We narrowed to 79 results for: CHL

1. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

   Type

   Blog Post

   Published

   Sept. 3, 2019, 1:15 p.m.

   ...optogenetic toolbox Article contributed by Susanna Bachle Anion-conducting channelrhodopsins (ChR) are ion...

2. Summer SciComm Series: A PhD in Science Communication

   Type

   Blog Post

   Published

   Aug. 23, 2022, 1:15 p.m.

   ...poring over my grandparents’ medical textbooks by torchlight under the covers. In high school, I went to all...

3. Plasmids 101: Gram Negative and Positive Bacteria

   Type

   Blog Post

   Published

   Aug. 2, 2022, 1:15 p.m.

   ...types. In water, the dye dissociates into CV+ and Chloride (Cl-) ions. When the negatively charged iodine...

4. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

   Type

   Blog Post

   Published

   Nov. 27, 2018, 2 p.m.

   ...By Susanna Bachle and Ina Ersing Seeding Labs and Addgene collaborate to provide scientific resources...

5. R Bodies: Membrane-Rupturing Microscopic Tools

   Type

   Blog Post

   Published

   April 14, 2016, 2:30 p.m.

   ...Publication at Addgene 4. Petroni, G., Spring, S., Schleifer, K.-H., Verni, F. & Rosati, G. Defensive extrusive...

6. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

   Type

   Blog Post

   Published

   June 25, 2019, 12:54 p.m.

   ...in the lab. This is commonly done using calcium chloride which permeabilizes the cell membrane so the bacteria...

7. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

   Type

   Blog Post

   Published

   July 6, 2017, 1:59 p.m.

   ... Susanna Bachle...

8. Multiplex Genome Editing with CRISPR-Cpf1

   Type

   Blog Post

   Published

   May 9, 2017, 2:12 p.m.

   ...Abudayyeh, O., Slaymaker, I., Makarova, K., Essletzbichler, P.,  Zhang, F. (2015). Cpf1 Is a Single RNA-Guided...

9. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

   Type

   Blog Post

   Published

   March 17, 2020, 1:32 p.m.

   ... Susanna Bachle...

10. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type

    Blog Post

    Published

    Jan. 30, 2020, 2:15 p.m.

    ...is in citizen science and behavioral ecology of cichlid fishes. Find David online at https://orcid.org/...

11. Antibodies 101: ChIP

    Type

    Blog Post

    Published

    March 10, 2022, 3:25 p.m.

    ... with a PCR purification kit or with a phenol-chloroform extraction.    Fig. 1: Schematic overview...

12. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type

    Blog Post

    Published

    Dec. 6, 2018, 1:34 p.m.

    ...active LacZ tetramer.  This tetramer cleaves chlorophenol red-b-D-galactopyranoside on the assay paper...

13. Addgene's Tips for Plasmid Quality Control

    Type

    Blog Post

    Published

    Jan. 14, 2016, 3:30 p.m.

    ...expected full plasmid sequences (examples include Benchling, Serial Cloner, and Snapgene), and save these ...

14. Working with Nuclear Receptors

    Type

    Blog Post

    Published

    July 15, 2014, 4:03 p.m.

    ...reporter gene has over the years evolved from chloramphenicol acetyltransferase to luciferase, the basic ...

15. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type

    Blog Post

    Published

    July 24, 2018, 12:51 p.m.

    ... Susanna Bachle...

16. Adenoviral Vector Production and Troubleshooting

    Type

    Blog Post

    Published

    June 14, 2018, 11:44 a.m.

    ...standard method for purification of rAdV uses cesium chloride (CsCl) density gradients combined with ultracentrifugation...

17. Viral Vectors 101: Chemogenetics

    Type

    Blog Post

    Published

    July 11, 2023, 1:15 p.m.

    ...Roth's and Takafumi Minamimoto's lab called deschloroclozapine (DCZ) shows promise as a CNO alternative ...

18. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type

    Blog Post

    Published

    May 7, 2020, 1:15 p.m.

    ... Susanna Bachle...

19. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    ... apramycin, blasticidin, bleocin (zeocin), chloramphenicol, erythromicin, gentamicin, hygromycin, kanamycin...

20. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    Published

    July 14, 2016, 2:30 p.m.

    ...JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev ...