We narrowed to 185 results for: PID

...students to explore how engineering fields rely on rapid prototyping to develop and test hypotheses. Students...Students then use cellular extracts as a means to rapidly prototype DNA circuits and logic gates. This BioBuilder-Caltech...

...fluorescent protein (RFP), named GEMCherry2, that can rapidly and efficiently evaluate Cas9 cutting sites and... GEmCherry2 reporter systems provide efficient, rapid, and real-time read-outs of Cas9/gRNA efficiency...

...synthetase introduced to the bacteria using plasmids. Rapid expansion occurred, with hundreds of new ncAAs and... any number of experiments with. The catch The rapid expansion of GCE led to the creation of many new...

...chromosomes is partially or completely missing in women. Bicuspid aortic valve (an aortic valve that has two leaflets...study is to understand why women with TS develop bicuspid aortic valve at a rate that is 50-100 times higher...

...what if we had a simple tool that allowed us to rapidly decode the complexity? The CombiGEM-CRISPR technology...for genome editing in large-scale has led to the rapid generation of genome-wide gRNA libraries that knock...

...cell line containing an activating mutation in epidermal growth factor receptor (EGFR) with the ClonTracer...distinct evolutionary paths to become resistant to epidermal growth factor receptor inhibition." Nature medicine...

...References 1. Luo, Jinyong, et al. "A protocol for rapid generation of recombinant adenoviruses using the... the AdEasy System. 3. Jager, Lorenz, et al. "A rapid protocol for construction and production of high-capacity...

...orange-red fluorogen A split fluorescent reporter with rapid and reversible complementation. Tebo et al. Nat ... for chromophore maturation, splitFAST displays rapid and reversible complementation. TagBiFC (split HaloTag...

...for future CRISPR therapies CRISPR technology is rapidly evolving, and many of the challenges with therapies...delivery technologies are suited for. For example, lipid nanoparticle delivery tends to be trafficked to ...

...degron (AID) tag to generate proteins which can be rapidly and reversibly degraded after the addition of auxin...Central PMCID: PMC4974478. Natsume, Toyoaki, et al. “Rapid Protein Depletion in Human Cells by Auxin-Inducible...

... been removed. The degradation and reversal are rapid compared to other systems because the system modulates...Cleveland DW. Inducible, reversible system for the rapid and complete degradation of proteins in mammalian...

...5’-phosphorylated ssDNA guides, which might be rapidly degraded by the cellular machinery, leaving a small...system, but the genome-editing field is advancing rapidly and it wouldn’t be a surprise if someone came up...

...gRNA complex and delivered to cells using cationic lipids. Transient expression of CRISPR components, expression...delivery methods below: Chemical transfection   Lipid mediated Cationic polymers Calcium phosphate Cells...

...References 1. Jones, Douglas H., and Bruce H. Howard. "A rapid method for recombination and site-specific mutagenesis..., Peter, Monica Winkler, and Wilfried Bautsch. "Rapid cloning by homologous recombination in vivo." Nucleic...

... References Kim, Y., & Morrison, S.L. (2009). A Rapid and Economic In-House DNA Purification Method Using...Peng, F., Huang, L., Zhao, S., & Deng, W. (2018). Rapid Regeneration and Reuse of Silica Columns from PCR...

...lateral flow strips are easy to transport and work rapidly, providing reliable results in as little as an ...within one hour. DETECTR thus has the ability to rapidly detect nucleic acids with high selectivity and ...

...various pieces were connected, as this enabled us to rapidly iterate and optimize our pipeline. After reviewing...plasmid sequences on a 96-well plate basis, but for rapid testing and refinements, we specified a select small...

...interest) and mScarlet-I3 for scenarios requiring rapid maturation and prolonged imaging with minimal background...2024). A dual-plasmid CRISPR/Cas9-based method for rapid and efficient genetic disruption in Mycobacterium...

...other biomolecules of interest e.g. DNA, RNA, and lipids. Still, these molecules can be indirectly visualized...1. Zacharias, David A., et al. "Partitioning of lipid-modified monomeric GFPs into membrane microdomains...

...fusion does not (figure 2). In the mVenus-APT1, a lipidation motif, which is needed for Golgi localization...PMC2853173. Sheridan, Douglas L., et al. "A new way to rapidly create functional, fluorescent fusion proteins:...