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We narrowed to 237 results for: FAS

Showing: 41 - 60 of 237 results

  1. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...ordering, making it a toss-up as to whether it’s faster to order or try to make it in your lab (but if ... you need a lot of plasmids, it’s almost always faster to order from us!) Ask the PI If you want a plasmid...working at speed or money if budget is a concern. The faster methods, like Gibson assembly, are typically more...

  2. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    Published
    May 15, 2018, 12:43 p.m.
    ...amino acid peptide, the CRY2clust domain enables fast and reversible oligomerization when fused to a protein...termination of light stimulation. CRY2clust enables faster assembly and disassembly dynamics, higher sensitivity...optogenetic system (OptoSTIM1), caused two-fold faster changes in intracellular Ca2+ levels than the original...

  3. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...Kinetics How fast is the process you would like to monitor? Neurons, for example, have unusually fast calcium...three GCaMP types based on their kinetics: GCaMPf (fast) GCaMPm (medium), GCaMPs (sensitive but slower)....

  4. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...have very different disease phenotypes, are two fascinating examples of epigenetics in the form of imprinting...lead to excessive eating and obesity. What is fascinating is that both syndromes can present without any...treat complex or multifactorial diseases, enabling faster diagnosis and treatment and improving patients ...

  5. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...determined by their homology. You might also consider FastCloning, first described by Chaokun Li and colleagues...the prices of the additional enzymes required. FastCloning may be the least expensive of all the options...Addgene!). References 1. Li, Chaokun, et al. "FastCloning: a highly simplified, purification-free, sequence-and...

  6. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...these viruses. The cells are also easy to cultivate, fast-growing, and widely available. The viruses Lentiviruses...like HEK-293T cells, they are easy to cultivate, fast-growing, and widely available. Unlike mammalian ...adenoviruses and the origin of HEK 293 cells. The FASEB Journal, 16(8), 869–871. https://doi.org/10.1096...

  7. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ... 100% negative prediction rate. It is also the fastest test that has been developed so far.  A similar...challenging samples as in Two-Step-SHERLOCK or in a fast, high-throughput manner in one step as One-Pot-SHERLOCK.../2020.04.07.028167 Broughton JP, Deng X, Yu G, Fasching CL, Servellita V, Singh J, Miao X, Streithorst...

  8. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...stimulate at a fast frequency without missing an action potential, then a channel with fast kinetics may...V. S., Deisseroth, K., & Hegemann, P. (2010). Ultrafast optogenetic control. Nature Neuroscience, 13(3...

  9. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...accolades that would launch our careers in science. Fast forward nearly 20 years and I am known by some colleagues...in Jefferson’s case, even a lot about everything. Fast forward a few hundred years and there are currently... will not only get your paper out the door much faster, but getting helpful feedback from other people...

  10. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ... require high sequencing read counts making them fast and viable options for multiplex sequencing in many... for all DSBs, doesn't require high read counts, fast multiplexing Requires delivery of dsODN (potentially... (e.g. dsODN), doesn't require high read counts (fast multiplexing) Requires large cell population, senstive...

  11. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ... of CRISPR/Cas9 technology are coming along at a fast and furious pace. In December, as we've blogged ...it before, but CRISPRs allow research to move so fast." On the very same day the monkey story hit the ...

  12. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ...researchers worldwide. “COVID-19 has spread incredibly fast and without any notion of national boundaries. To...policies and ultimately treatments in a timely fashion.”  With this in mind, Bourque and Tara Moriarty...

  13. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ... (e.g., 100 nm) of DA. In addition, they exhibit fast kinetics (10 ms on and 100 ms off), enabling detection...PMCID: PMC426888. Patriarchi, Tommaso, et al. "Ultrafast neuronal imaging of dopamine dynamics with designed...

  14. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...imaging of voltages. This includes monitoring of fast bursts, as well as slow up/down voltage states in... Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy. Cell...

  15. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...a simple egg is packed with information. I am fascinated with how developmental programs unfold, absorbing...comparative anatomy showed me that some of the most fascinating biology is found in weird and rare organisms ...

  17. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...more about one another. They also provided both breakfast, lunch, and snacks. At the end of the workshop...experiences like workshops at Lab Central. Because of the fast pace of work in the Addgene lab, we don’t often ...

  18. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...easy to manipulate, it’s affordable, and it grows fast to boot. The yeast strains S. cerevisiae and S. ...to culture and easy to work with Grows extremely fast (doubling time of ~ 2 hours) Amenable to genetic...

  19. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...Scientists need fast access to the tools required to study SARS-CoV-2, but with many academic research... the scientific community. Addgene was awarded a Fast Grant to ensure that the scientific community will...
Showing: 41 - 60 of 237 results