Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 214 results for: TIR

Showing: 41 - 60 of 214 results

  1. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...of interest, or a defined cDNA library (e.g. the entire proteome of a particular cell type, or a subset...at the same time in order to try and access the entire interactome of a set of proteins at once.  However...

  2. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...in ultra-long read mode), enough to sequence an entire plasmid in one read. Runs can be completed in anywhere...sequencing, as well as the ability to sequence an entire viral genome in one read. We also use Nanopore ...

  3. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...over half of which, when implemented, impacted the entire company. It is important to note that the person...product team is an incredibly innovative group. Our entire company benefits from their policy! Perfectionism...

  4. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...of the edited sample. You can also download the entire analysis as a ZIP file by clicking the Download...the inferred distribution of indel sizes in the entire edited population of genomes. Hovering over each...

  5. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ... and therefore may need fewer resources for the entire translation process. For example, during high growth...inappropriate locations or fail to make it through the entire transcript resulting in the production of nonfunctional...

  6. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...proteins may not transfer precisely evenly across the entire blot. So how can you accurately compare different...your approach) as your final blot. Quantifying the entire lane of protein provides a loading control that...

  7. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...Yeast are also the first eukaryotic genome to be entirely sequenced and is very amenable to genetic manipulation...utilized the worm to study calcium signaling in the entire adult brain, using a plasmid that expresses a genetically...

  9. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ... or guide RNA blocking mutations? This depends entirely on the locus you are editing, and if the blocking...experiments with blocking mutations, we still weren’t entirely happy with our editing outcomes and in many cases...

  11. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...and it causes a large deletion that removes the entire exon that codes for the epitope recognized by your...you very similar results, while the third looks entirely different (B), giving you confidence that your...

  12. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...and the rise of killer machines that exterminate entire humanity were the two most popular topics that ...negative depictions serve as cautionary tales for the entire scientific community and  remind us to always consider...

  13. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...variable region of an antibody does not recognize the entirety of the target protein: instead, it recognizes ...between the IgG antibody and scFv. Because the entire antibody molecule is not necessary for antigen ...

  14. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...hypothesized that the CGBE edit process occurs entirely while Cas9 is bound, keeping the two DNA strands...regions that were already accessible. It's not entirely clear why this is, but it might point to a more...

  15. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...backbone, sidestepping the problem of HDR vs. NHEJ entirely. The first two classes of base editors were cytosine...base editing, has allowed scientists to replace entire sequences of varying lengths. Base editing publication...

  16. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...and injected at several hundred sites across the entire mouse brain. Traced neurons expressed a fluorescent...close or distal to the injection site across the entire brain, allowing for an unprecedented number of ...

  18. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    Published
    Dec. 2, 2016, 3:30 p.m.
    ...win a hot chocolate party that will warm up the entire lab. We’ll choose 4 winning submissions and deliver...

  20. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ...present our own data on plasmid deposits and distirbution for these fast moving fields.  Addgene is a ...
Showing: 41 - 60 of 214 results