We narrowed to 246 results for: fas

...Cysteine-less, fast split intein; N-terminal CLm and C-terminal Aes gp41-1 Split inteins A small, fast split ...High rate constant— time is money and things move fast in biology. Slow reactions are only good enough ...

...ordering, making it a toss-up as to whether it’s faster to order or try to make it in your lab (but if ... you need a lot of plasmids, it’s almost always faster to order from us!) Ask the PI If you want a plasmid...working at speed or money if budget is a concern. The faster methods, like Gibson assembly, are typically more...

...amino acid peptide, the CRY2clust domain enables fast and reversible oligomerization when fused to a protein...termination of light stimulation. CRY2clust enables faster assembly and disassembly dynamics, higher sensitivity...optogenetic system (OptoSTIM1), caused two-fold faster changes in intracellular Ca2+ levels than the original...

...Kinetics How fast is the process you would like to monitor? Neurons, for example, have unusually fast calcium...three GCaMP types based on their kinetics: GCaMPf (fast) GCaMPm (medium), GCaMPs (sensitive but slower)....

...have very different disease phenotypes, are two fascinating examples of epigenetics in the form of imprinting...lead to excessive eating and obesity. What is fascinating is that both syndromes can present without any...treat complex or multifactorial diseases, enabling faster diagnosis and treatment and improving patients ...

...determined by their homology. You might also consider FastCloning, first described by Chaokun Li and colleagues...the prices of the additional enzymes required. FastCloning may be the least expensive of all the options...Addgene!). References 1. Li, Chaokun, et al. "FastCloning: a highly simplified, purification-free, sequence-and...

...these viruses. The cells are also easy to cultivate, fast-growing, and widely available. The viruses Lentiviruses...like HEK-293T cells, they are easy to cultivate, fast-growing, and widely available. Unlike mammalian ...adenoviruses and the origin of HEK 293 cells. The FASEB Journal, 16(8), 869–871. https://doi.org/10.1096...

...viral DNA for quality control*. This gives us a faster turnaround time than short-read sequencing, as ...The workflow converts the raw sequencing data to FASTQ format, cleans and normalizes reads using the BBTools...program called Geneious. Within Geneious, the raw FASTQ reads are used for either guided or new de novo ...

... 100% negative prediction rate. It is also the fastest test that has been developed so far.  A similar...challenging samples as in Two-Step-SHERLOCK or in a fast, high-throughput manner in one step as One-Pot-SHERLOCK.../2020.04.07.028167 Broughton JP, Deng X, Yu G, Fasching CL, Servellita V, Singh J, Miao X, Streithorst...

...stimulate at a fast frequency without missing an action potential, then a channel with fast kinetics may...V. S., Deisseroth, K., & Hegemann, P. (2010). Ultrafast optogenetic control. Nature Neuroscience, 13(3...

...accolades that would launch our careers in science. Fast forward nearly 20 years and I am known by some colleagues...in Jefferson’s case, even a lot about everything. Fast forward a few hundred years and there are currently... will not only get your paper out the door much faster, but getting helpful feedback from other people...

... require high sequencing read counts making them fast and viable options for multiplex sequencing in many... for all DSBs, doesn't require high read counts, fast multiplexing Requires delivery of dsODN (potentially... (e.g. dsODN), doesn't require high read counts (fast multiplexing) Requires large cell population, senstive...

... of CRISPR/Cas9 technology are coming along at a fast and furious pace. In December, as we've blogged ...it before, but CRISPRs allow research to move so fast." On the very same day the monkey story hit the ...

...researchers worldwide. “COVID-19 has spread incredibly fast and without any notion of national boundaries. To...policies and ultimately treatments in a timely fashion.”  With this in mind, Bourque and Tara Moriarty...

... (e.g., 100 nm) of DA. In addition, they exhibit fast kinetics (10 ms on and 100 ms off), enabling detection...PMCID: PMC426888. Patriarchi, Tommaso, et al. "Ultrafast neuronal imaging of dopamine dynamics with designed...

...imaging of voltages. This includes monitoring of fast bursts, as well as slow up/down voltage states in... Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy. Cell...

...budgets, and we made that happen. Others needed a faster way to find plasmids with specific sequences, so...the background, but you'll feel their impact in faster turnaround times and more reliable service. We ...

...a simple egg is packed with information. I am fascinated with how developmental programs unfold, absorbing...comparative anatomy showed me that some of the most fascinating biology is found in weird and rare organisms ...

... the ability to do DNA synthesis, is evolving so fast that it is a really nice opportunity to study it...happens when technological change happens really fast,” Thompson says. To do this, Thompson reached out...

...more about one another. They also provided both breakfast, lunch, and snacks. At the end of the workshop...experiences like workshops at Lab Central. Because of the fast pace of work in the Addgene lab, we don’t often ...