...mix derived from cells or tissues. Westerns are useful not only to detect the presence or absence of a...should be repeated.  Finally, while westerns are a useful way to gauge protein abundance they provide qualitative...before and after treatment.  Westerns are also a useful readout for a number of cellular assays. They can...

...classes of phage proteins to study. These are highly useful for the biotech industry, which is in the process... gene function. In this post, I will describe a useful and cost-effective method that our lab (the Szymanski... proteases knocked out making them particularly useful for protein production), then dilute and plate ...

...transposon vectors with us to help us expand this useful resource. While Addgene cannot distribute plasmids... 256 base-pairs of DNA sequence, making it very useful for genetic engineering approaches. Most importantly...expression levels of your gene may be particularly useful when the gene being expressed has threshold effects...

...them into appropriate containers. The machine is useful for distinguishing cell populations that have been...green fluorescence to red fluorescence) are also useful tools to use with intravital imaging. These tools...available for tracking certain cell types. Another useful tool for marking and tracking cells is the the ...

...of scatter is captured by detectors and provides useful information about the cell. Light that continues...of secondary antibodies. It’s also particularly useful for intracellular staining where binding of large...flow cytometry for single cell analysis and some useful background information for setting up antibody ...

...may find “Chapter 1: What is an Antibody” quite useful. If you’re looking to design your first flow cytometry...find the latest addition to our eBook collection useful. And, as always, don’t hesitate to reach out to...

...reach everyone. Therefore, to make our library more useful to our community, we’re asking you for plasmid ...expand the repository in ways that will be most useful to you, the members of our community.  ...

...regularly as people send me suggestions. The most useful parts of the Guide are probably the papers and ...find. I hope that many people will find this guide useful as they start their synthetic biology journey. ...

...constantly evolving, but sometimes a technology is so useful it forever enhances biological research and discovery...Skimming the list, one gets a sense of the broad usefulness of pMD2.G and VSV-G. This plasmid has been used...

...other than fluorescent proteins can be incredibly useful in FRET experiments. Professor Fessenden's example...on any of our posts if you have any questions or useful insights. If you're interested in blogging for ...

...Luminescent molecules are very useful tools because we can easily detect and measure the light they emit...on GFP. This great honor serves to highlight how useful fluorescent proteins like GFP really are. In this...

...leader,” develop a technical “niche,” develop a useful new assay, master a new technique and be able to... are passionate about it.   Maintain a content-useful website, write a blog Pursue speaking invitations...

...year running, and you can learn more about this useful, DREADD-containing AAV here. But the top requested... specifically in neurons. AAV Delivered Cre - A useful tool for neuroscientists AAVs are widely used by...

...of deletions by paired DSBs may be particularly useful. The first is the study of non-coding regulatory...just a single intact copy at a given locus may be useful to study haploinsufficiency. Subsequent modifications...

...vectors with a larger cloning capacity would be very useful: enter pSIM24! To build a better binary vector,... also be used with biolistic methods, making it useful for engineering plants that are not susceptible...

... Leeson. Here at Addgene, we like to help share useful new resources with the scientific community – particularly...    Fig. 1: HeLa cells. Image courtesy of Josef Reischig.  Contributing to Cellosaurus Generated...

...developments (and, of course, find plasmids that will be useful to you.) With these goals in mind, we set out ...web resources and plasmid pages to make them more useful and easy to navigate. We also updated some our ...

...researcher looking for molecular biology tools, a very useful trait. Virus structure While all viruses have a... the viral vector, increasing both safety and usefulness of the tool. In fact, most gutted genomes have...

...thankfully many other applications that they are useful for! What the HEK is it good for?   As one of ...of research where HEK cells may be particularly useful due to their intrinsic properties. HEK-293 cells...

...peak fluorescence and resting fluorescence. It is useful for activity imaging of large populations of densely...peak fluorescence and resting fluorescence.  It is useful for activity imaging of large populations of densely-labeled...