We narrowed to 45 results for: c myc del 2
-
TypeBlog PostPublishedNov. 19, 2013, 2:37 p.m....partial gene deletion, overexpression (by promoter substitution), and tagging at either the N- or C-terminus...Pringle’s former lab at UNC Chapel Hill. Bähler et al.(2) describe a modular collection of plasmids for a wide...protein-protein interactions via FRET. Sidae Lee, Wendell Lim, and Kurt Thorn at UCSF have recently developed...C-terminus (3xHA, 13xMyc, GST, or GFP). Longtine et al.(3) describe a complimentary set of plasmids for use in...
-
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog PostPublishedMay 2, 2019, 12:41 p.m....presented in a series of quick (10 minute) or very quick (2 minute) talks so we got to hear from everyone. Addgene...evolutionary tree at least 6-7 times and on at least 2 separate continents. Mimulus (Andrea Sweigart) Monkeyflowers...habitats - even 50 meters from Old Faithful in 60 °C soils. Dendrobatidae (Lauren O’Connell) Poison frogs...Organisms, Part 2 ...our other model organism blog posts: Five Popular Model Organisms, Part 1 Five Popular Model Organisms...the spiny purple sea urchin. It’s not a standard model organism, but it was easy to harvest eggs and sperm...So, think beyond Drosophila melanogaster or Saccharomyces cerevisiae (both fine research organisms) - ... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog PostPublishedMay 14, 2024, 1:15 p.m.... Multiple Introns.” Plant Communications, vol. 2, no. 2, Elsevier BV, Mar. 2021, p. 100135. Crossref, ...Constructs.” PLoS ONE, edited by Jean Peccoud, vol. 6, no. 2, Public Library of Science (PLoS), 18 Feb. 2011, p... vol. 5, no. 10, American Chemical Society (ACS), 2 May 2016, pp. 1059–69. Crossref, https://doi.org/10.1021...doi.org/10.1371/journal.pone.0197185. Chamness, James C., et al. “An Extensible Vector Toolkit and Parts Library...of Plant Genomes.” The Plant Genome, vol. 16, no. 2, Wiley, 9 Mar. 2023. Crossref, https://doi.org/10.1002...Crossref, https://doi.org/10.1186/s12870-020-02388-2. Stuttmann, Johannes, et al. “Highly Efficient Multiplex...Crossref, https://doi.org/10.1038/s41598-019-46171-2. Resources on Addgene.org Modular Cloning (MoClo... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog PostPublishedAug. 15, 2017, 1:24 p.m.... fluorescent proteins expressed in the specimen. 2. Fluorescence Activated Cell Sorter (FACs) A machine...photostability of your fluorescent protein when using 2-photon microscopy as this hasn't been well reported...system and its dynamics. References 1. Peters, Nathan C., et al. "In vivo imaging reveals an essential role...PMID: 18703742. PubMed Central PMCID: PMC2606057. 2. Chtanova, Tatyana, et al. "Dynamics of neutrophil...help immunologists create even better tools and models to further our understanding of the immune system...during the development and disintegration of mycobacterial granulomas." Immunity 28.2 (2008): 271-284. ...presentation and T cell effector function in mycobacterial granulomas." Immunity 34.5 (2011): 807-819. ... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog PostPublishedSept. 14, 2016, 2:30 p.m.... easy for a researcher to quickly generate N- and C-terminal epitope tag fusions to a protein of interest...wheat germ, HeLa, and Leishmania (Gagoski, et al 2015[2]). The proteins encoded in this set range in size ...to generate Illumina sequencing libraries using a 2-step PCR-based protocol and are compatible with single-end...quickly study your gene of interest in a variety of model systems, thereby opening your research to a variety...initiation heavily dependent upon the core of a Shine-Delgarno sequence found at the 3’ end of the leader sequence...predictable based solely on the promoters and Shine-Delgarno sequences used in this so-called bicistronic design...et al. "Adaptation of Enterococcus faecalis to daptomycin reveals an ordered progression to resistance....