...contains circularly permuted green fluorescent protein (GFP), and nLightR uses a red fluorescent protein, mApple..., Wu, Z., Wu, H., Chen, W., Zhang, P., Zou, J., Hires, S. A., Zhu, J. J., Cui, G., Lin, D., Du, J., & ...

...transcription activation systems, such as LexA/p65 or mCD8/GFP, to allow sparse labeling in a variety of fly cell...transgene (Lin et al., 2018). However, this system requires precise titration of the AAV. So far, tools to...

...using a bacterial expression system such as the pET GFP LIC cloning vector and the pOKD plasmids. The recombinant...acids are essential, which means that the body requires them but cannot produce them. By using yeast integration...

...strain transformed with pBLADE expressing superfolder GFP instead of mCherry under the PBAD promoter. They ...The original AraC system, which uses arabinose, requires many wash steps to remove arabinose making it ...

...or smaller. Some examples include a reporter like GFP, some smaller CRISPR-Cas proteins such as SaCas9,...this awesome experiment you want to do, but it requires some AAV. You’ve never worked with AAV before,...

...also have lentivirus for delivering control shRNAs, GFP, and rtTA from the Tet-on system. The first two can...Virus production can be a frustrating process that requires a large investment of time and resources for labs...

...destination vector contains GFP, enabling you to select cells with high GFP expression. These cells have...editing, and a subsequent curing protocol that requires only 2-3 hours incubation. Kondo Lab multiplexed...

...ones? Many of the available biosensors use a single GFP or CFP/YFP-derived FRET pair. If you need to free...molecular neuroscience. 2014 Nov 18;7:88. Tian L, Hires SA, Looger LL. Imaging neuronal activity with genetically...

...Vectors Read about Reporter Gene like Luciferase and GFP Resources on Addgene.org Find Plasmids for Your...binding sites, commonly used in Drosophila Specific Requires the presence of Gal4 gene to activate promoter...Promoter from T7 bacteriophage Constitutive, but requires T7 RNA polymerase. When used for in vitro transcription... Negligible basal expression when not induced. Requires T7 RNA polymerase, which is also controlled by...Promoter from Sp6 bacteriophage Constitutive, but requires SP6 RNA polymerase. SP6 polymerase has a high ...Promoter from T3 bacteriophage Constitutive, but requires T3 RNA polymerase When used for in vitro transcription...

...check out our article: Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection. Cellular... of hard-to-achieve factors. For example, FRET requires that fusion proteins be in close proximity and...FRET, as the overexpression of fusion proteins it requires can lead to aberrant subcellular localization ... construction Fusing FP fragments to your PIPs requires careful selection of FP fragments and, preferably...information, constructing appropriate controls may requires that you empirically test a number of mutants ...

... enriched relative to controls using Split-BioID-GFP and BioID run on six unrelated proteins. One caveat...of Split-BioID is its speed of labeling. BioID requires 6-24 hours for tetracycline induced expression...

...gene, while adding a GFP-tag (Figure 3). Achieving multiple modifications requires multiple recombination...to transformation (Figure 1). As this approach requires no special reagents or purification kits, it is...

... a superfolder GFP fusion to stabilize the protein in mammalian cells. LwaCas13a-msfGFP can mediate cleavage... tracrRNA and crRNA to cleave target DNA (Cpf1 requires only a crRNA for target recognition and cleavage... PFS. This is in stark contrast to Cas9, which requires that each DNA target have high sequence identity...

...markers were not increased compared to a control AAV-GFP. Not only did the AAV-SaCas9-sgRNA constructs mediate...drawbacks limit the utility of these systems: St1Cas9 requires a very specific PAM sequence that limits the number...

...aptamers able to bind to a small molecule derivative of GFP called 3,5-difluoro-4-hydroxybenzylidene imidazolinone...instability at 37°C (Ouellet et al., 2016) and requires a tRNA scaffold to improve its stability in vivo...

...fluorescent protein that could be co-imaged with GFP using the same wavelength for excitation. The result...for how sensitive you can make a reaction. NLuc requires 100-fold less protein to produce the same amount...detecting traditional luciferases’ dim light emissions requires long acquisition times that may be impractical...

...are generated by inserting a circularly-permuted GFP into an intracellular loop of various G-protein-coupled...variations and heat transfer at the subcellular level requires a probe with fast kinetics and high signal/noise...

..., Hess et al. successfully evolved wild type GFP to EGFP using CRISPR-X and subsequent FACS sorting. They...with unique tissue-specific promoters expressing GFP, tetracycline response elements, and shRNAs many ... of DsRed connected to a pH-sensitive variant of GFP (SEP) by a 9 amino-acid linker (see Figure 1). The...for more selective targeting of fusion proteins, requires less biotin, and shows enhanced labeling of proximate...

...labeling. Size is also a factor. Bulkier tags, such as GFP, may interfere with protein expression or function...another factor to consider. If your application requires longer periods of exposure, you’ll want a more...

...cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles are removed from cells by dilution...promoters for transcriptional regulation, so it requires a different approach for regulating transgene ...Generating iPSCs with plasmid-based expression requires serial transfection of 1 or 2 plasmids that express... the gold standard instead. Teratoma formation requires injecting iPSCs into immunodeficient mice and ...suited for these situations. The second scenario requires higher levels of safety and as little chance of...