We narrowed to 53 results for: alp

1. New Tools Enable CRISPRa for Neuroscience Applications

   Type

   Blog Post

   Published

   July 2, 2019, 12:51 p.m.

   ...promoters, including calmodulin-dependent kinase II alpha (aCaMKII; excitatory neurons) and Synapsin-1 (pan-neuronal...

2. Plasmids 101: Stringent Regulation of Replication

   Type

   Blog Post

   Published

   June 20, 2024, 1:15 p.m.

   ... Mizobuchi, K. (1998). Copy number control of IncIalpha plasmid ColIb-P9 by competition between pseudoknot...

3. Data Freedom: The Expansion of Data Sharing in Research Publications

   Type

   Blog Post

   Published

   Aug. 5, 2014, 6:51 p.m.

   ...diseases. His spare time is spent trying to raise the alpha isoform of GSK-3 to the same (deserved) stature ...

4. Plasmids 101: The Promoter Region – Let's Go!

   Type

   Blog Post

   Published

   April 3, 2014, 8:05 p.m.

   ...mammalian expression from human elongation factor 1 alpha Constitutive  Tends to give consistent expression...

5. Fluorescent Proteins 101: When GFP lets you down

   Type

   Blog Post

   Published

   Aug. 23, 2018, 12:05 p.m.

   ...successfully generated several functional heterotrimeric G-alpha subunit fusions that do not tolerate N- and C-terminal...

6. Hot Plasmids: Summer 2024

   Type

   Blog Post

   Published

   Aug. 27, 2024, 1:15 p.m.

   ...peptides (3HB: 11-nm 3-helix bundle; SAH: 60-nm single alpha helix) and target capture module (SpyTag-GFP nanobody...

7. CRISPR 101: Epigenetics and Editing the Epigenome

   Type

   Blog Post

   Published

   June 24, 2020, 5:45 p.m.

   ...Transcriptional repression DNA Methyltransferase 3 Alpha (DNMT3A) Vlatka Zoldoš’ lab has deposited pdCas9...

8. Plasmids 101: Repressible Promoters

   Type

   Blog Post

   Published

   Dec. 20, 2022, 2:15 p.m.

   ...expression system for sphingomonads and other Alphaproteobacteria. Appl Environ Microbiol. 2013 Nov;79(21):...

9. Plasmids 101: Restriction Cloning

   Type

   Blog Post

   Published

   Feb. 18, 2016, 3:42 p.m.

   ...ligation reaction into competent cells such as DH5alpha or TOP10. If using much less total DNA (<1ng) ...

10. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type

    Blog Post

    Published

    Oct. 8, 2020, 1:15 p.m.

    ...human speech near the laminar airflow, the human scalp, sneezing, coughing, etc. Also, a constant in-flow...

11. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type

    Blog Post

    Published

    Dec. 15, 2016, 3:57 p.m.

    ...Recombineering, EcoSal Plus 2016. doi:10.1128/ecosalplus. PubMed PMID: 27223821. 4. Sharan SK, Thomason...

12. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type

    Blog Post

    Published

    Nov. 6, 2018, 1:12 p.m.

    ...Thorel, F., et al., Conversion of adult pancreatic alpha-cells to beta-cells after extreme beta-cell loss...

13. 28 Hot Plasmid Technologies from 2015

    Type

    Blog Post

    Published

    Dec. 23, 2015, 3:30 p.m.

    ...Rtn4a-GFP (tubular ER) Microtubules mCherry-alpha-tubulin Early Endosome Vacuolar Compartment...