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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 342 results for: SEF;

Showing: 41 - 60 of 342 results

  1. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...transposon vectors with us to help us expand this useful resource. While Addgene cannot distribute plasmids... 256 base-pairs of DNA sequence, making it very useful for genetic engineering approaches. Most importantly...expression levels of your gene may be particularly useful when the gene being expressed has threshold effects...

  2. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...mix derived from cells or tissues. Westerns are useful not only to detect the presence or absence of a...should be repeated.  Finally, while westerns are a useful way to gauge protein abundance they provide qualitative...before and after treatment.  Westerns are also a useful readout for a number of cellular assays. They can...

  3. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ...Tervo et al., 2016). This functionality makes it useful for mapping neural connections. Although derived...can also cross the blood-brain barrier, making it useful for applications targeting the CNS (Issa et al.... tissue tropism, different AAV serotypes can be useful to evade neutralizing antibodies developed from...

  4. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ...them into appropriate containers. The machine is useful for distinguishing cell populations that have been...green fluorescence to red fluorescence) are also useful tools to use with intravital imaging. These tools...available for tracking certain cell types. Another useful tool for marking and tracking cells is the the ...

  5. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...of scatter is captured by detectors and provides useful information about the cell. Light that continues...of secondary antibodies. It’s also particularly useful for intracellular staining where binding of large...flow cytometry for single cell analysis and some useful background information for setting up antibody ...

  6. Introducing the Addgene Antibodies 101 eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2024, 1:15 p.m.
    ...may find “Chapter 1: What is an Antibody” quite useful. If you’re looking to design your first flow cytometry...find the latest addition to our eBook collection useful. And, as always, don’t hesitate to reach out to...

  7. Suggest A Plasmid You'd Like to Find at Addgene

    Type
    Blog Post
    Published
    March 16, 2016, 5:30 p.m.
    ...reach everyone. Therefore, to make our library more useful to our community, we’re asking you for plasmid ...expand the repository in ways that will be most useful to you, the members of our community.  ...

  8. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...regularly as people send me suggestions. The most useful parts of the Guide are probably the papers and ...find. I hope that many people will find this guide useful as they start their synthetic biology journey. ...

  9. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ...constantly evolving, but sometimes a technology is so useful it forever enhances biological research and discovery...Skimming the list, one gets a sense of the broad usefulness of pMD2.G and VSV-G. This plasmid has been used...

  10. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ...other than fluorescent proteins can be incredibly useful in FRET experiments. Professor Fessenden's example...on any of our posts if you have any questions or useful insights. If you're interested in blogging for ...

  11. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 7, 2017, 1:58 p.m.
    ...Luminescent molecules are very useful tools because we can easily detect and measure the light they emit...on GFP. This great honor serves to highlight how useful fluorescent proteins like GFP really are. In this...

  12. Developing Transferable Skills During Science Training

    Type
    Blog Post
    Published
    June 3, 2014, 2:14 p.m.
    ...leader,” develop a technical “niche,” develop a useful new assay, master a new technique and be able to... are passionate about it.   Maintain a content-useful website, write a blog Pursue speaking invitations...

  13. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    Published
    Jan. 17, 2018, 2:57 p.m.
    ...year running, and you can learn more about this useful, DREADD-containing AAV here. But the top requested... specifically in neurons. AAV Delivered Cre - A useful tool for neuroscientists AAVs are widely used by...

  15. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ...vectors with a larger cloning capacity would be very useful: enter pSIM24! To build a better binary vector,... also be used with biolistic methods, making it useful for engineering plants that are not susceptible...

  16. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...developments (and, of course, find plasmids that will be useful to you.) With these goals in mind, we set out ...web resources and plasmid pages to make them more useful and easy to navigate. We also updated some our ...

  17. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ... Leeson. Here at Addgene, we like to help share useful new resources with the scientific community – particularly...    Fig. 1: HeLa cells. Image courtesy of Josef Reischig.  Contributing to Cellosaurus Generated...

  18. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ...scientists working at home, and you will still find this useful. Now that we’ve been doing this for almost 6 months...Giving a great video presentation is going to be a useful skill for the foreseeable future and is a great...

  19. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...build the collection, we hope that you will find useful antibodies for your research. Please consider sharing...This data will help the collection to become more useful to everyone over time. Through our incredible ...

  20. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...descendants of the heterozygous plants, therefore, are useful for research, and they are identified by a phenotype...able to produce seeds. However, they will not be useful to study because they will no longer show the effects...
Showing: 41 - 60 of 342 results