protein (Wang et al., 2013). These proteins are also plasmid-derived... the presence of proteins from samples containing a mixture of proteins. The proteins are separated based...to study protein-protein interactions. Flow cytometry Flow cytometry is used to detect proteins on the ...scientists to target specific proteins they’re interested in. Once a protein is targeted with an antibody...entirety of the target protein: instead, it recognizes a small portion of the protein, known as an epitope... the target protein because the antibodies will recognize multiple epitopes on the protein. However, each...

  20. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...determine how Protein A and Protein B interact. You read extensive research on the two proteins and come up... an ideal antibody against Protein A and an ideal antibody against Protein B. The antibodies are both ...to detect Protein A and green fluorophore-tagged anti-mouse IgG2a secondary to detect Protein B and successfully... anti-Protein A antibody and an anti-mouse IgG2a secondary antibody to bind to the anti-Protein B antibody...process the variable regions of a parental antibody (blue, rat) are cloned into a backbone expressing the ...are rat IgG1, then you could convert your anti-Protein B antibody to a mouse IgG2a Fc. The resulting chimeric...successfully visualize both proteins.     Figure 2: Two primary antibodies with the same isotype...
Showing: 41 - 60 of 79 results