...photoswitches Photoactivation of Cas9 can also be achieved using single-chain fusion proteins by fusing Cas9...was from the lab of Michael Lin, who developed a chimeric protein consisting of Cas9 and a photo-dissociable...control over Cas9 activity. Dominik Niopek’s lab achieved this by fusing the anti-CRISPR Acr protein, AcrIIA4...resulting system was coined as CRISPR–Cas9 activity switching via a novel optogenetic variant of AcrIIA4 (CASANOVA...-mediated CRISPR labeling to study chromatin architecture (Hoffman et al., 2020). Fluorescent dCas9 proteins...can potentially alter the genome and chromatin architecture, it is useful to regulate the time in which ...tRNA synthetase pair which would add the PCK upon reaching the amber stop codon, TAG. (To learn more about...

...that you come into the lab wishing there was a machine that could make your life a hundred times easier...off our hands. Good news! There is probably a machine or robot out there that does exactly what you want...lot of time. Enter the Biomek FXP! We wanted a machine that would improve our transformation protocol ...played a factor in our decision to go with this machine, but we also really liked that Beckman was flexible...apart before plating. With this fine piece of machinery we have been able to transform 96 samples in about...lab team. Enter the Liquid Handler!     This machine fits on a table top and can be easily programmed...t electronic but it is one of the most helpful machines I have used in the lab. It’s especially useful...

...about how I could combine all of these roles by reaching out to my school district not just as a dad, but...presentations. If you’re a scientist thinking about reaching out to your community’s schools, here are a few...recent experiences. STEM in elementary schools - catching them early I’m fortunate to live in a town with... means to request funding for events, consider reaching out to it. PTAs have more flexibility than the...but I also tried not to be an alarmist.  The overarching guideline for all of these questions, and for...community as a scientist. Perhaps you have young children and you want to make sure their STEM education...regardless of race or ethnicity. Perhaps you don’t have children but are interested in testing your SciComm skills...

...CRISPR-Cas9-assisted genome editing in Escherichia coli. Acta Biochimica et Biophysica Sinica. https://doi....Yang, a professor in synthetic biology from the Chinese Academy of Sciences. In the Yang lab, we’re continually...dehydrogenase and generated a strain that could achieve glucose dehydrogenase expression that is 2.6 times...guest bloggers! Qi Li obtained her Ph.D. from the Chinese Academy of Sciences (CAS) Center for Excellence...Ph.D. from Shanghai Institute of Biochemistry, Chinese Academy of Sciences. He joined Shanghai Institutes...Yang J, Yang S (2015) Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System. Appl ...

... in China. They were also able to help with sharing information through the use of popular Chinese social...have partnered with six distributors (Table 1), in China, Korea, Japan, Brazil, India, and Mexico. Table ... Country Served Beijing Zhongyuan 2012 China Summit Pharmaceuticals International 2013 ...agreement with a distributor for that country. In China, direct shipments weren’t able to clear import control...In 2023 alone, viewers spent over 22,000 hours watching these and other videos on Addgene’s YouTube channel... We’ve learned that increased accessibility is achieved through interconnected incremental changes. It...

...organisms!) Escherichia coli One of the most recognizable and utilized model organisms is Escherichia coli. ...introductory teaching and research environments (Idalia & Bernardo, 2017).  Escherichia coli. Image...2017). Escherichia coli as a Model Organism and Its Application in Biotechnology. In Escherichia coli -...used mammalian model in medical research with an archive of information and data that has yet to be produced...

...afternoon during the child-rearing years, learning “lots of interesting things from children, their classmates...work-life balance. How do you celebrate your lab’s achievements? Tom Ellis highlights the importance of acknowledging...Although hard work and perseverance are required to achieve success in the lab, it is important to take the...along the way. Take time to celebrate the lab’s achievements and enjoy time with friends and family. And ... this series about how they manage their labs, achieve success, and have fun with their lab. Further ...

...Hill University of Illinois Chicago                     Boston Children's Hospital                           ...fighting the pandemic could be even more impactful by reaching more scientists. Many members of the scientific...industry-based scientific community. Conversations with children playing in the background became a normal occurrence...Institute                                       Chinese Academy of Medical Sciences University of Texas...plasmids quickly and Addgene’s quality control and archiving system allowed us to handle and distribute such...

... and were only available as green probes. The Patriarchi Lab aimed to overcome these limitations by developing...researchers looking to study norepinephrine, the Patriarchi lab continues to work on further improvements...nLightG. Many thanks to Mike Waring and Tommaso Patriarchi for their assistance with this blog post. References...and resources References Feng, J., Zhang, C., Lischinsky, J. E., Jing, M., Zhou, J., Wang, H., Zhang, ...Benke, D., Weber, B., Bohacek, J., Reiner, A., … Patriarchi, T. (2023). Sensitive multicolor indicators for...Mirdita, M., Schütze, K., Moriwaki, Y., Heo, L., Ovchinnikov, S., & Steinegger, M. (2022). ColabFold: Making...

...experiment. Nucleic acid based machines Recombinase-based State Machines Enable Order-dependent Logic in... cell. These so-called recombinase-based state machines could potentially be used to report upon and/or...post to learn more about recombinase-based state machines and how they function. Adapting Toehold Switches... describes R-bodies, “force generating protein machines that can extend and retract over many microns ...you can better design your donor DNA template to achieve more efficient genome editing - all influenced ...

