...Kinetics How fast is the process you would like to monitor? Neurons, for example, have unusually fast calcium...three GCaMP types based on their kinetics: GCaMPf (fast) GCaMPm (medium), GCaMPs (sensitive but slower)....

...determined by their homology. You might also consider FastCloning, first described by Chaokun Li and colleagues...the prices of the additional enzymes required. FastCloning may be the least expensive of all the options...Addgene!). References 1. Li, Chaokun, et al. "FastCloning: a highly simplified, purification-free, sequence-and...

... 100% negative prediction rate. It is also the fastest test that has been developed so far.  A similar...challenging samples as in Two-Step-SHERLOCK or in a fast, high-throughput manner in one step as One-Pot-SHERLOCK.../2020.04.07.028167 Broughton JP, Deng X, Yu G, Fasching CL, Servellita V, Singh J, Miao X, Streithorst...

...stimulate at a fast frequency without missing an action potential, then a channel with fast kinetics may...V. S., Deisseroth, K., & Hegemann, P. (2010). Ultrafast optogenetic control. Nature Neuroscience, 13(3...

... require high sequencing read counts making them fast and viable options for multiplex sequencing in many... for all DSBs, doesn't require high read counts, fast multiplexing Requires delivery of dsODN (potentially... (e.g. dsODN), doesn't require high read counts (fast multiplexing) Requires large cell population, senstive...

...accolades that would launch our careers in science. Fast forward nearly 20 years and I am known by some colleagues...in Jefferson’s case, even a lot about everything. Fast forward a few hundred years and there are currently... will not only get your paper out the door much faster, but getting helpful feedback from other people...

... of CRISPR/Cas9 technology are coming along at a fast and furious pace. In December, as we've blogged ...it before, but CRISPRs allow research to move so fast." On the very same day the monkey story hit the ...

...researchers worldwide. “COVID-19 has spread incredibly fast and without any notion of national boundaries. To...policies and ultimately treatments in a timely fashion.”  With this in mind, Bourque and Tara Moriarty...

...imaging of voltages. This includes monitoring of fast bursts, as well as slow up/down voltage states in... Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy. Cell...

... (e.g., 100 nm) of DA. In addition, they exhibit fast kinetics (10 ms on and 100 ms off), enabling detection...PMCID: PMC426888. Patriarchi, Tommaso, et al. "Ultrafast neuronal imaging of dopamine dynamics with designed...

...a simple egg is packed with information. I am fascinated with how developmental programs unfold, absorbing...comparative anatomy showed me that some of the most fascinating biology is found in weird and rare organisms ...

... the ability to do DNA synthesis, is evolving so fast that it is a really nice opportunity to study it...happens when technological change happens really fast,” Thompson says. To do this, Thompson reached out...

...more about one another. They also provided both breakfast, lunch, and snacks. At the end of the workshop...experiences like workshops at Lab Central. Because of the fast pace of work in the Addgene lab, we don’t often ...

...easy to manipulate, it’s affordable, and it grows fast to boot. The yeast strains S. cerevisiae and S. ...to culture and easy to work with Grows extremely fast (doubling time of ~ 2 hours) Amenable to genetic...

...Scientists need fast access to the tools required to study SARS-CoV-2, but with many academic research... the scientific community. Addgene was awarded a Fast Grant to ensure that the scientific community will...

...throughput and faster. It’s also easy to run things in parallel. Addgene: Besides being fast and efficient...

...Nucleic Acids Research. 2018 PubMed PMID: 29924347 Faster proximity labeling with TurboID and miniTurbo Article...frequently used for PL were APEX2 and BioID. APEX2 is fast, tagging proximal proteins within minutes, but requires...

...industrial labs accelerate research and to help find a faster solution to the COVID-19 pandemic. Though we’ve...instrumental in achieving our mission to help science move faster in the middle of a global pandemic. And the reach...

...schedule might work. If not, perhaps a 3 hour breakfast once a month. All participants must commit to ...each other practice meeting people – compete for fastest to set up a coffee date or most cards collected...

...-2 virus quickly and openly. By sharing results faster and more broadly, scientists can receive recognition...Preprints are all about sharing scientific knowledge faster and more openly - which is also our mission here...