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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 118 results for: ENDOG

Showing: 41 - 60 of 118 results

  1. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...the target antigen. Some examples could be an endogenous sample of wild type cells, purified protein, ...be sure to include a sample that expresses the endogenous protein, since folding of the recombinant protein...

  2. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...which are cleaved out of the RNA transcript by endogenous processing enzymes (Figure 1). To ensure this...et al. (2024) Improving prime editing with an endogenous small RNA-binding protein. Nature. 2024 Apr 3...

  3. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...typically expressed at much higher levels than endogenous proteins. Data obtained from experiments using...tagged protein may not be translatable to the endogenous system and will need to be verified using alternative...

  4. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...to a histone tail to recruit and stimulate the endogenous methyltransferase. The strategy is generalizable... protein (dCas9 shown here). D3L recruits the endogenous, autoinhibited methyltransferase DNMT3A to the...

  6. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...Tailored mutations are commonly introduced to endogeneous DNA through homology-directed repair (HDR) of...editing requires a template of high homology to the endogenous target, yet to facilitate the repair, the template...

  7. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...reaction. In fact, NLuc is bright enough to detect endogenous tagged genes generated through CRISPR/Cas9 knock-in...knock-in. Finally, NLuc is very inviting for endogenous protein tagging as it is only 19 kDa. An example...

  8. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...albumin. Secondary antibodies can also react with endogenous antibodies naturally present in the sample, for...experimental run (Burry 2011). Autofluorescence Endogenous fluorescence of the sample, or autofluorescence...

  9. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ... Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins...-specific editing of histone modifications at endogenous enhancers. Nature Biotechnology 31:1133–1136 ...

  10. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...that tests enhancer sequences outside of their endogenous chromatin environment and relies on the fact ...because it was difficult to fuse the AID tag to endogenous proteins. As described in their recent Cell Reports...genes of interest to generate mAID fusions to endogenous genes. Cell lines with the appropriate fusions...

  11. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...that do not express your protein of interest endogenously and transfect them with a plasmid encoding your...ultimately planning to use your antibody to detect endogenous levels of the target in other cells or tissues...

  14. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...system described above, except that they use an endogenous nuclease system to cleave the gRNAs. gRNAs are...CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system.” Proceedings of the National...

  15. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ... day of CRISPR, Addgene gave to me: Selective Endogenous eNcapsidation for cellular Delivery (SEND). On...

  17. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...to gather the following types of data: Report endogenous DA release from a single dopaminergic fiber in...

  20. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...separation of detection of a mutant protein vs. endogenous WT levels of the same protein when both are expressed...
Showing: 41 - 60 of 118 results