...relationships, so that you can reap rewards in the form of reaching career development goals. Check out Joanne's Reddit... adjust expectations, and record milestones in reaching goals. The more time and thought you put into ...setting goals, the more progress you will make in reaching them. Of course, you won’t reach all the goals...happens. Mentors bring more than their knowledge and teaching to help a Mentee reach goals. One of the most ...sometimes it is only the knowledge that they will be watching out for me that gets me out of bed and into my...being a Mentor: Learn other perspectives Develop coaching skills Encourage your creative thinking Enhance...

...specificity far greater than the one that can be achieved using pharmacological or electrophysiological ...., 2016; Hudry and Vandenberghe, 2019). How to achieve strong opsin expression in specific neuronal cell...expression in vivo (Schnütgen et al., 2003). This is achieved through site-specific recombinases such as Cre...expression in specific neuronal cells? This can be achieved using Cre-dependent viruses, such as the optogenetic... functional topography of the neural circuit architecture of the brain regions associated with motivated...love for educational science led her to pursue a teaching career, and she now is a very happy scientific... and approaches for optogenetics." Biological psychiatry 71.12 (2012): 1033-1038. PubMed PMID: 22480664...

...traditional cell culture, enriching your active RNAi subpopulation can be easily achieved through fluorescence-activated...resulting in processing by the endogenous RNAi machinery. Alternatively, endogenous miRNA precursors can...initial state of the dsRNA upon activating the RNAi machinery (long dsRNA, typically hairpin RNA, or artificial...gene target to silencing of the reporter. This is achieved at the level of the DNA construct, where your ...itself is indicative of active gene silencing. We achieved this by creating constructs that directly fused...Polytechnic Institute where he investigated how plants achieve polarized cell growth in the lab of Luis Vidali...

...gRNAs are released by endogenous tRNA processing machineries, specifically, RNaseZ and RNaseP. Although the... for gene editing. They named this new array architecture the DAP array, short for “drive-and-process....length of 5’ leader sequences of a given tRNA to achieve the highest efficiencies of multiplex editing at...also engineered a DAP array for prime editing, achieving up to 3-loci MPE with similar high efficiencies...payloads. Different from existing multiplex array architectures, including previous tRNA-gRNA arrays, that rely...Comparison of previous representative dual-gRNA architectures with DAP array   Qichen Yuan is a PhD...

...than KillerRed, with KillerRed reaching greater than 75% photobleaching after just 1 minute of exposure...produce ROS - targeted cell ablation via ROS is achieved through localized expression of the FAP. A new...portion of the complex and is derived from the malachite green (MG) dye, but has been modified with the...longer exposure times (5 minutes vs 10 seconds) to achieve the same level of killing as Mg-2I-dL5**. Mg-2I-dL5...exposure. MG-2I-dL5** experienced minor bleaching after longer exposure times. Additionally, KillerRed requires...

...provide them on your smart phone. 1. Lab Archives by LabArchives The single, most important part of lab ... life. These apps prevent you from constantly searching the interwebs for lab resources, and instead conveniently...lab life is the lab notebook. Lab Archives is a multipurpose application that allows you to share your data...notebook from your smartphone to your computer. Lab Archives will help both you and your lab stay organized...from 2 years ago. The free subscription to Lab Archives allots for 25 MB of space per user, but, if you...

...What is an Antibody animation!  Isotype switching Isotype switching occurs when a B cell changes which heavy...other isotype heavy chain constant domains. This switching helps B cells adapt during the course of an immune...the effector function of the antibody. Isotype switching is caused by a specialized type of DNA recombination...IgG are deleted.     Figure 2: Isotype switching requires DNA recombination of the antibody gene...constant domain gene. This process is called isotype switching and results in the B cell expressing an antibody...

...immensely. Paratore: I have two children active in sports, so I enjoy watching their games.  If I’m not at....Paratore: I’ve certainly had a front row seat watching the international growth through my work on the...graduated with highest honors from the University of Michigan, Ann Arbor and obtained her J.D. from Boston University...CISA), and certified health insurance executive (CHIE). He is a member of both the Internal Institute ...Food Bank and participates locally in Read To A Child's "Lunchtime Reading Program" in Cambridge, MA. ...

...-based policy and legislation hit home hard in Michigan, where programs like the Great Lakes Restoration...planet. And as we rallied on the University of Michigan campus speakers thoughtfully reminded us to use...a postdoctoral researcher in the University of Michigan Department of Astronomy. She studies how planets...tirelessly to get the publications, grant funding, and teaching experience that it takes to complete your resume...march ended that I loved - there were booths for children to learn little things about science. We need ...to be the perfect opportunity to cement in my children's heads the importance of science and positively... that most who participated will consider that marching in and of itself will be enough. IT WON’T! Promoting...

...transcription machinery.   Well, I’ve got some good news: complete DNA demethylation can be achieved by dCas9...can be achieved by fluorescence-activated cell sorting, and stable gRNA expression can be achieved using... activity. They interact with transcriptional machinery to modify local gene expression, confounding any...doi.org/10.1016/j.neuron.2013.08.032.  Morita S, Noguchi H, Horii T et al. (2016) Targeted DNA demethylation...