...academic scientists are starting to see the advantages of using social networking to start, build, and track...tell when some start-ups were about to be bought by seeing certain groups of people start to update their...my seven best tips beyond the basics to get you started. 1) Be visible and open to connections This is...add a personal note, you waste an opportunity to start things out right. Also, many people on LinkedIn ...include news, inclusion, culture and connections. Start your interview research there! 6) Your LinkedIn ...changes. If you are working, and all of a sudden you start updating your profile, everyone will think you are...

...Center. Susan Keown’s science communication journey started with something unexpected: termites. “I was really...says Susan. Her passion for insects lead her to start a PhD in entomology at the University of Maryland...know if she liked science writing. She decided to start a blog to test it out and discovered she not only...continued working for Palladian Partners remotely, but started looking for a new job because “it kinda sucked ...hired a full-time social media manager. “I usually start out my days looking through our Twitter feeds and...Susan’s number one piece of advice for getting started in science communication: “Find as many opportunities...post-grad school individuals, Susan recommends starting a blog, but with one caveat: “I hate telling people...

...for science policy folks is on Twitter- start an account and start engaging with the #scipol hashtag. Often... to college to graduate school, standing at the start of a science policy career was much more circuitous....  Who does science policy? I’ve intentionally started with the ‘who’ question because the science policy... from your alumni community is a great place to start. What is science policy? THE IMPOSSIBLE QUESTION...tenured professors. There’s never a bad time to start your science policy journey.  In fact, working in...time and that’s perfectly normal!  Action Items:  Start a career journal during your current job and note...this crowdsourced list of foundational texts as a starting point.  Assess and develop your own science policy...

...Pivot Back in March 2020, just four months after I started with Addgene, I was about to have a check-in with...resources we have, and we developed a great plan to start consistently knocking out high-quality videos for...the window. So, much like SynBio, it was time to start our Design-Build-Test-Learn cycles. If you’re not...space to shoot was another hurdle. As we slowly started to realize that we were going to be out of the ...wasn’t going to work for our other videos. For starters, I hadn’t anticipated nearly every webcam recording..., but still have control over focus, zoom, stop/start record, and allow me to see an image.  Remote video...camera, to control some basic functions, including starting and stopping recording).  Test: It was unbelievable...

... are a few out-of-the-box ideas that might jump start your learning. 1. Create a peer mentoring group ... career and life goals. For tips on how to get started see Form Your Own Peer Mentoring Group: A How-To...for the live audiences in your future.     3. Start something In almost every career talk I present,...charge before others do”. Candidates who have “started something” which they can share on their resumes... of things: led a grad or postdoc association, started a speaker series or biotech club, managed their...ukelele...perhaps you get the idea. What will you start? 4. Do informational interviews (on a schedule) ...you can’t wait until you imminently need a job. Start now. Informational interviews are 1:1 discussions...

... are a great starting point: Test different lengths of the primer binding site, starting with a length...General principles pegRNA design If you’re just starting out with prime editing, you’ll need to design ...different lengths of reverse transcriptase template, starting with about 10–16 nucleotides. For longer templates...editing (Anzalone et al., 2019). Figure 2: Starting lengths for optimizing your pegRNA primer binding...template during DNA repair. Test multiple nick sites, starting with sites about 50 bp upstream and downstream...editors bound to sgRNAs only drive nicking (right). Start with nicking sgRNAs ~50 bp from the prime editing...

...you need to start a whole new blot? Never fear: membrane stripping is here! Rather than start over, you...whole new gel and membrane transfer. Let’s get started! Before you begin In the best case, you know ahead...stripping and reprobing, maximize your signal by starting with the least-abundant protein and working toward...antibodies will require harsher stripping to remove, so start with the weaker binding ones first to preserve your... loss. If you are still optimizing your system, start with a mild stripping solution and move onto a stringent...incubation times, or try the stringent solution if you started with the mild one. Reprobe Once your membrane is...

...time PCR is a PCR technique used for measuring a starting DNA concentration using PCR. qPCR requires the... cycle.  To determine the concentration of the starting template DNA the fluorescent signal throughout...to a standard curve of amplified DNA of a known starting concentration. The cycle in which the unknown ...standard curve can be used to determine the amount of starting material in your sample.  qPCR can also be used...qPCR to quantify the concentrations of multiple starting DNA templates in one sample. It is important to...mutagenesis Molecular Diagnostics qPCR Quantify starting template DNA concentration Gene expression ...ultrasensitive and absolute nucleotide concentration of starting material AAV Titrations Allelic Variations Quantifying...

...all the basic and necessary elements in order to start using the MoClo system. The paper also gives quantitative...have any particular tips for those looking to get started with MoClo? Patron: The parts will be familiar ...the parts that you need from the kit and getting started making your own constructs with Golden Gate MoClo...conceptual barrier. Sometimes people don’t know how to start. They might want to use our promoters and terminators...they don’t have any prejudice about it. They just start and go ahead. I can see science speeding up. Addgene...engaging about science in that way for you? Patron: I started my Twitter account recently inspired by an academic...ate plants too. Think about how many people are starving. I think plant biology is massively underfunded...

