...also ideal for signal amplification during these experiments. During detection, a biotinylated reporter...can be added to either terminus of a DNA sequence during a Polymerase Chain Reaction (PCR) by using a biotinylated.... Biotin can also be enzymatically incorporated during PCR using a biotin labelled dUTP. There are multiple...

... tends to be slightly more popular due to its durability and high binding capacity. In addition, PVDF ...blocking the membrane  from binding to the antibodies during the stain. The ideal blocking buffer depends on...common readout for subcellular fractionations, a procedure that isolates proteins from different cellular...polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National...

...agility as a nonprofit resource can prove to be vital during this period of constrained funding. Addgene is ...partner for archiving their biological materials during funding transitions. Enabling resource sharing:...what support would be most valuable from Addgene during this time. Your input will directly shape our response...

... contaminants that have been used during the purification procedure. Good indicators of DNA purity are...guanidine thiocyanate and guanidine hydrochloride used during plasmid extraction. Figure 2: Use cloning...

...of modified Ti plasmids during plant infection, as well as plasmid yield during preparation in E. coli....Agrobacterium to ensure that they are maintained during co-cultivation with the target plant species. Thus...

...were inspiring! We also got to hear more from them during a panel discussion with Dr. Craig Mello and Dr....enough to make up for the somewhat naïve questions. During the panel, an artist was sketching her interpretation...accelerate the launch of clinical applications. During the panel discussion, Dr. Mello took it upon himself...

...Wisconsin lab for a summer during my PhD and is responsible for my enduring love of cheese curds. We’...distribution service! Did you see a must-have plasmid during ASM? Let us know what’s missing in the collection...

... agar slices.  Molecular biologists used this procedure daily before the advent of techniques like PCR...temperature (thus also saving freezer space).  The procedure becomes less attractive when noting that one step... had to be careful not to stick your fingers in during a run or risk minor electrocution.  These days ...

...MCS/SacI site in pUCXKT, removing the stop codons during backbone digestion and providing the missing codons...utilizes the bulldozer-like quality of the ribosome during translation, which knocks off all mRNA binding ...both the GFP and the RFP proteins in the nucleus. During the first round of translation, the GFP proteins...exported to the cytoplasm. Furthermore, they find that during stress, mRNAs in P-bodies are translationally repressed...now follow movement of two or more targeted loci during the cell cycle or cell differentiation. This tool...observe intra- and post-Golgi segregation of cargo during their transport. Finally, the system can be adapted...

...are able... Thanks to everyone for being awesome during a tough time. We're all going to get through this...Addgene Team because they’re all doing amazing work during this difficult time. One function that rose to ...had to figure out a way to maintain our services during the crisis and maintain our workforce for when ...

...restriction enzymes or ligase, and requires no post-PCR procedures. Sounds easy right? The technique relies on the...insert, the magic of TOPO cloning happens. Basic procedure Let’s break down the steps needed for TOPO cloning...electro-competent E. coli - excess salt causes arcing during electroporation which would cause the electroporation...

...the gram negative bacteria’s thinner membrane. Procedure The stain is a pretty simple process. First, flood...photos/occbio/7092385559 For more specific procedural instructions, including timing, preparing the... them red or pink. At the end of the staining procedure, gram positive bacteria are stained purple or ...

...a producer cell line like the widely used 293T. During vector production, assembled AAV vector particles...released from the producer cells into the supernatant during the production phase. However, this has not been...at the AAV vectors that are released from cells during production (Maguire et al., 2012). They saw that...

...each of the isotypes. IgM This isotype is produced during a primary immune response because all B cells begin...found in tissues. IgG is the predominant isotype during a secondary immune response, which is when the ...constant domains. This switching helps B cells adapt during the course of an immune response since it also ...

...until it is triggered to enter the lytic cycle.   During both of these life cycles bacterial DNA can be ...in a wild type E. coli (Griffiths et al., 2000). During conjugation, genetic material is transferred from...Guillemin. "Multiple phases of competence occur during the Helicobacter pylori growth cycle." FEMS microbiology...

...the lab. They can be stored at your bench during the procedure and at 4 °C when you’re not running a western...and reprobed multiple times. It doesn’t degrade during long-term storage and has both high binding capacity.... This is because proteins diffuse semiradially during the transfer, making membranes inherently “fuzzier...

...modulate protein activity and have important roles during oxidative stress. To monitor this activity, Pedre...measuring calcium signaling related fluorescence during morphine administration and withdrawal to explore...stimulation of dual virus-expressing CR neurons. (C) Duration of painful responses in the left and right paw...

...mutant cells were treated with WNV at a dose and duration sufficient to kill all control cells and surviving...mutant enables transient suppression of DNA repair during oligonucleotide integration, allowing MAGE in otherwise...polyproteam can be eluted using maltose. This procedure allows for stable and directional protein fusion...need for PCR or additional molecular cloning steps during plasmid assembly. This toolkit reduces the expense... of these genes were investigated for functions during infection of human cells, and one of them called...

..., the vector may undergo genomic rearrangements during replication. To avoid genomic rearrangements, try...result of plasmid integration via recombination during transfection, and does not necessarily indicate...Yezzi, and Barbara R. Young. "Recombination events during integration of transfected DNA into normal human...

...animal’s skull using dental cement (a similar procedure as implanting a guide cannula). A fiber optic ...cable to the ferrule implant must be maintained during the entire protocol, otherwise optical stimulation...that one laser can stimulate only a single animal during the course of an experiment. There are a number...