We narrowed to 87 results for: PEP

...short genetically encoded tetracysteine (Cys4) peptide tag could be used to label proteins in cells (Figure...covalent attachment of a fluorophore ligand to a peptide tag (Figure 1c). Click chemistry (e.g., transcyclooctene–tetrazine...

...neuronal circuits. Epitope tags are short antigenic peptide sequences, attached to a protein of interest (POI...simian virus 5-derived epitope (V5), the synthetic peptide FLAG, the synthetic streptavidin-binding strep-...

...Addgene’s catalog is a new class of sensors for neuropeptides and neurotransmitters. Motivated to develop ...GPCRs recognizing various neurotransmitters, neuropeptides and other ligands - almost A to Z! - from ATP...

...recombinant antibody. They are ~25 kDa single polypeptides that contain the variable light chain (VL) and... two chains are connected by a flexible linker peptide that is usually 15-20 amino acids long and made...

...extension and translation of the pSer-incorporated polypeptide. These three components were shown to effectively...proportional to a single phosphate moiety on the peptide of interest.  After confirming pSer incorporation...

...Laminin receptor (LR)3 AAV receptor (AAVR)2 Carboxypeptidase D (CPD), also called AAVR25   AAV9 Terminal...receptor (LR)3 Putative integrin3 AAV11 Carboxypeptidase D (CPD), also called AAVR25   1Wu, Miller...

.... Mark Howarth’s lab recently engineered a new peptide/protein pair, SnoopTag/SnoopCatcher, that can be...link desired proteins together via a spontaneous isopeptide bond. When used in combination with their previously...

...like high resolution Ras sensors and crosslinked peptides proposed as a scaffold for a new generation of...

...This is where affinity tags come in – they are peptides which do not naturally exist in most expression...

...type tropism. Introducing changes and/or short peptides sequences into these variable loops is one method...

...transduction strategy is to fuse a cell penetrating peptide (CPP) to a protein of interest thereby giving it...

...the pMinDisplay backbone is fused to a specific peptide segment (PDGFR TMD) that anchors the sensor to ...

... translated, the SunTag sites on the nascent polypeptide recruit scFV-GFP, generating green puncta that...

...of K. phaffii lacks certain terminal mannose glycopeptides (which most other yeast possess) that are problematic...

...protein. Animals are first injected with a small peptide that represents a short, unique amino acid sequence...

... the protein of interest by a 2A self-cleaving peptide (Goedhart et al, 2010). An explanation of the assay...

...want to generate antibodies against, or a unique peptide sequence derived from this protein. At the same...

...are secreted from cells due to their IgGκ signal peptide sequence, making them easy to harvest from cell...

...protein synthesis: incorporation of puromycin into peptide chains." Proceedings of the National Academy of...

...any exogenous sequences and regenerates a native peptide bond at the split site. Figure 1: The base...