We narrowed to 77 results for: EXO
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TypeBlog PostPublishedApril 16, 2015, 3:45 p.m.... it may be possible to direct insertion of an exogenous DNA fragment at a Cas9 targeted break (or pair...
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Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog PostPublishedApril 23, 2018, 2 p.m....pathogens. Clift et al. found that addition of exogenous TRIM21 protein along with an antibody targeting... -
What's New in CRISPR - November 2021
TypeBlog PostPublishedNov. 18, 2021, 2:15 p.m....protect the RNA from degradation by cellular exonucleases and broadly improve editing efficiency. Read... -
Human Germline Editing Using CRISPR
TypeBlog PostPublishedAug. 10, 2017, 2:19 p.m.... editing sites in the embryos, as well as whole exome sequencing of embryo derived-ES cells.They also ... -
CRISPRainbow and Genome Visualization
TypeBlog PostPublishedFeb. 28, 2017, 3:30 p.m....labeled protein-coding genes with both intronic and exonic gRNAs. In fact, the method is specific and sensitive... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog PostPublishedMarch 3, 2020, 2:15 p.m....co-infected into the cell. Intein splitting removes any exogenous sequences and regenerates a native peptide bond... -
All in a Twist: dsRNA
TypeBlog PostPublishedFeb. 2, 2023, 2:15 p.m....with toxicity to many organisms. Fig. 1: Exogenous sources of dsRNA Cellular sources of dsRNA... -
Plasmids 101: Golden Gate Cloning
TypeBlog PostPublishedAug. 27, 2015, 2:30 p.m....a few advantages over other cloning methods. Exonuclease-based methods like Gibson assembly require 20... -
Adenoviral Vector Production and Troubleshooting
TypeBlog PostPublishedJune 14, 2018, 11:44 a.m.... made of 3 types of proteins: fiber, penton and hexon proteins. The viral genome DNA is further protected... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog PostPublishedJuly 12, 2021, 1:15 p.m..... Another version of the spike protein, called HexoPro, was developed by Jason McLellan’s lab. This version... -
Making CRISPR Plasmids Using Fragmid
TypeBlog PostPublishedAug. 29, 2024, 1:15 p.m....destination vector via Golden Gate cloning, perform an exonuclease cleanup, transform, plate, and pick. In short... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog PostPublishedAug. 21, 2018, 12:31 p.m....ends. These fragments are then combined with an exonuclease, a polymerase, and a DNA ligase to carry out ... -
xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog PostPublishedMarch 28, 2018, 6:52 p.m....knockout, you might be happy targeting anywhere in an exon. But things get more complicated if you’d like to... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog PostPublishedMay 4, 2017, 2:30 p.m....Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog PostPublishedFeb. 4, 2020, 2:15 p.m....-white screening require X-gal, an expensive exogenously-added substrate, chromoproteins don’t require... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog PostPublishedFeb. 23, 2016, 3:30 p.m....gRNA The gRNA should target near the last coding exon of your gene of interest. For ideal use, this gRNA... -
Viral Vectors 101: Voltage Indicators
TypeBlog PostPublishedJan. 30, 2024, 2:15 p.m....vivo or in vitro and a small molecule is added exogenously to measure a phenotypic response in the form ... -
Of Myc and Men
TypeBlog PostPublishedJan. 19, 2023, 2:15 p.m.... wanted to distinguish between endogenous and exogenous versions of the same target. In the 1980s, Munro... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m....surface proteins (especially fiber’s knob domain, and hexon), as well as other intracellularly-exposed proteins... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog PostPublishedJuly 27, 2023, 1:15 p.m.... membrane, and acquired as an envelope during exocytotic budding, as the viral particle emerges. Retrovirus...