...Addgene depositor Indu Maiti has created a new and versatile binary Ti vector for both transient and stable...protein expression via Western blotting. Given its versatility and customizability, pSiM24 can be used not only...Dey, and Indu Bhushan Maiti. "pSiM24 is a novel versatile gene expression vector for transient assays as... Challenges in Plant Synthetic Biology Quick, Versatile Plant Transgenesis with GreenGate Plasmids Interview...

...flexibility, versatility, safety, and cost-effectiveness enable molecular biologists to broadly utilize plasmids...antibiotic resistance gene. In contrast, plasmids utilized in the lab are usually artificial and designed...gene of interest into a vector, scientists may utilize one of a variety of cloning methods (restriction...

...valuable laboratory tool are still an area of active research. New details are still being uncovered, and different...This helps explain why Cas9 can’t locate PAM sites until after RNA loading: the structure to interrogate ...produce SuperFi-Cas9, a high-fidelity variant that still retains high cleavage efficiency (Bravo et al., ...et al., 2014). New discoveries on this topic are still being made! Conclusion Finally, we can summarize...

...Science is failing over and over and over again until you don’t.” As every researcher can attest to, science... that the superheroes win in the end. But we’re still invested in seeing how they do it.    Finding...within that process of failing, over and over again until you don't, of trying to understand your completely... said that even when we know the ending, we can still get invested in the journey? The same thing applies...

...quantify Cas9 cleavage, thus exhibiting its versatile utility in quantifying multiple gene editing activities...2018. PubMed: PMID 30220597. ACE system: A versatile in vivo fluorescent reporter for rapid quantification...fluorescence-based quantification of base editors that utilize dCas9 fused to cytidine deaminases like APOBEC....

...luciferase was published in 1985, several studies utilized luciferase as a genetic reporter in plant and ...None Yes Luminous Japanese ostracod(Cypridina noctiluca)Sea firefly(Cypridina hilgendorfii) 465 62 Cypridina... produce light, often with a luminometer or scintillation counter, and the activity measurement can then...interest permits translation of functional luciferase, until miRNA target and degrade the mRNA sequence, resulting...

... not naturally exist in mammals, scientists can still reap the benefits of plasmid-based research using... a reporter and we will be covering this ever-versatile fluorophore in a later post, so stay tuned! Transient...that you may not want the plasmid depleted, but still want to use transient transfection methods. Since...selection agent should not be added to culture media until 24-48 h post transfection when creating stable cell...

...focus of the application was changed, but Sample6 still leverages the core engineering and scientific concepts...into the local academic community. However, Mike still could not remove himself from the idea of starting... importantly, “the company does not become real until someone takes the risk full-time and abandons all... many universities throw out old equipment that still works. “We use functional spectrometers from the...

.../Cas9 effector (LACE) system, the Gersbach lab utilized a similar strategy to develop an optimized photoactivatable...repair (HDR). This system is unique in that it utilizes a “split” nuclease - the authors fragmented Cas9...the figure below. Their most successful design utilized Magnet photoswitchable proteins derived from the.... aureus Cas9 (saCas9) proteins, showing the versatility of this approach. This study generated the first...formed 20-40 min later. The CASANOVA system is versatile in that it can be adapted to regulate gene editing...

...experiments in S phase zygotes at 18 hr post fertilization. They observed higher targeting efficiency than...41/42 embryos were non-mosaic at 3 days post fertilization. Since Ma et al. designed a repair template ...recessive gene. Ma et al. claim that CRISPR would still be helpful in increasing viable embryos, especially... in terms of editing success rate. Would CRISPR still be worthwhile for a given disease if it increased...

...chemically modified to resist degradation while still effectively serving their function of guiding Cas9...The photoactivatable counterpart to this system utilizes a gRNA which is photocaged and unable to engage...initiate targeted editing events. Both technologies utilize chemical modifications to the RNA to control the...molecule) you will not have that option, but you can still choose what dye to use  What to do once you’ve chosen...

... that the majority of attendees were scientists still working at the bench. It was a fantastic opportunity...unintended themes of the meeting was how much we still don’t know about RNA. One talk that really brought...about his work on the 500+ human tRNA genes and, until now, we've overlooked regulatory fragments of these... summed up this conference—nucleic acid science still has many surprises for the researchers in this field...

... cards, incorporation, laboratory lease, etc. I still flip to that page in my lab notebook from time to...worked weekends and evenings on the project, but still continued to generate new ideas as they popped into... as food but support GMOs that result in a new utility, was there a business opportunity out there for...figure out how we would accomplish the color change until a few months later. RevBio accelerates with help...

...limited to restriction enzymes - although many labs still love using them! With so many technologies currently... might be for you. This methodology relies on versatile Type IIS restriction enzymes. These cut outside...mix. Nonetheless, Gibson assembly is a highly versatile and efficient way to generate a wide variety of... is extremely efficient due to the use of the versatile type II restriction enzymes. In Golden Gate cloning...

...comes to fruit flies Drosophila start life as fertilized eggs that develop into embryos and then become...about one week from the initial deposition of the fertilized egg by its mother. Inside the pupal case, the...important to isolate females from males while they are still virgins. Females begin mating ~6 hours after hatching... convince him to use Twitter @jonchowphd but he still reads “#” as a “pound symbol”. Additional Resources...

...there are benefits to optimized libraries that utilize only a few sgRNAs per gene. These libraries provide...., 2016, Sanson et al., 2018). These libraries utilize optimized sgRNAs that were designed based on the...while bypassing these issues. CRISPRi libraries utilize an engineered Cas9, which contains multiple point...transcription factors. The Root and Doench labs utilized tracrRNAs with two PP7 stem loops to recruit additional...

...developed in Saccharomyces cerevisiae in 1989 and are still used extensively to screen for molecular interactions...instance, the DBD of the fusion protein shown is still able to bind to the upstream activation sequence...has occurred. Although the original Y2H systems utilized the yeast Gal4 activator, bacterial LexA DBD and...bait and prey protein fusions to demonstrate the utility of the Y2H system, it is more common to use many...

...jellyfish for millions of years; however, it took until the 1960s for scientists to begin to study GFP and...more about GFP, how scientists have evolved this versatile protein to suit their experimental needs, and ...A. Victoria and expressed in any organism while still maintaining fluorescence. The protein structure,...mainstay in molecular biology. Scientists can easily utilize GFP-containing plasmids as a means to many functional...

...husbandry and ability to produce abundant, externally fertilized, transparent progeny (Figure 2). These features...regulate processes from development to metabolism to fertility. Several zebrafish-specific gene expression databases...screens produced ~1,500 zebrafish mutants that are still used in labs across the globe (Nüsslein-Volhard ...tracing Over a period of 24 hours, the externally fertilized zebrafish zygote transforms into an organism ...

... and put it in a fertilized egg to get edited embryos (replacing the nucleus is still very difficult)....diverse organisms. Methods for genetic manipulation still need to be developed for each organism, but researchers...targeted gene mutations in anoles lizards or in any reptiles at all. But new success is coming with CRISPR/...