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We narrowed to 102 results for: Gene Tools Blue

Showing: 41 - 60 of 102 results

  1. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...component (cyan derivatives) Y66H Blue-shifts the wavelength (blue derivatives) Y145F Increases quantum...from that of the gene of interest, or expressed from the same transcript as the gene of interest, but ...visualize when and where the gene is expressed. Click here to view Addgene’s collection of empty backbones... a wide number of functions, including: tagging genes for elucidating their expression or localization...promoter of interest can be used to effectively monitor gene expression from that promoter in a given cell type...'s got great articles and insight on the newest tools and techniques in fluorescent microscopy. Read...more about optogenetics at OpenOptogenetics and find optogeneic actuators and sensors at Addgene. Cell marking...

  2. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...glutamate), that came in three different colors – blue (SF-Azurite-iGluSnFR), green (SF-iGluSnFR), and ... All Fluorescent Proteins Find AAV Tools References Marvin, Jonathan S., et al. “An optimized fluorescent...zebrafish, and mice. Second Generation iGluSnFR: SF-iGluSnFR In the second generation of iGluSnFR, the Looger...being deposited at Addgene. Other plasmids and pAAVs can be ordered directly from Addgene. The Podgorski ... for Synaptic Imaging However, the first two generations of iGluSnFR had limitations: they were not optimal...extrasynaptic glutamate release. Building on the previous generation of the SF-Venus-iGluSnFR (itself an improvement...IPTG induction to express soluble protein CAG: Generic strong promoter. Formulated as AAV-DJ for neuronal...

  3. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ...fluorescence) are also useful tools to use with intravital imaging. These tools allow one to study the kinetics...technologies such as optogenetics and CRISPR/Cas9 will help immunologists create even better tools and models to...easy and, for many years, researchers lacked good tools to directly follow immune cells in lymphoid organs...when using a standard Ti:Sapphire Laser include: Blue-green fluorescent proteins like EGFP Yellow-orange... light, enabling dual color imaging studies with blue or cyan proteins without changing excitation wavelength...fluorescent proteins. Additional fluorescent protein tools Photoactivable fluorescent proteins (proteins that... for tracking certain cell types. Another useful tool for marking and tracking cells is the the Cre/lox...

  4. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ... combined with blue-light controlled optogenetics, such as channelrhodopsins and tools based on light-...supplying a single gene to cells. Still in bacteria, which do not produce BV, an additional gene coding for heme...of fluorescent proteins, genetically encoded biosensors, and optogenetic tools. Follow her recent research...brightness and monomeric state, are highlighted in blue. aExtinction coefficient. bQuantum yield. cDetermined...-the-art probes, many of which you can find at Addgene. Biliverdin chromophore Near-infrared fluorescent...reported widely used dimeric iRFPs (Table 1). In general, the monomeric state of a probe leads to less interference... Ref BphP-based FPs miRFP670 https://www.addgene.org/79987/ 642 670 87,400 14.0 198 Monomer  155...

  5. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...resistance gene, allowing resistant bacteria to multiply, spreading their resistance genes throughout ...them to multiply and propagate their resistance genes. Our society is hoping to combat antibiotic resistance...bacterial species, or even an antibiotic resistance gene. Importantly, native, non-pathogenic bacteria remain.... targeted beta-lactam and quinolone resistance genes in E. coli using plasmid and phagemid delivery systems...population to the antibiotic, despite this resistance gene being encoded by a high-copy plasmid. In the case...carry CRISPR RNAs (crRNAs) targeting either genomic (blue) or plasmid-based sequences (red). Treating a mixed...CRISPR machinery targeting 1) the given resistance gene and 2) a second lytic phage. Bacteria that receive...

  6. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...used in conjunction with optogenetic activators and inhibitors that respond to blue light. We recently released...our viral service in 2016, we’ve added many new tools to our inventory of ready-to-use viral vectors. ...experiments encoding a fluorescent-tagged molecular tool.   Find pAAV-FLEX-tdTomato, pAAV-GFAP104-mCherry...variant of jGCaMP7 in AAV1 at Addgene! See all calcium sensor AAV at Addgene. GABA sensor AAV GABA is the...pAAV-hSyn-mCherry at Addgene. Viral vectors coming soon! These vectors listed below should be released at Addgene in the...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...the iGABASnFR and its F102G variant in AAV1 at Addgene! Archon1, a voltage sensor AAV Historically, electrical...

