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Showing: 41 - 60 of 169 results
  1. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...available in the Sybody Generation Toolbox plasmid kit: SB_concave, SB_loop, SB_convex. These scaffolds ... This selection step involves using a commercial kit for in-vitro translation of the mRNA libraries. Each...plasmids are available in the Sybody Generation Toolbox kit. Find the Seeger lab's SARS-CoV-2 sybodies! References...
  2. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ..., plasmid kits are avaliable for less than than it would cost to buy each plasmid in the kit individually...This describes how the plasmid or other product (kit, pooled library, etc) will be sent to you. Most of...
  3. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ... tools that you need. The Golden GATEway cloning kit simplifies the cloning process for complex DNA constructs...biological systems” as they predict. The pOSIP plasmid kit can be found at Addgene, so what are you waiting ...constructs, while the Patron Lab MoClo Plant Parts kit contains modules specific for plant transformation... For further reading about Nicola Patron's MoClo kit and her plant synbio research, read the Addgene interview...annealing (SSA) assay. This new Platinum Gate TALEN Kit utilizes a 4-module assembly system, which reduces...additional information on using the Platinum Gate TALEN Kit, please see the Yamamoto lab’s protocol for TALEN...specifically to improve plant transgenesis. TheGreenGate kit available at Addgene includes six individual types...
  4. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...and responsive voltage indicator ONE-GO biosensors kit Antibodies for neurodegeneration research   Optimized....1016/j.neuron.2024.08.019.  ONE-GO biosensors kit By Mike Lacy G protein-coupled receptors (GPCRs) ... of selectivity. With 10 plasmids available as a kit, plus 21 optional accessory plasmids, the collection...
  5. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...topoisomerase (2).  These days commercially available TOPO kits provide vectors or cloning arms with overhanging...covalently linked to topoisomerase. Vectors in these kits also often have the topoisomerase site inserted ...
  6. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...of the PCR reaction using a kit, such as the QIAquick PCR Purification Kit. The PCR product is now ready...
  7. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ... the entrepreneurial bug and has set up an ELISA kit company on whose website you can find some great ...great information on ELISA troubleshooting, ELISA kits and cell death assays. You can find Seán on twitter...
  8. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...Subtype Promoter Example Activator Addgene Plasmid/Kit Alcohol-regulated AlcA promoter AlcR pGGA008...AlcA) and pGGC011 (AlcR) from red flame GreenGate kit Steroid-regulated LexA promoter XVE (synthetic...
  9. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...done, through a variety of methods or commercial kits.  However, antibodies conjugated to horseradish ...that can be activated to emit light with a simple kit, and is readily available on any number of secondary...
  10. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ... can be done in-lab using commercially available kits or ordered as synthesis modifications when purchasing...choose from and you can opt to do-it-yourself with a kit or purchase it commercially synthesized with a label...
  11. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...Sandwich ELISA Most commercially available ELISA kits are for a sandwich ELISA. This technique works by...interested in detecting in your sample. When an ELISA kit is ordered, the wells are typically already coated...
  12. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...unknown GPCRs. You can find the entire PRESTO-TANGO kit at Addgene! TRUPATH: Bryan Roth’s lab also assembled...ligand-bound GPCRs of interest. The TRUPATH plasmid kit consists of 20 plasmids encoding 14 alpha, 2 beta...
  13. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...little more colorful? CRISPRainbow is available as a kit from Addgene.   References 1. Chen, Baohui, et al...Fluorescent Protein Plasmids Find Fluorescent Protein Kits Find Empty Backbones with Fluorescent Protein Tags...
  14. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...looking to conjugate antibodies yourself, there are kits available to make the process quick and easy. Before...antibody’s buffer is compatible with your conjugation kit, since many common antibody buffers and buffer additives...
  15. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...there are several commercially available p24 ELISA kits that can quantify p24 levels in a matter of hours...day, but tends to be less expensive than an ELISA kit. In this approach, viral RNA is first converted to...
  16. Twenty Years of Sharing Science!

    Type
    Blog Post
    Published
    Jan. 8, 2024, 3:25 p.m.
    ...repository for DNA-based materials, including our popular kits and libraries. This DNA-based materials repository...
  17. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ... you prepaid shipping materials (i.e., a deposit kit) with instructions on how to send liquid DNA or bacterial...pages for your review. We’ll also mail you a deposit kit with instructions on how to prepare and ship the ...
  18. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ... colonies, sub-culture, and prep with a DNA prep kit or your lab's favorite in-house protocol. DNA yields...lead to degradation of your prep. Many plasmid prep kits recommend performing an extra wash step to remove...
Showing: 41 - 60 of 169 results