We narrowed to 58 results for: LucY
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TypeBlog PostPublishedMarch 11, 2025, 1:15 p.m.... a YFP-tagged G protein and a NanoLuc® (Nluc) Luciferase-fused detector that specifically binds to active...
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Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog PostPublishedOct. 12, 2017, 2:26 p.m....donor plasmids for protein tagging, including luciferase (NanoLuc), fluorescent proteins (TagGFP2, TagBFP... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog PostPublishedApril 19, 2016, 2:30 p.m....Protein Resources Find Other Plasmids that Glow (Luciferase Plasmids) Find Optogenetics Resources ... -
Plasmids 101: Multicistronic Vectors
TypeBlog PostPublishedSept. 9, 2014, 8:20 p.m....Retroviral pEF1a-IRES-Neo IRES Mammalian MSCV-IRES-Luciferase IRES Retroviral pWPI IRES Lentiviral AmCyan-P2A-mCherry... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog PostPublishedJune 12, 2014, 3:30 p.m....disease. They also devise biosensors designed to elucidate the molecular interactions that go on between ... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog PostPublishedDec. 2, 2014, 7:23 p.m....as transcription and epigenetic regulation. To elucidate molecular mechanisms of regulation of genome functions... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog PostPublishedAug. 13, 2019, 12:58 p.m....types. Sequencing-based lineage tracing can help elucidate the gene networks that transform zygotes into ... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog PostPublishedFeb. 17, 2022, 2 p.m....RNA editing tools. Fig. 1 Schematic of luciferase RNA knockdown assays using Cas7-11 Use ... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog PostPublishedNov. 21, 2024, 10:47 p.m.... backbones, lentiviral packaging systems, and luciferase expression. On individual material pages, we ... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog PostPublishedNov. 1, 2018, 12:35 p.m....Cara E., and James B. Kaper. "Use of stabilized luciferase-expressing plasmids to examine in vivo-induced... -
Plasmids 101: Control Plasmids
TypeBlog PostPublishedApril 9, 2015, 3:29 p.m....constitutively expresses a reporter protein (e.g., GFP or luciferase) that is either co-transfected with the test ... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog PostPublishedApril 3, 2014, 8:05 p.m....Mammalian Vectors Read about Reporter Gene like Luciferase and GFP Resources on Addgene.org Find Plasmids... -
An Integrin Antibody Toolkit from IPI
TypeBlog PostPublishedMarch 28, 2024, 1:15 p.m....cells to spread into symmetrical circles. While elucidating the role of tension, however, the researchers... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog PostPublishedMay 10, 2016, 2:30 p.m....binding sites increased activation of HIV promoter-luciferase constructs. In multiple HIV-1 latent T cell lines... -
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog PostPublishedSept. 29, 2016, 2:30 p.m.... enzyme with a short recognition sequence (like MluCI) that will cleave DNA frequently enough to result... -
Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog PostPublishedJuly 28, 2016, 2:30 p.m....PBAD), and the A118 gene downstream of the diacetylphloroglucinol (DAPG)-inducible promoter (PPhlF). Output... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog PostPublishedMarch 6, 2018, 1:49 p.m....PubMed Central PMCID: PMC1978145. 27. Greenough, Lucia, et al. "Adapting capillary gel electrophoresis ... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog PostPublishedSept. 28, 2020, noon...stranded breaks (DSB) are lethal. Addgene depositor Luciano Marraffini’s lab took advantage of this lethality...