...this idea, Drs. Koeris and Lu founded the biotech startup company, Novophage. As you'll hear Dr. Koeris explain... decided to develop phage for use in a biotech startup 12:16 - 15:15 : Why Sample 6 began to develop tests...Koeris to personally take on the risk of founding a startup company? 32:20 - 34:37 : Whose responsibility is...Sample 6 website Check out Dr. Koeris' Website, biotechstart.org Read Sample 6's Paper on Bacteriophage-based...Other Posts in Our Podcast Series Get Additional Startup Advice Learn How to Be A Good Manager from Addgene... startup...

...undergrad working with cancer cell lines. As I started planning out my own experiments in graduate school... As a busy researcher, you’ve probably already started coming up with time management techniques of your...make notes on as I progress. I’ve also recently started using Trello to manage larger, multi-person projects.... Knowing this, I hand write to-do lists at the start of each day to remind myself of the specific activities...where you’re pulled in a thousand directions at the start of the day, writing your to-do list the night before...listen to some of my favorite podcasts. JUST GET STARTED - One Harvard Business Review article on procrastination... have dinner plans at 6 pm, you had better get started early enough to finish everything you need to do...

...sales training and knew I had to start somewhere. I knew if I started with the Harvard Business Review...and cloning, I decided to pack it in. I had just started month 8 of my 3 year Post-Doctoral contract at ...interview. The simplest place I could think of to start was “Marketing for Dummies”. The fastest way to ...to learn something about marketing would be to start reading a broad simple overview, learn some jargon...academia, sales and marketing can be great places to start. Depending on what type of career you would like...

...easily started and easily credited. And nothing stops these one-time favors from becoming starting points... rewarding way of making science.   How we started Crowdfight-COVID19 Crowdfight was born as a collaborative... all over the world had signed up, and soon we started receiving dozens of requests every day.    ...suitability and availability; 3) Make the contact to start a direct collaboration between researcher and expert...are undergraduates or PhD students who are just starting their journey into science. Others are experienced...

... the internet, but start by downloading my handy list of suggestions.  Get started. Have regular meetings...elusive teachers, supporters and advisors? I tried to start a more formal mentoring program at my company, but...mentoring relationships.   If you would like to start a group mentoring program at your organization, ...provide a step-by-step, how-to guide to get one started. Advantages of the group mentoring format Adding... speaking to grad students and postdocs I have started to talk to trainees more about peer mentoring. ...

...because it has the advantage of starting out with a larger initial starting library size (up to 1012 members...To find an optimally binding sybody, you want to start out with a large and diverse pool of sybodies that...al., 2018). Find the Sybody Generation Toolbox! Starting with libraries and a biotinylated (for purification...et al., 2018. Ribosome display The Seeger lab starts with a “pre-enrichment” selection step using ribosome...Zimmermann et al., 2020). This step of the protocol starts out with anti-Myc antibodies bound to an ELISA ...

...often called “nontraditional,” helped prepare me to start CINSO. Here, I share some of the tips I learned ... had to take advantage of it. Blog and tweet I started my blog, Born to Science, as a graduate student...experience with my blog helped immensely when CINSO started brainstorming the resources we wanted to provide...my career path began to take shape, was when I started volunteering. I wasn’t sure yet what a career in...I volunteered at a place that made me happy. I started volunteering in the “Dinosphere” almost every week...the dinosaurs, and met wonderful people. When I started volunteering, I was worried about becoming one ...

...genome as a map with three coordinates: chromosome, start, and stop. For instance (chr3, 1, 10) indicates ...find all coffee shops near your house, excluding Starbucks. Taking a nerdy perspective, you might denote ...your search as follows: (my_house + coffee) - starbucks See how the notation works? The + operator denotes...above formula to a real-world experiment. We’ll start with ChIP-seq data from the ENCODE project, find... final step is to conduct our CRISPR screen. We start by infecting millions of embryonic stem cells (ESCs...CRISPRs Yours truly generated the DHS data set by starting with all of the DHS sites from 125 different human...

...creative problem-solving process?  You may want to start by carving out time to tackle it. It’s easy to get...Another strategy for your brainstorm session is to start with something completely unrelated to the issue...were instructed to use the three-ring binder as a starting point, the ideas generated were hardly novel. .... When told to use a roller skate as a starting point, their ideas scored 37% higher in originality. It...behind the study holds. To generate novel ideas, start with an out-of-the-box concept and pare it down ... the best it can be that it seems impossible to start at all. This is why perfectionists tend to also ...

.... I started watching the questions and preparing answers early in the day. Although I didn’t start answering...description of our AMA and Joanne’s preferred time to start answering questions. r/Science has pretty strict...was posted at 8:00 am and Joanne didn’t have to start answering until 5 pm). The ability to see the questions...Fortunately, Reddit Science is heavily moderated. I started watching the questions and comments go up early...things an early career scientist can do is to get started using LinkedIn early and brush up on best practices...

...was using.  I started to do little things to save reagents and money.  This was the start of my interest... a popular topic in the media.  At one point I started to become very aware of how little care I took ...time and money. My other stingy science project started when I realized I was pouring large agarose gels... a small portion of the gel.  What a waste?  I started looking for smaller and smaller gel apparatus until...