  7. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type
    Blog Post
    Published
    June 6, 2019, 12:41 p.m.
    ...developed four acoustic reporter gene (ARG) plasmids to better monitor gene expression in vivo using ultrasound...require custom nucleases for each gene of interest. CRISPR-Cas9 tools have been used in immunology for ...variant Vex. To understand the impact of this gene editing tool on the immune system, the group inserted sgRNAs...Klaus Wanisch  Listen to the optogenetics podcast segment An optogenetic tool that allows light-mediated...plasmids at Addgene. Liu Q et al., Neuron. 2019. PubMed PMID: 30704911. Acoustic reporter genes for noninvasive...Tucker’s labs from UC Denver. At the core of this tool is the endoprotease Botulinum Neurotoxin B (BoNT... light-induced dimerization. Upon application of blue light, iLID undergoes a conformational change allowing...

  8. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...Lavis, 2022). With the wide breadth of fluorescent tools that have been developed throughout the past century...you’re struggling to select the right fluorescent tools, this blog is here to help. Here, we will outline.... When paired with a protein of interest, these tools provide an easier means to identify targets through...include coupling to an antibody, whereby excitation of blue light will indicate where FITC is bound through ...AT-rich regions of double-stranded DNA and exhibits a blue-fluorescent light when excited. While it is technically...for more precise, stable, and versatile imaging tools in biological research. This does make finding the...the dye and the endogenous target, and thus no genetically encoded tag is required. These dyes can also ...

  9. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...researchers. One way of doing that is by using Addgene’s Blue Flame award to identify highly requested GCE...a second plasmid will be needed to introduce the gene of interest containing the chosen codon that will...expansion of GCE led to the creation of many new tools and potential applications. But this focus on development...significant barrier for those wishing to use it as a tool. In short, while the technical skills needed to ...proteins – are not, and many of the existing GCE tools are not very robust. When using GCE, each component... Oregon State University focused on turning GCE tools developed in the literature into robust and universal...limiting. It may be time, then, to look towards genetic code expansion (GCE): techniques in which non-canonical...

  10. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...bright" for the blue laser. To understand why that is, let's look at Figure 2. The blue laser excites at...defined emission maximum. Note, you can also use the tool to display excitation spectra, which I excluded ...Horizon™ BV510 BD Horizon™ V450BD Horizon™V500 Blue (488 nm) BD Horizon™ BB515BD Horizon™ PE-CF594Pe-CyBD...Resources on Addgene.org Addgene's Antibody Guide Ready-to-use antibodies in the Addgene repository Antibody...fluorescent reporter, such as GFP or mCherry, through genetic modification. As your protein of interest will ...sailing, and reading.   More resources on the Addgene blog Antibodies 101: Introduction to Gating in ...

  11. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ... of blue to excite a molecule of GFP. Because red light has approximately half the energy of blue light...are needed to excite GFP compared to only one of blue. Only at the focal point of the objective can a ...techniques has provided biologists with a host of tools and opened doors to many great insights, it can ...PubMed PMID: 26186186.  Additional Resouces on the Addgene Blog Lightsheet Microscopy Choosing Your Fluorescent...Fluorescent Protein for Your Experiments Resources on Addgene.org Browse Our Fluorescent Protein Resources Find...Fluorescent Protein Backbones Find Plasmids for Optogenetics ...

  12. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    Published
    July 6, 2017, 1:59 p.m.
    ...receptor engineering (Ref 5)  as described by Blue Flame Addgene depositor Professor Michel Sadelain from ...addition, it was exciting to see that the use of optogenetic tools is expanding to immunological research. Here...chance to talk with Dr. Friedmann-Morvinski about tools used to analyse the tumor microenvironment (one ...remotely with spatiotemporal precision. This is a great tool to regulate and observe the function of immune cells...Additional Resources on the Addgene Blog Read Additional Cancer Blog Posts Addgene's Cancer Collection Pages...this multi-pronged attack. Inflammation and tumorigenesis The conference opened with a very interesting... between tumor and immune cells in real time. Addgene depositor Professor Max Krummel from UCSF showed...

