We narrowed to 61 results for: UNK
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TypeBlog PostPublishedFeb. 4, 2020, 2:15 p.m....https://doi.org/10.1128/aem.00990-19 Liljeruhm, J., Funk, S. K., Tietscher, S., Edlund, A. D., Jamal, S.,...
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Plasmids 101: Optimizing Plasmid Yields
TypeBlog PostPublishedMay 26, 2016, 2:30 p.m....low yields from a plasmid whose copy number is unknown, it is entirely possible that you are working with... -
Droplet Digital PCR for AAV Quantitation
TypeBlog PostPublishedJan. 16, 2020, 2:18 p.m....dilutions onto our ddPCR plate. As the titer is unknown, each dilution should fall within the range of ... -
Tips for arabidopsis transformation
TypeBlog PostPublishedOct. 25, 2018, 1:23 p.m.... culture into a container that is wide enough to dunk your plant’s flowers inside. 50 mL falcon tubes ... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog PostPublishedAug. 9, 2016, 2:30 p.m....dark matter in the genome. There are countless unknown regions yet to explore. I hope that this brief ... -
Plasmids 101: Protein tags
TypeBlog PostPublishedDec. 11, 2014, 4:26 p.m....proteosomes to degrade what the cell might consider junk protein. Though there are a number of chemical and... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog PostPublishedJuly 2, 2019, 12:51 p.m....sequenced. Guide RNAs targeting several known and some unknown regulators of neuronal differentiation were enriched... -
How to Write a Scientific Review Article
TypeBlog PostPublishedFeb. 16, 2017, 3:30 p.m.... writing: initially I felt that I needed a huge chunk of time to sit down and get anything done, so unless... -
CRISPR Activation: A Practical Guide
TypeBlog PostPublishedAug. 18, 2016, 2:30 p.m....isoform(s) common to a particular cell type are unknown or not readily available. By activating from the... -
Tips for CRISPR Gene Editing in Mice
TypeBlog PostPublishedJune 28, 2016, 10:59 a.m....why some gRNA’s work better than others remain unknown, but fret not! The key to saving time and energy... -
Transferable Skills Guide: Time Management
TypeBlog PostPublishedDec. 5, 2017, 1:52 p.m....broken down time management into three conceptual chunks: prioritization, planning, and procrastination.... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog PostPublishedSept. 14, 2016, 2:30 p.m.... pull-down procedure for the identification of unknown GEFs for small GTPases." Small GTPases 7.2 (2016... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog PostPublishedJune 23, 2016, 2:30 p.m....phosphatase treatment. If a candidate phosphatase is unknown, CIP or other generic phosphatases may dephosphorylate... -
Viral Vectors 101: Optogenetic Tools
TypeBlog PostPublishedJune 27, 2023, 1:15 p.m....particular receptor or when a signaling pathway is unknown. Figure 3: Chimeric OptoXR incorporating... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog PostPublishedNov. 2, 2021, 1:15 p.m....achieved. The fact is that there are many known and unknown factors that affect vector performance in each ... -
Transferable Skills Guide: Teamwork
TypeBlog PostPublishedNov. 28, 2017, 2 p.m.... of my life that had me listening to Simon & Garfunkel’s “I Am A Rock” on repeat in my bedroom. I realized... -
Finding Your Science Policy Path
TypeBlog PostPublishedSept. 22, 2020, 1:15 p.m....science policy career was much more circuitous and unknown. In fact, in less than two years of walking away... -
A History of Genome Engineering in Popular Culture
TypeBlog PostPublishedFeb. 25, 2020, 2:15 p.m....Colin MacLeod, and Maclyn McCarty’s experiment debunked the myth that protein is the carrier of genetic... -
15 Hot Plasmids from 2017
TypeBlog PostPublishedJan. 9, 2018, 3:02 p.m....from a polycistronic cassette. However, it was unknown whether variations in gene expression from these... -
22 Hot Plasmid Technologies from 2014
TypeBlog PostPublishedJan. 6, 2015, 5:21 p.m....neurons. The paper describes five previously unknown channelrhodopsins from different species: Stigleoclonium...