We narrowed to 619 results for: LIS;

...doi.org/10.1104/pp.15.00636 Ma X, Zhang X, Liu H, Li Z (2020) Highly efficient DNA-free plant genome editing... . https://doi.org/10.1073/pnas.1818290116 Zhu H, Li C, Gao C (2020) Applications of CRISPR–Cas in agriculture...Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the...

...prep pAAV-hSyn-GRAB-gDA3m AAV1 Biosensors Yulong Li New viral prep pGP-AAV-GFAP-iGABASnFR2-WPRE AAV1...

..., Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park J, Blackburn EH, Weissman JS, Qi LS, Huang...that there’s a new CRISPR advance or technique published every week! One of the newest applications is ...fluorescent distances correlating with the previously established physical map. In comparing pairs of targets ~...

...Lentiviral CRISPR-Guide RNA Library. Koike-Yusa H, Li Y, Tan E-P, Del Castillo Velasco-Herrera M, Yusa ...

...gene is synthetic or natural? The answer to this lies in the codon usage biases of different organisms... in synthetic biology and DNA synthesis. They published their results in a paper in Nature Communications... Nielsen & Christopher A. Voigt also recently published a tool (trained on Addgene plasmid datasets) to...

...to offer AAV for the newest GRAB sensors from the Li Lab. These GPCR based sensors provide the spatiotemporal...

...Addgene’s traditional excellence through whatever lies ahead this year. We can see signs of the storm passing...

...Hires, S. A., Zhu, J. J., Cui, G., Lin, D., Du, J., & Li, Y. (2019). A Genetically Encoded Fluorescent Sensor...and resources References Feng, J., Zhang, C., Lischinsky, J. E., Jing, M., Zhou, J., Wang, H., Zhang, ...

...serotypes pAAV-hSyn-GRAB_OTmut AAV1 Biosensor Yulong Li New viral prep pAAV-CAG-NIR-GECO2G AAV1, AAV2,...

... with the FP mNeptune684 (originally described by Li ZY, et al. PLoS One, 2016) and tested a series of...a recent plasmid deposit please sign up here.   Listen this episode of Hot Plasmids! Light controls the... dynamics Article contributed by Klaus Wanisch  Listen to the optogenetics podcast segment LOVTRAP is...localization Article contributed by Tyler Ford  Listen to the riboglow podcast segment Researchers from... story Article contributed by Caroline LaManna  Listen to the mCarmine podcast segment Scientists are ...activity Article contributed by Angela Kaczmarczyk  Listen to the base editing podcast segment The increasing...

...Second generation GRAB dopamine sensors from Yulong Li’s lab are now available including: pAAV-hsyn-GRAB_DA2h...

...characterized by the Zhang (Strecker et al., 2019) and Li (Teng et al., 2018) Labs also shows promise for in...In January 2016 we first published a blog post titled: Which Cas9 Do I Choose for My CRISPR Experiment...

.../doi.org/10.1128/jvi.02012-06 Ou X, Liu Y, Lei X, Li P, Mi D, Ren L, Guo L, Guo R, Chen T, Hu J, Xiang...

... 5: . https://doi.org/10.1038/ncomms5286 Yan M-Y, Li S-S, Ding X-Y, Guo X-P, Jin Q, Sun Y-C (2020) A CRISPR-Assisted...

... E. coli strains including the popular BL21(DE3) (Li et al., 2021). This pEcCas/pEcgRNA system works similar...–1290 . https://doi.org/10.1021/acssynbio.7b00399 Li Q, Sun B, Chen J, Zhang Y, Jiang Y, Yang S (2021)...Originally published Mar 3, 2016 and last updated Apr 13, 2021 by Will Arnold. Although CRISPR systems...bacterial CRISPR engineering One common way of accomplishing bacterial genome engineering is done with recombineering...the above CRISPRi section, the Marraffini Lab published a robust tool for CRISPRi and CRISPRa using RNAP-Omega-dCas9...systems is base editing. This process is generally accomplished by fusing an enzyme capable of converting one...

...nar/gkw930 Yu Y, Wu X, Guan N, Shao J, Li H, Chen Y, Ping Y, Li D, Ye H (2020) Engineering a far-red light–activated... LaManna, published Mar 8, 2016. The updated and expanded version by Nyla Naim was published Sept 3, 2020...dCas9 Initial photoactivatable CRISPR systems published in early 2015 focused on using light to control... and repeatable. Since these initial studies published in 2015, more split systems have been developed...Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems.  Listen to the anti-CRISPR podcast segment Simply expressing...

... Zhu, Z., Liu, G., Kou, L., Wang, Y., Qiu, J.-L., Li, J., & Gao, C. (2021). High-efficiency prime editing...., Levesque, S., Jing, Y., Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt, R., Solley, S. ...CRISPR editing world. The approach, originally published in Nature, is a “search-and-replace” genome editing...

...References 1. Zhou, Xin X., Linlin Z. Fan, Pengpeng Li, Kang Shen, and Michael Z. Lin. "Optical control ...

...gene that kills the cells expressing it? The answer lies in special strains of E. coli that tolerate the ...take up your insert. But exactly how does ccdB accomplish this? Let’s start with a brief history of the...

...experiment! References Becher, Burkhard, Ari Waisman, and Li-Fan Lu. "Conditional gene-targeting in mice: problems...