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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 137 results for: ckb

Showing: 41 - 60 of 137 results

  1. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...sequence and protein data, as well as available backbone sequences. All of these resources allow QC scientists...feature highlighted by the deposited scientist  The backbone elements based on the information provided and...

  2. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...the desired mutation and excision of the vector backbone. To do this, rhamnose is added which induces expression...point will be those that have excised the vector backbone. Vectors pTOX4, pTOX5, and pTOX6 express the ...

  3. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...tagging the kinesins KIF5A and KIF5B as well as backbones for generating other SNAPf-, CLIPf-, and CLAP-...biosensor in optimal proportions from a vector backbone that allows lentiviral packaging as a single payload...

  4. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...plasmid backbone that was designed specifically for transient expression (find some useful backbones here...

  5. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...protocol!  First, the authors created the pMAK backbone by modifying pMAL-T-Avi-His/BirA, a vector commonly...consisting of CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots and ...

  6. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...not leave much flexibility for discrepancies in backbone sequences. Most theoretical sequences submitted...deposits that contain many plasmids within the same backbone. The flexible auto-alignment, plus additional ...

  7. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...compare their options for tools such as empty backbones, lentiviral packaging systems, and luciferase ...Plasmids Tetracyline Inducible Plasmids Empty Backbones Bacterial Expression Systems AAV Plasmids ...

  8. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...track: Compatibility: Double-check your plasmid backbone and expression strain to make sure they are compatible...below or browse Addgene’s collection of empty backbones.   Our Most Popular Plasmids 101 Posts: Origin...

  9. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...respective species. Addgene carries >30 “empty gRNA” backbones which contain a plant pol III promoter and gRNA...plants and many of the aforementioned empty gRNA backbones also contain Cas9, which enables expression of...

  10. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...interest (Figure 2). 1. The phosphoserine OTS The backbone of the phosphoserine OTS, Sep-OTS, contains the...using the PhosTag system (see below). This plasmid backbone can also be cloned with an ORF of interest for...

  11. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...chemically modify target bases without breaking the DNA backbone, making them efficient and precise tools for altering...which incorrect nucleobases are removed from the backbone, creating abasic sites for further repair.   ...

  12. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...thymine in this case) is only bound by the phosphate backbone. These PAM bases must be present for Cas9 to unwind... both lobes bind to the DNA and RNA phosphate backbones, allowing Cas9 to verify the structure of the ...

  16. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ... genes respectively, use lentiCRISPR v2 as the backbone for their many gRNAs. The high titer lentivirus...

  17. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ...service. This service features 210 plasmids: empty backbones, fluorescent protein tags, SARS-CoV-2 plasmids...

  18. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...reaction. STAgR is highly customizable as vector backbones, and gRNA structure, numbers, and promoters can...

  20. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...for microscopy enthusiasts! Addgene has empty backbones containing GFP variants used by Sattarzadeh et...
Showing: 41 - 60 of 137 results