  13. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...Competition (SPARC) is a fully genetic tool for labeling just a fraction of a genetically-defined subset of cells...the sgRNAiBAR library covers every annotated human gene, it enables genome-wide screening with a high MOI...visualize individual/non-overlapping cells. Until now, tools for doing this were labor-intensive and required...a stop cassette upstream of an effector/reporter gene. In cells where the stop cassette is removed, the...fish, and mice, by replacing the effector with a gene of interest in the cells you are studying. Read ...calcineurin-dependent response elements (CDREs) and drives gene expression. Because calcineurin is required for ...antifungals that target calcineurin. Current calcineurin gene reporters are based on the bacterial βgal assay,...

  14. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...process the variable regions of a parental antibody (blue, rat) are cloned into a backbone expressing the ...they can be easily manipulated into more useful tools. In the case of isotype conversion, the variable...to increase the utility of recombinant antibody tools, the resulting chimera may have different binding...10.1016/s0021-9258(18)99982-5 More resources on the Addgene blog Antibodies 101: Validation Antibodies 101:...

  15. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    Published
    May 24, 2018, 1:20 p.m.
    ...planarian genes, we encourage you to deposit them back with Addgene and expand the number of genes that other...expression of specific gene expression. The 49 deposited plasmids enable students to study gene expression and...planarians. For example, the gene Piwi labels neoblast cells and the gene Prohormone convertase 2 (pc2...Evaluating Gene function by RNA interference (RNAi) RNA interference (RNAi) is a powerful tool for performing...beta-catenin. Other gene targets do not require amputation to be visible. For example, odf2 gene knockdown results...and functions of key regeneration-related genes in four inexpensive and commercially available planarians...types and for viewing how gene expression changes through time or after genetic manipulation. The technique...

  16. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...proteins (FPs) to a yeast gene of interest. These fluorescently tagged genes are expressed under native...sequence and a selectable gene product for isolation of successful transformants. Addgene distributes several... to name a few. Have you used these tools in your own lab? Addgene would love to hear from you, our community...Thorn at UCSF have recently developed a series of blue, green, and red FPs that are codon optimized specifically...be interested in attaching epitope tags to their genes of interest, allowing for easy capture and detection...modifications in S. Pombe, including full and partial gene deletion, overexpression (by promoter substitution...

  17. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...This kind of normalization is a nearly ubiquitous tool, but proceed with caution! Loading controls only...intensity. Researchers use later steps in this chain (blue) to infer or calculate things about earlier ones...S-shaped curve. On the protein abundance axis, both blue “condition 1” points (POI and loading control) are...Optimizing a Western Blot Resources on Addgene.org Addgene's Western Blot Protocol  ...10.1016/j.ab.2013.05.027 Additional Resources on the Addgene Blog Antibodies 101: The Basics of Western Blotting...

  18. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    Published
    July 29, 2014, 6:16 p.m.
    ...attach the gene of interest to either the N-or the C-terminus. Even if a researcher’s gene of interest... to choose from in the green, red, yellow, cyan, blue, and orange spectra. A comprehensive list of cell...cancer that has now prompted him to deposit the tools he and his team created with the research community...researchers as we could, and Addgene is a wonderful vehicle for us to do that.” At Addgene, we are equally excited...is available in an easily searchable format on Addgene’s Michael Davidson Fluorescent Protein Collection...collection with over 300 backbones alone,” said Addgene’s Lianna Swanson, who has been working with members...the scientific community," says Joanne Kamens, Addgene's Executive Director, "and to promote the great ...

  19. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    Published
    Dec. 10, 2015, 3:30 p.m.
    ... We’re adding self service to the repertoire of tools you can use to get support for our resources with...towards the bottom right corner of each page there’s a blue icon displaying the word “Help” next to an encircled...found at help.addgene.org, and the widget is on any page on our regular site, www.addgene.org. We’re excited...comes first. Resources on the Addgene Blog Read other Inside Addgene Posts Learn about New Features ... at Addgene Visit the Help Center Find Plasmids for Your Research Become a Member of the Addgene Community...Addgene’s customer service philosophy Customer service can be a hassle. You finally make it through a... familiar? Well, when it comes to working with Addgene, we certainly hope not! One of our core values ...

  20. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ... Check out this post. Resources on the Addgene Blog 6 Tools & Tips: Online Social Networking for Scientists... The follower network of life (green), physical (blue) and social (orange)  scientists largely cluster...Twitter lists were searched for scientific titles generated from the United States Standard Occupational Classification...scientistswhotweet. And don’t forget to follow us @Addgene.   References Ke, Q., Ahn, Y., & Sugimoto, C....
Showing: 41 - 60 of 102 results