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We narrowed to 351 results for: SUA

Showing: 41 - 60 of 351 results

  1. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...replication, selection, and expression. Replication It’s usually not enough to get your vector into your target ...both target species. Fun fact! Plant vectors are usually designed to shuttle between E. coli and Agrobacterium...a reporter, such as a fluorescent protein, to visualize vector uptake. These reporters might be used as... as markers for automated cell sorting or to visually select cells in a microscopy image for individual...

  2. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...and is therefore inefficient in mammalian cells (usually grown at 37 °C). Smart molecular evolution led ...Logie and Stewart 1995; Metzger et al. 1995). Visualizing single neurons in the living brain   ...structure of a neuron and its synapse should be visualized at the same time from two different plasmids ...together with inducible Cre recombinase (CreERT2), visualizes individual neurons and their synapses in a conditional...

  3. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...recognition and “productivity”, so this is unlikely. Usually, the originating scientist and colleagues are privileged... amounts of data. When published, this data is usually condensed into a statistically evaluated set of...standards of data recording are highly variable and usually left to individual researchers. Yet accurate and...publications presenting a polished, standard and usually limited view of data. In addition to enhancing ...

  4. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...(ii) no need to add a co-factor, (iii) easy visualization with standard filter sets on a fluorescence ...lipids. Still, these molecules can be indirectly visualized by using protein domains that specifically bind...the sample. Although bioluminescent probes are usually dim relative to fluorescent probes, recent engineering...Adjobo-Hermans, Merel JW, et al. "Real-time visualization of heterotrimeric G protein Gq activation in...

  5. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...the fine points of visual aids. Here’s a great slideshare with more details on visuals in science presentations...talk (not a lab meeting) in the previous year. Usually fewer than 10% have done so. How can you expect...Finally, don’t pass up opportunities to show cool visuals, but don’t overdo the effects. It may be a bit ...

  6. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...sequence on either side of the desired mutation (usually 1-3 mismatched bases) is sufficient for your primers...sequences, but if present, a minor extension can usually ensure that the 3’-base(s) do not form secondary...facilitated by the addition of DMSO to the PCR reaction (usually around 3% final concentration). The DMSO reduces...plasmid. Note that any residual parental plasmid (usually from incomplete DpnI digest) can also form colonies...

  7. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    Published
    Aug. 5, 2025, 1:15 p.m.
    ... student at Moffitt Cancer Center. Engineering usually calls to mind building things, lots of math, and...sequences and structures to affect their function. Usually, the goal is to enhance their efficacy, safety,...complete each cycle, your best-performing mutant is usually the starting point for the next cycle. ...of different primers. While error-prone PCR is usually employed to mutate whole antibody sequences, degenerate...

  8. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    Published
    June 26, 2014, 2:23 p.m.
    ...proper ladder. These controls help you properly visualize your results.The most important of those is to...comes to DNA sequencing the chromatogram is your visual control. And, like all controls, missing out is...bases of a DNA sequencing read The peaks here are usually unresolved and small, so I suggest designing your...

  9. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...detect a given molecule. The sensor component is usually a protein that undergoes a conformational change...Localization within cells Fluorescence microscopy Visualize components of individual cells Separation/identification...Aptamer Fluorophores Use CRISPRainbow for Genome Visualization Resources on Addgene.org Find Biosensor Plasmids...

  10. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...different secondaries for your antibody pair and visualize both targets in parallel (Fig. 2). For example...secondary to detect Protein B and successfully visualize both proteins.     Figure 2: Two primary...fluorescent labels and both targets can be successfully visualized (bottom panel).    Can any antibody be converted...

  11. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...Interactive Fluorescent Protein Property Visualization allows for visual comparison of FPs and how they relate...collaborated with him on a web-based interactive visualization of FP attributes (which turned into www.fpvis.org...

  12. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ... local exercise spot or find something new. We usually have yoga class twice a week at Addgene HQ, but...presentations. Learn a new skill for analyzing and visualizing your data, such as R or Python. Write a manuscript...idea is not necessarily to chat, but to mimic your usual work environment.  Be kind to yourself. This is ...

  13. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...Inefficient Ploidy Usually diploid, but spontaneously changes Usually haploid Usually haploid Pathology...

  14. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...unmeasurable skills and productivity coming from that casual discussion on the way to the office or in the kitchen... flexible work schedule the more we realize how casual chatter is very much missed in the virtual world...widely used and could bring back a taste of that casual kitchen chatter, ultimately sparking new ideas....

  15. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    Published
    Aug. 28, 2014, 3:34 p.m.
    ... once allow you to more easily and accurately visualize the full size of your construct. Consider buffer...pouring it. EtBr binds to the DNA and allows you to visualize the DNA under ultraviolet (UV) light. Don’t forget... settles in the gel well and the dyes provide a visual reference point so you can easily assess how far...

  16. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...than nitrocellulose membranes. Pro tip! You can visualize proteins on a PVDF membrane without staining; ...protein (HRP) and alkaline phosphates. HRPs are usually preferred because of their higher specific activity...chemiluminescence enzymes, making it easier to accurately visualize proteins of wildly different amounts in the same...background Direct detection method Less sensitive; usually more expensive Nitrocellulose or low-fluorescence...

  17. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    Published
    July 6, 2017, 1:59 p.m.
    ...is a dangerous state for the body to be in and usually self-limiting effects kick in trying to resolve...and Defense With increasingly sophisticated visualization techniques it is possible to follow the interactions.... PubMed PMID: 27913448. 3. Cai, En, et al. "Visualizing dynamic microvillar search and stabilization ...

  18. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ...have introduced a GFP protein into your cells (visualized via the FITC channel) and you are trying to see...negative control. You can see how it is easier to visualize these four populations in the histogram plot in...gate off the histograms above, allowing you to visualize your data as a dot plot.  To summarize, if you...

  19. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...met with several individual and/or small groups, usually starting with the hiring manager. These interviews...setting, a conflict, and a resolution. Stories usually have a theme too, which is something not inherently... 5-6 stories for an interview, since one story usually has more than one theme and can be used to answer...

  20. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...has developed a new, plasmid-based biosensor to visualise changes in histone methylation in living cells...in the middle of a FRET pair, which is used to visualise the results. When methylation occurs at the histone-derived...sites. This new technology opens up a new way to visualize changes in gene expression and may lead to the...
Showing: 41 - 60 of 351 results